PH level of Amylase Background info: What is Amylase? Amylase is an enzyme that helps digest carbohydrates. It is produced in the pancreas and the salivary glands. (Dugdale & Longstreth‚ 2011) Factors Affecting Amylase: Things that affect the efficiency of Amylase are temperature and pH levels. (Wikimedia Foundation‚ Inc‚ 2013) Function in the body: The function of Amylase in the human body is to break down plant-based starch sources. Therefore‚ providing the human body with more
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Identification of unknown a-Amylase through testing different temperatures and pH values to detect the absorbance of maltose. Introduction: Enzymes are biological catalysts‚ mainly proteins for this experiment‚ generated by an organism to speed up chemical reactions. They have active sites on which the substrate is attached‚ and then broken up or joined. For this experiment we are going to work with the enzyme a-amylase. Amylase is an enzyme that breaks starch down into sugar. Amylase is present in human
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between temperature and the enzyme activity of amylase. This was achieved by attaining amylase enzyme‚ starch solution and potassium iodide (determines if enzymes hydrolyses the starch solution)‚ water bath and a hot plate. The temperatures used for this experiment were room temperature‚ 37oC‚ 60oC‚ 80oC‚ and 90oC. The hypothesis developed was that as the temperature increased‚ so will enzyme activity. Therefore‚ the ability of the enzyme to break down the starch solution will occur at a faster
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change in pH levels has on a particular enzyme‚ in this case amylase. Hypothesis: In this investigation I expect as the pH reaches the optimum level‚ the rate of reaction will be fastest‚ compared to other pH levels. It is also suspected that after the enzyme has reached optimum level the enzyme activity will decrease. Through further study of the optimum level of amylase I found that the enzyme usually has an optima pH of 8. It is known that the pH of an enzymes environment will affect its activity
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Suzuki‚ Laurent Santos‚ Val Justin D.M. Tongco* College of Science‚ University of Santo Tomas‚ Manila Philippines Abstract Salivary amylase‚ found in humans‚ is enzyme that catalyzes the hydrolysis of starch into simpler compounds. Its enzymatic activity is affected by several factors‚ such as temperature and pH. The rates of enzymatic activity of salivary amylase in different temperatures and pH were measured and resulted to be very near 50 C and 7 respectively. However‚ due to some errors that
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Biosynthesis of Starch Introduction: In this lab we learned the concept and procedure of synthesizing starch. We also learned the effects of pH and temperature on the reaction rates of amylase.. In the process of the synthesis lab we learned phosphorylation using a potato‚ which was what we synthesized. The phosphorylation took place after the addition of primer. There are two different types of starches used are amylose and amylopectin. To test for the presence of starch‚ the Starch Test is used
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1 The Limits of Amylase 2-1-14 Abstract This report explains the purpose of this experiment in a way that conveys information to the reader about Amylase’s ability to withstand acidic or basic pH. To do this‚ two test tubes were both filled with 5mL of a 5% amylase solution. The first one was filled with an acid‚ while the other was filled with a base. After dropping liquid Iodine and Benedict’s solution into each one‚ the tube with a basic pH tested positive for glucose. The acidic solution
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Fungal Amylase and hydrolysis of Starch Abstract This experiment consisted of setting up a control group of starch in various temperature and then placing both fungal amylases and bacterial amylases in a mixture of starch and placing the solution of amylase and starch in various temperatures of water. After a certain amount of time- different amount of time needs to be used in order to have reliable results- iodine is added in a well on spot plates‚ then two drops of the mixture of amylase-starch
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of Human saliva (Salivary amylase) against Temperature Proponent: Ian Angelo P. Dela Cruz BS-Biology 1-3 Prof. McJervis S. Villaruel Professor – BIOL2015(Lab) ABSTRACT This report entitled “Enzymatic activity of Human saliva (Salivary amylase) against temperature” aims to know and observe the enzyme activity of the human saliva. The research only included the use of starch-agar as the medium to observe enzyme activity during the experiment. Five starch-agar plates were prepared
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how starch and cellulose are treated to allow them to be used by the yeast? One potential ethanol feedstock is starch. Starch molecules are made up of long chains of glucose molecules. Thus‚ starchy materials can also be fermented after breaking starch molecules into simple glucose molecules. Examples of starchy materials commonly used around the world for ethanol production include cereal grains‚ potato‚ sweet potato‚ and cassava. A great amount of ethanol fuel is currently produced by starch
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