Introduction Spectrophotometer is an optical instrument that measures and shows how much light energy is transmitted by a substance in solution at different wavelengths of radiant energy. Light passes into a monochromator where only a very narrow range of wavelengths can pass through. From there‚ light is transmitted through a sample solution‚ and on to a phototube where the light energy is converted to an electric current that is registered on a meter. To measure the amount of light absorbed
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10 mmol/L | Albumin | 30 g/L | Glucose | 12 mmol/L | Amylase | 5000 U/L --- Normal Range: 60-180 U/L | Serum: Comment: The diagnosis of acute pancreatitis is based on the clinical history‚ evidence of inflammation is known usually by computerized tomography (CT scan) and the finding of a high serum (or sometimes urinary) amylase activity. It is effectively a diagnosis of exclusion: the finding of a very high serum amylase activity is very suggestive but is not on its own diagnostic
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Enzymatic Activity of Salivary Amylase Abstract: Salivary amylase is an enzyme that can digest starch molecules and break them down to sugar molecules. In this experiment‚ the enzymatic activity and specificity of salivary amylase was examined depending on the changes in pH and temperature. In the first part of the experiment‚ the effect of temperature was determined‚ using constant temperature bath (4‚ room temp‚ 37‚ 50‚ 60‚ and 70°C). Having the room temp and 50°C as the highest and 37°C as infinite
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structure of the enzyme is mainly dependent on the active site and variable groups. Extreme temperatures or extreme pHs can alter the structure of an enzyme. Enzymes function to lower the activation energy to break the bonds. They achieve this by putting stress and pressure on the bonds or creating a microenvironment for the substrate. Enzymes are regulated by inhibitors or activators and can be inhibited by the products of the reaction‚ called feedback inhibition. Enzymes are catalytic proteins;
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VISIBLE SPECTROPHOTOMETER INTRODUCTION In chemistry‚ spectrophotometry is the quantitative measurement of the reflection or transmission properties of a material as a function of wavelength. It is more specific than the general term electromagnetic spectroscopy in that spectrophotometry deals with visible light‚ near-ultraviolet‚ and near-infrared‚ but does not cover time-resolved spectroscopic techniques. Spectrophotometry involves the use of a spectrophotometer. A spectrophotometer is a photometer
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Austin Peay State University Department of Chemistry CHEM 1021 BREAKING DOWN STARCH USING SALIVARY AMYLASE Caution: You will be using a Bunsen burner and glassware to create your own constant water bath. Appropriate caution should be exercised when dealing with the Bunsen burner‚ hot water‚ and glassware. Purpose: Many plants store their energy in the form of starch‚ a polysaccharide made from repeating units of the monosaccharide glucose. Our bodie
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on the activity of the enzyme α- amylase. Introduction Enzymes are a biological catalysts‚ which means that they speed up the chemical reactions in living organisms. Almost all of enzymes are energized protein molecules that catalyse and regulate nearly all biochemical reactions that occur within the human body. The reason in which enzymes are sensitive to heat‚ pH and heavy metal ions is because they are made up of proteins. The food we eat is turned into energy by enzymes and then this energy is
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The role of salivary amylase in the digestion of starches remains controversial. In the absence of pancreatic amylase‚ the key enzyme for starch digestion‚ salivary amylase may well represent a potential compensatory alternate pathway for the digestion of amylose‚ amylopectin‚ and glycogen. Clinically significant depression of pancreatic amylase occurs in chronic pancreatitis‚ pancreatic resection‚ pancreatic neoplasm‚ cystic fibrosis‚ and other causes of pancreatic insufficiency. Quantitation
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Enzymatic Activity of Salivary Amylase Ong‚ Janela Rose I.; Paguia‚ Maria Tricia C.; Placente‚ Dax Daven A.; Posadas‚ Grace Catherine A. 3Bio3-Group 8 Department of Biological Sciences‚ College of Science University of Santo Tomas‚ España‚ Manila 1008 Abstract This experiment aims to examine the enzymatic activity and specificity of salivary amylase depending on the changes in pH and temperature; and determine the optimum temperature and pH of the amylase. EXPERIMENTAL In the
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The Effect Of Enzyme Concentration On Enzyme Activity The pancreatic duct in individuals who have cystic fibrosis frequently becomes blocked‚ reducing or preventing the release of pancreatic enzymes into the small intestine. The aim of this activity is to investigate the effect of a reduction in enzyme concentration on the rate of reaction‚ in this case the breakdown of protein by protease enzymes. Aim – Milk powder contains a white protein called casein. A white suspension of
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