change with varying degrees of temperature and light exposure? To test this algae beads were exposed to a CO2 indicator‚ which changes colors based on the level CO2 level present. The algae beads were exposed to two different variables‚ light and temperature and tested for color change‚ Ph‚ level‚ and CO2 absorbance level. The beads were exposed to a light and a dark condition‚ and another set was exposed to ice and a warm water bath. It was found that the rate of change of CO2 decreased in the dark
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2011 2. Introduction: Each student was given unknown bacteria and was instructed to perform a variety of experimental tests that would help to identify their bacteria. During the process of identification‚ the unknown bacteria was added to many different testing medias using aseptic technique. They are as follows: lactose fermentation on eosin methylene blue (EMB)‚ TSI (Triple Sugar Iron agar)‚ Phenol red sucrose‚ the SIM test‚ H2S by SIM‚ IMViC (indole‚ motility‚ voges-proskauer‚ and citrate)
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when the enzyme and buffer in the assay are held constant were experimented. We analyzed the change in absorbencies over time for varying substrate concentrations. There were four experimental assays which contained 1% enzyme solution‚ substrate solution of 0%‚ 1%‚ 2%‚ and 3% concentrations‚ guaiacol‚ and pH 7 buffer. At 2% concentration there was a greater enzymatic activity and at 3% concentration enzymatic activity decreased. The results were supported of our hypothesis. INTRODUCTION Enzymes are proteins
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this experiment‚ students should be able to: 1. determine the concentration of coloured solution such as FeCl3 by using dilution and colour differentiating (colorimetric) techniques. 2.2: INTRODUCTION Concentration can be expressed in many different ways such as percentage volume and percentage weight etc. In laboratory‚ normally concentrations were expressed as molarity and normality. Molarity is the mole of material in 1000 mL (1 liter or 1 dm3) of solution‚ as shown as followed equation:
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the acyl-enzyme is then hydrolysed by nucleophilic attack of serine on the peptide carbonyl group‚ collapse of the tetrahedral intermediate and the release of the amine component. Histidine 57 then acts as a general acid catalyst and draws a proton away from the water molecule. The OH- ion then attacks the carbonyl carbon atom of the acyl group which forms another tetrahedral intermediate which breaks down to form the carboxylic acid product. The release of this acid then frees up the enzyme to continue
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Lauren Sullivan Plants Imperfections Abstract The purpose of this experiment was to plant different crosses and observe the different phenotypic ratios the plants present. The procedure of this experiment was plant six different crosses and water them correctly so that we could observe the different phenotypes and compare them to Mendel’s proposed ratios. Mendel‚ who had studied peas‚ did a similar experiment and came up with specific ratios that a monohybrid and dihybrid cross should show. His
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General Biology I‚ Fall 2011 Lab Report 3 Name: Luan Nguyen Date: 11-30-11 Lab: Gel Electrophoresis Purpose of this lab: In this laboratory investigation‚ students will analyze hypothetical human DNA using restriction endonucleases and gel electrophoresis to match samples from a crime scene to a suspect. Introduction: Your analysis unit will use gel electrophoresis to determine the guilty party. Electrophoresis is a technology in science that allows an individual to separate
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Abstract……………………………………………………………………………………………2 Introduction………………………………………………………………………………………..2 Background………………………………………………………………………………..2 Objectives…………………………………………………………………………………2 Scope………………………………………………………………………………………3 Theory review……………………………………………………………………………………..3 Design of report…………………………………………………………………………………...5 Procedures…………………………………………………………………………………………5 Results……………………………………………………………………………………………..6 Discussion…………………………………………………………………………………………6 Conclusion………………………………………………………………………………………...7 Reference………………………………………………………………………………………
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Sp2012 (Set all of your margins to ½”) Purpose: The purpose of this lab is to help you become a little familiar with some of the tests that can be typically performed in a clinical or research lab facility. These tests may help in determining a particular pathogen’s growth needs. There are several sections to this lab. Find each section and complete the “Preparing for Class” sections. Preparing for class - Day 1 Read in your lab manual about the following agar mediums: Blood Agar (pg 168)‚ EMB
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into light (Branchini‚ 2008). In the fireflies case the reaction involves the enzyme luciferase which lights up a lantern-like mechanism under the tip the wings and attached to the end of the body of the firefly. It is presumed that the firefly uses the lantern to attract a mate. The goal in this experiment was to test the effect of adenosine triphosphate in lighting the firefly’s lantern in temperature effects‚ pH effects‚ and salt effect. Materials and Methods Powdered firefly lanterns
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