Biofuel Enzyme Kit Katie Adamson Biochemistry Laboratory‚ BIO124L 1/29/15 Abstract The objective in this lab was to determine the effects different conditions had on the enzyme cellobiase. We examined reaction rates in the presence or absence of an enzyme‚ the effects temperature and pH changes on the enzyme and the effects enzyme concentration and substrate concentration had on the enzyme. As expected results showed us that cellobiase works optimally when conditions are favorable. We see this
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Enzyme Lab Experiments Problem: How can we demonstrate how enzymes work? What happens if we alter the environment of an enzyme? Materials: G;lucose Test Strips Test Tubes Pipettes Raw Hamburg Lettuce Potato Raw Liver Chalk Beakers Dairy Lactose Tablet Water Sugar Solo Cups Hot Plate Knife Gloves Skim Milk Glow Sticks Peroxide Hypothesis: 1. If we change the environment via temperature the glow stick will Its intensity will change 2. If hydrogen peroxide is added to a certain food liver
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Enzymes INTRODUCTION Enzymes are protein cells and they are found in the gut. They help to break down the food you eat. Enzymes are produced by living cells and they speed up reactions because they are biological catalysts. If we did not have enzymes‚ the reactions in our cells would be too slow to keep us alive. This means that they are extremely important for our survival. The properties of enzymes are: ⋆ They can be used many times‚ because they can speed up reactions without
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Environmental Parameters of Enzyme Activity Alex Rocha Texas State University Abstract If you’ve ever left a cut up apple out for long‚ you’ll notice that after a while‚ it will turn brown. The reason for this is an enzyme named catechol oxidase‚ a ubiquitous plant enzymes containing a dinuclear copper center (Klabunde‚ Eicken‚ Sacchettini‚ & Krebs‚ 1998). In this experiment‚ we used two different chelators‚ ethylene diamine tetraacetic acid and phenylthiourea to test which would stop
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experiment diluted solutions of amylase were created and then tested using a starch solution‚ I2KI for reaction times. The answer to the question was proved to be that more concentration of amylase speeds up the reaction time. Introduction The enzyme‚ amylase is found in the saliva of most animals and in humans. Amylase hydrolyzes starch‚ a plant’s reservation of carbohydrates. Amylase causes a chemical reaction in the polysaccharide starch that breaks down the glucose molecules into maltose
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Dana Calderone Responses of Enzyme Activity from pH and Concentration Abstract Enzymes are the key to many of the chemical reactions that our bodies depend on to live. Without enzymes‚ we would not exist. These biological catalysts speed up the reactions as well as reduce the amount of activation energy needed to complete the process. Knowing how important enzymes are to us‚ it is important to realize what they require to function. They need select conditions and rates to work right. These conditions
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Enzymes and ATP Enzymes act as protein catalysts in biochemical processes Enzymes bind to a substrate and forms the enzyme substrate complex. Enzymes work by lowering the energy of activation. Activation energy must be supplied for the reaction to begin‚ once supplied‚ the reaction can proceed on its own. Enzymes can speed up events. They are not used by during the reaction because the enzyme stays the same‚ it does not change during the reaction. (Hudon-Miller‚ Enzymes‚ 2013) Enzymes act as
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temperature on the activity of lipase Aim: To investigate the effects of temperature on the activity of lipase enzyme on milk which contain fats or lipids. Introduction: Enzyme is a kind of biological catalyst that made up of protein. It can speed up the metabolic reactions on various kinds of substances. Like lipase can break down lipid into glycerol or fatty acids in milk. Since enzyme is made up of protein which easily affected by varies temperature. This experiment is carried out to find the
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Enzymes What Are Enzymes? Substances that speed up chemical reactions are called catalysts. Organic catalysts are called enzymes. Enzymes are specific for one particular reaction or group of related reactions. Many reactions cannot occur without the correct enzyme present. They are often named by adding "ase" to the name of the substrate. Example: Dehydrogenases are enzymes that remove hydrogen. Induced-fit Theory The shape of the enzyme must match the shape of the substrate
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Enzyme action is the simple mechanism by which enzymes catalyse chemical reactions. This begins with the binding of the substrate to the active site on the enzyme. The binding of the substrate to the enzyme causes changes in the distribution of electrons in the chemical bonds of the substrate. This then causes the reactions that lead to the formation of products that are then released from the enzyme surface to regenerate the enzyme for another reaction cycle. The active site has a unique shape that
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