results. This is due to the enzymes being most active at 60ᵒC‚ it was that results were going to be most active at the 41ᵒC which is close to human body temperature. When we compare the enzyme in the low temperatures to the enzymes in the higher temperature we observed that the enzymes were functioning at a significantly reduced rate at the 24ᵒC temperature and was completely inactive at the 0ᵒC temperature. Whereas when we compare to the higher temperatures (60ᵒC) the enzymes should have functioned
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EXPERIMENT TO SHOW THE EFFECT OF TEMPERATURE ON ENZYME ACTIVITY Table of raw data of experiment to show effect of temperature on enzyme activity Temperature (ᵒc) (+/-0.05) | Time taken for pink solution to turn back to white (s) (+/- 0.05) | | Repeat1 | Repeat2 | Repeat3 | Repeat4 | Repeat5 | 21 (Room Temperature) | 414.0 | 286.0 | 286.0 | 297.0 | 294.0 | 40 | 122.0 | 88.0 | 125.0 | 86.0 | 92.0 | 60 | 176.0 | 162.0 | 161.0 | 166.0 | 180.0 | 80 | 356.0 | 367.0 | 396.0 | 85.0 | 438
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Writing of Lab Reports __________________________________________________________________________ W hy should I bother writing lab reports in the correct way?” The Foundation Programme is designed to prepare you for undergraduate studies at UTAR which will require the writing of lab reports all years generally. At the end of your third year‚ you may have an opportunity to work on scientific projects which will culminate in an official scientific report. Depending on the quality of your report
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What are Enzymes? Enzymes are proteins that quicken chemical reaction in our body. They are found in our digestive system‚ in the stomach mostly. They are there to speed up the process of breaking down food into molecules that provide nutrients to the body. Enzymes are made up of amino acids. The Digestive System: Many people believe that when you eat a meal it drops into a pool of stomach acid‚ and then it is broken down as it goes into the small intestine to have nutrients taken out‚ and then
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Scientific Writing and Standard Components of a Lab Report INTRODUCTION Writing is a powerful tool. Effective communication is necessary to succeed in any field. As a professional‚ you must communicate with both your colleagues‚ and the community at large. Writing is the means of showing the value of your work. The specific style of writing in science is based on the unique audience of readers. In addition‚ writing allows one to organize one’s thoughts and clarify ideas‚ and it fosters abstract
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Abstract: Enzymes help determine a phenotype through proteins. The experiment presented tests how enzymes affect the pigment in the eye of Drosophila. What was resulted in this experiment is that when certain enzymes change‚ a mutation is created‚ causing the change of pigments in the different Drosophila’s eyes. The production of one enzyme is one gene and a mutation of this gene can cause an enzyme to be inactive which leads to the phenotype changing. Introduction: George W. Beadle and Edward L
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the Optimal pH of the Enzyme Peroxidase with the Aid of a Spectrophotometer ABSTRACT The peroxidase enzyme was partially purified‚ and the enzyme activity was calculated with the use of a spectrophotometer‚ demonstrating the effect of pH on peroxidase activity. The objective of this exercise was to determine the ideal pH for peroxidase activity. The data concluded that the optimum pH for peroxidase activity is 5‚ and the “standardized” enzyme volume is 2.8mL.
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inside of the amylose coil.The amount of blue complex that starch gives with iodine can be measured by using a spectrophotometer. α-amylases are found in saliva‚ pancreatic juice‚ human breast milk‚ serum and certain tissues such as the liver. This enzyme catalyzes the hydrolysis of α (1-4) linkages in starch by breaking it down to maltose and some glucose. As the starch is broken down‚ the coiled structure of α-amylase is unfolded. Therefore‚ iodine will no longer be able to form the blue complex
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the acyl-enzyme is then hydrolysed by nucleophilic attack of serine on the peptide carbonyl group‚ collapse of the tetrahedral intermediate and the release of the amine component. Histidine 57 then acts as a general acid catalyst and draws a proton away from the water molecule. The OH- ion then attacks the carbonyl carbon atom of the acyl group which forms another tetrahedral intermediate which breaks down to form the carboxylic acid product. The release of this acid then frees up the enzyme to continue
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Investigating the effect of pH on the activity of the enzyme catalase. Introduction Hydrogen peroxide (H2O2) is a very pale blue liquid which appears colourless in a dilute solution‚ slightly more viscous than water. It is a weak acid. It has strong oxidizing properties and is therefore a powerful bleaching agent that is mostly used for bleaching paper. Catalase is a common enzyme found in all living organisms. Its functions include the conversion of Hydrogen Peroxide‚ a powerful and potentially
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