abundant long chain macromolecules in all cells and they serve as structure and function matter in cells of all living organisms such as hormones‚ enzymes‚ antibodies. [1] Fig. 1 Fig. 2 General structure of amino acids Protein fragment showing peptide bond Basis of Spectrophotometer Measuring amount of substance in solution by helping of solution color is called colorimetry. Devices that used for this method is called colorimeter
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Lab 4 Quiz 1. Enzymes and what is their function? a. Enzyme: biological catalysts that regulate chemical reactions that occur in cells. Responsible for things such as converting food to energy‚ replace old damaged tissues‚ disposal of cellular waste products. Are responsible for lowering the activation energy. 2. Most enzymes are proteins with three-dimensional shapes determined by their amino acid sequence. 3. Substrate – is a reactant molecule that binds to the highly specific active site
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Enzyme lab report. Introduction. An enzyme is a protein molecule that speeds up the rates of chemical reactions by many folds. They recognize‚ bind‚ and change specific reactants. They do not change thus can catalyze the same reaction again and again. Activation energy also known as an energy barrier is the amount of energy needed in order to begin a chemical reaction. Catecholase catalyzes the reaction rate of catechol oxidation. Catechol is found beneath the skin of many plants such
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the Optimal pH of the Enzyme Peroxidase with the Aid of a Spectrophotometer ABSTRACT The peroxidase enzyme was partially purified‚ and the enzyme activity was calculated with the use of a spectrophotometer‚ demonstrating the effect of pH on peroxidase activity. The objective of this exercise was to determine the ideal pH for peroxidase activity. The data concluded that the optimum pH for peroxidase activity is 5‚ and the “standardized” enzyme volume is 2.8mL.
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Enzyme are a very important component in living organisms. If enzymes did not exist than life itself would not exist (Lab Manual 3 pg. 1). They help in many different ways that are useful to the body of living organisms. Enzyme are used to speed up chemical reactions (Lab Manual 3 pg. 1). Through this process‚ they are considered very unique because they are not altered or consumed within the reaction (Lab Manual 3 pg. 1). This is why enzymes are considered biological catalysts. They also do not
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Title: The Effect of Varying Amounts of Substrate and Enzyme on a Reaction Rate Abstract In living organisms‚ certain reactions must take place rapidly to assist life. This occurs because of enzymes‚ because all reactions would take place too slowly to sustain life (Jacklet‚ 237). Enzymes are large protein molecules that catalyze specific chemical reactions without being used up in the process. Each enzyme has a region on its surface‚ called the active site‚ which recognizes a specific
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Sterling Hayden AP Biology Mrs. Proehl 24 November 2014 I. Title Effect of Differing pH‚ Temperature‚ and Enzyme Concentration on Catalase Reaction Rate II. Introduction Enzymes are used to increase the rate of specific reactions in the body. Catalase‚ a specific enzyme‚ speeds the breakdown of hydrogen peroxide‚ a toxic chemical produced by cells in the body‚ into water and oxygen (Cain and others‚ 2010). The oxygen can be observed as bubbles coming from the reaction site. Catalase is found in many living tissues of organisms
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Enzyme is a type of protein that speed up the rate of reaction of living organisms. Enzyme also acts as a catalyst for specific chemical reaction by converting a specific reactants into a specific product. The enzyme activity will also be affected by temperature and pH value. In this experiment‚ enzyme was placed in carbohydrate solution at different temperature to identify unknown carbohydrate solution. The enzyme that involved in the reaction is salivary amylase. The action of amylase on starch
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The Determination of the Effect of pH on Amylase Activity Grace Chung Abstract: Amylase is an important enzyme in the human body as it allows for the consumption of starch by breaking the polysaccharide down into maltose units. All enzymes‚ including amylase‚ function best at a certain optimal pH. Therefore‚ in this experiment‚ the effect of different pHs on the reaction rate of amylase is studied. It was hypothesized that the amylase-starch reaction would proceed fastest at a pH closest to
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The purpose of the experiment was to find out how temperature affects the enzymes activity. For example‚ in Humans if the temperature is too high‚ the individual’s brain enzymes can denature and cause life threatening problems. The opposite can occur as well‚ if the temperature is too low‚ hypothermia can occur and it can be dangerous (Wilson‚ 1996). In the experiment optimal conditions for fungal and bacterial amylase was measured as well. Discovering information‚ such as the optimal temperature
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