MISEP Chemistry 512 – Jacobs Enzyme Catalyst Lab - Formal Report – August 8‚ 2007 ABSTRACT This investigation examined what would happen to the rate of an enzyme-catalyzed reaction if the concentration of substrate changed. We hypothesized that if the concentration increased‚ then the reaction rate would also increase. To test our question‚ we varied a combination of substrate and buffer‚ totaling 6mL‚ with a constant amount of 2 drops of catalyst. The enzyme catalyst‚ peroxidase‚ increased
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Lab 6: Enzymes . header Purpose: The purpose of this lab is to test for enzyme activity‚ look at enzyme specificity‚ and how temperature affects enzyme activity. Time need to perform this lab: approximately 3 hours Preparation FIRST: Read the lab in its entirety TWICE before you begin. You will perform the experiment‚ write your lab report and include the answers to the additional 4 questions within the text for full credit on this experiment. Materials: •3% hydrogen peroxide •a
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The enzyme tyrosinase was successively extracted by combining a homogenate of a potato and sodium sulfate with ammonium sulfate. Tyrosinase was successfully extracted by taking advantage of solubility properties of certain proteins. A standard curve was generated indicating dopachrome absorbance values through the use of a spectrophotometer and a computer graphing program. A spectrophotometer was used to measure either the amount of light that passed through a solution (transmittance) of the amount
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Enzyme Lab Marcus James. December 20‚ 2013 HL Biology 3 Period 1 Purpose and Background The purpose of this lab is to explain how enzymes act as catalysts for biological reactions in different temperatures. This lab relates to enzymes‚ proteins‚ and substrates; that we learned in class. The union of the enzyme and the substrate is called the enzyme-substrate complex. The make-up of an enzyme is proteins and made up of chains and amino acids. Enzymes
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Lab 6.C Hypothesis: If enzyme activity is affected by the pH of a solution‚ then the enzymes will experience the greatest activity at a pH of 6. Variables: Independent Variables Dependent Variable Controls Four different pH values (10‚ 7‚ 6‚ and 3) Change in color of the solution The amount of potato extract‚ pH solution‚ and catechol used (1 cm +/- .1cm) Size of the test tubes Amount of time allowed for the catechol to sit with the potato extract and pH solution (20 minutes with 5
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Potato Enzyme Lab INTRODUCTION An enzyme is a protein that speeds up or slows down a specific chemical reaction in an organism. A good rule of thumb is to remember that enzyme names end in “-ase”. This will help in identifying enzymes in further readings. Generally enzymes are catalysts. Hydrogen peroxide is a toxic chemical that is produced in many organisms during metabolism. Organisms must get rid of this toxin to survive. One reaction turns the hydrogen peroxide into water and oxygen. The
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Enzyme Lab 6 03/13/2013 Report by Mary Jo Anthony I. Introduction II. Materials and Methods III. Results IV. Conclusion and Discussion Introduction Background Information: This lab allowed us to study chemical reactions and how catalysts will affect the rate of these reactions. The reaction we studied is the breakdown of hydrogen peroxide to water and oxygen and it is vital to life. The molecule hydrogen peroxide is a molecule that is toxic
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Enzyme Lab: Peter Kuetzing – 10/4/2012 – F Block 1. How does changing the concentration of enzyme affect the rate of decomposition of H2O2? When more enzymes is added the rate of reaction speeds up. In this case‚ H2O2 will produce more O2‚ in return the kpa/min will go up. 2. What do you think will happen to the rate of reaction if the concentration of enzyme is increased to five drops? Predict what the rate would be for 5 drops. I think that the rate of reaction will slightly increase from
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A Quantitative Enzyme Study: CATALASE FlowChart Purpose: Measure the rate of enzyme activity in different conditions Procedure: A. Extraction of Catalase 1. Peel potato 2. Cut into cubes 3. Mass 50g 4. Measure out 50ml of cold distilled water in blender 5. Add crushed ice into blender (small amount) 6. Add the potato cubes into the blender 7. Turn on blender on high for 30 seconds 8. Prepare an ice bath - FROM THIS POINT ON PREPERATION MUST BE CARRIED OUT IN AN ICE BATH – 9
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Enzymes Abstract: The following 2 labs experimented the more enzymes and substrates added to the concentration will effect the reaction rate. Our second lab‚ we tested enzyme and substrate concentrations to determine the increase of temperature and inhibitor. The enzyme source used in both labs was peroxide‚ guaiacol is used as a substrate for peroxide. We used Guaiacol‚ turnip extract‚ peroxide and distilled water for enzyme and substrate concentration. In the second lab we used the same substances
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