was designed to investigate the effects of temperature on reactions between the enzyme catalase found in animal tissue with the substrate H2O2. The hypothesis stated that an increase in the temperature of the substrate would create a subsequent increase in the rate of reaction between the enzyme and the substrate. This hypothesis was tested by immersing 1cm cubes of animal tissue (sheep liver) which contained the enzyme catalase into the substrate (H2O2 ) mixed with detergent which foamed showing a
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Experimental Analysis on Enzymatic Behavior of Human and Fungal Amylase Lab name and number: Enzymes‚ Lab #5 Panther I.D: 2640403 Shayra Medal Instructor: Emily Nodine Section U21 October 26‚ 2011 X_______________________ Abstract Section The concept of this experiment was to analyze the enzyme Amylase and its environmental behavior. Amylase breaks down the biological macromolecule‚ carbohydrates‚ specifically starch into condensed subunits categorized as monosaccharaides or disaccharides
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ENZYMES LABORATORY REPORT Introduction The utilization of any complex molecule for energy by an organism is dependent on a process called hydrolysis. Hydrolysis breaks complex molecules into simpler molecules using water. Similarly‚ the process that is the reverse of this is called dehydration synthesis‚ which removes water from simpler molecules. However‚ because hydrolysis occurs very slowly‚ living organisms use biochemical’s called enzymes to speed up the reaction. In this lab exercise
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Chavez Period 1‚ Bio Acc. Mrs. Petrov October 1‚ 2014 Tyrosinase Enzyme Lab Question What are enzymes and how can we cause changes in enzyme function? Objective The objective of this experiment is to observe if changes in temperature will cause a disruption in enzyme function. Hypothesis If we add and boil enzyme in L-Dopa‚ then the color will become darker because the temperature will denature some of the enzymes. Variables Independent: Temperature Dependent: Color change Control:
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Virtual Lab: Enzyme Controlled Reactions Worksheet 1. Which of the following does NOT apply to an enzyme: Inorganic 2. When an enzyme catalyzes a reaction: Substrate(s) bind in the active site 3. Which of the following would interfere most with the ability of an enzyme to catalyze a reaction? Reduced concentration of substrate available 4. Feedback mechanisms regulate the rate of enzyme activity‚ effectively “turning off” an enzyme in a
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Enzyme Specificity Using Beta-galactosidase and Alpha-galactosidase Introduction: In order to survive‚ organisms need reactions to occur at a certain time and place. Enzymes make these reactions happen. Enzymes are highly specific causing only one enzyme to catalyze one reaction. “Induced fit” explains why enzymes act this certain way. An enzyme will surround a certain substrate and form to it for the reaction to occur in the active site. If it doesn’t form to it‚ the reaction will not occur
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Effect of temperature on enzyme activity In this experiment… Independent variable: Temperature of the amylase Dependent variable: Enzyme activity which is measured by the time for disappearance of starch Controlled variables: Volume of amylase; volume of starch solution; concentration of amylase; concentration of starch solution Prediction of results i) At low temperature‚ the rate of amylase activity is very low. ii) At optimum (=best) temperature‚ the rate of amylase activity is the highest
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Enzyme application in diary MODIFICATION INDUSTRY PRESENTED BY GROUP H S/NO. NAMES MATRIC NUMBER 99. BASSEY C WINIFRED ENG1102141 100. ABIOLA RAYMOND ENG1106478 101. AMAYO .F. 0SARUWENSE ENG1105472 102. MATH-OSAGIE FRANCIS ENG1102177 103. BETTY .O. UKHUEDUAN ENG1102209 104. IJELI .J. CHUKWUMA ENG1102163 105. EMOGHENE EMUDIAGHA ENG1102151 106. 107. OGBOZOR .J. TOCHUKWU ENG1106008 108. BABAFERI .V. EVUARHERE ENG1102140 109. OTOIDE .O. JOSEPH
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Investigate Concentration of Substrate on Catalase enzyme 1. Aim/Prediction. Aim of this experiment is to investigate concentration of substrate on catalase enzyme‚ as well to see how they work in different concentrations of hydrogen peroxide (H2O2). Prediction would be‚ that the filter paper discs that were used‚ will rise up to the surface in a time in the tube of different concentration of hydrogen peroxide. And in different concentrations the time will be different. As higher the concentration
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Restriction Enzymes Restriction enzymes (also known as restriction endonucleases) are a group of bacterial enzymes which cut double-stranded DNA (dsDNA) into smaller fragments at specific points. They are a defence mechanism used by bacteria to cleave the DNA of invading viruses‚ thereby restricting their expression. The exploitation of restriction enzymes ability to cut large pieces of DNA into smaller fragments (called restriction fragments) and the highly specific way in which they do this
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