RUNNING HEAD: Understanding Enzymes Understanding Enzymes Amanda Chung Blanche Ely High School Abstract The researcher conducted this experiment in order to investigate how various factors effected enzymes. Those factors were the concentration of the enzyme‚ the temperature it had to work in‚ and the pH level of the solutions as well. All three were hypothesized to speed up the rate at which the enzyme acted. A series of several tests were carried out to find the answer to each problem
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Title: “The Effect of Substrate Concentration‚ Enzyme Concentration‚ pH and Temperature on Enzyme Activity” Abstract: In the following experiments we will measure precise amounts of potato extract as well as Phenylthiourea‚ combined with or without deionized water and in some instances change the temperature and observe and record the reaction. We will also investigate the different levels of prepared pH on varying samples of the potato extract and the Phenylthiourea and record the results.
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Lab Report Effect of temperature on enzyme activity I. Purpose: Determine how effect of temperature on enzyme (catalyse) activity (in the liver). II. Materials * Raw liver * Forceps * 50mL 1% hydrogen Peroxide (H * 25mL graduated cylinder * 50mL‚ 400mL beakers * Pureed liver * 5 Filter-paper disks * Paper towels * Timer or stopwatch * Thermometer * Hot plate III. Procedure 1.Gather all necessary materials; start
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Effect of pH on Enzyme Activity A piece of Solanum tuberosum (potato) was removed and mixed with distilled water in a blender. The resulting solution was filtered through multiple layers of cheese cloth to filter out the liquid by eliminating any large pieces in the solution. The solution created was catechol. Five different solutions were prepared as blanks with each test tube containing 6.0mL of a different pH (pH 4‚ pH6‚ pH7‚ pH8‚ pH10) of phosphate buffer‚ 1.0mL of the enzyme and 1.0mL of water
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pH on Enzyme Activity Athenaa Jeyachandran Mr.Menes Course Code January 21st‚ 2013 The Effect on Enzyme Activity Due to Temperatures Purpose: The purpose of this lab experiment is to examine how different temperatures affect enzyme activity using hydrogen peroxide. Hypothesis: My hypothesis for this experiment is that temperatures near body temperature is when enzyme activity will be at its highest. I believe this will occur because in our body‚ enzymes are very
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10.10.2012 Internal assessment on enzyme concentration Enzymes are proteins that act as catalysts and they are the most important type of protein. My aim in this experiment was to investigate the effect of enzyme concentration on the rate of an enzyme (protease) – catalyzed reaction ‚ catalysts speed up chemical reactions . My hypothesis is that the rate as reaction will increase as the
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This experiment is to determine the unknown DNA plasmid using restriction enzymes and conducting electrophoresis finally comparing the resulting fragments with the known restriction map. In this lab‚ it succeeds in showing the fragments. In this report we will discuss the‚ results‚ limitations and possible errors. Introduction In biology restriction enzymes are used in several ways to modify and manipulate DNA molecules. One common use is to compare pieces of DNA from one that is unknown‚ with fragments
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The objectives of this experiment were to investigate the activity of enzymes‚ components that influence the enzyme’s activity‚ identify an unknown phosphatase‚ influence of inhibitors‚ and determine if inhibition is competitive or noncompetitive. A spectrophotometer evaluated the measurement of color change over a period time due to product being formed. Determining unknown phosphatase and effects from different inhibitors were determined by varying the pH and substrate concentrations. The unknown
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and measure the enzyme activity of β-galactosidase in the different concentrations of o-Nitrophenylgalactoside (ONPG) using a spectrophotometer. The spectrophotometer was also set at 420nm‚ a wavelength which is best for recording the absorbance values for the experiment. From the results‚ 0.9mM ONPG solution has the highest absorbance and 0.1mM ONPG solution has the least. Also‚ 0.5mM ONPG solution has the highest rate of enzyme activity and it is the most efficient as the enzyme activity of the
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Enzymes: Food & Nutrition What are enzymes Enzymes are a type of protein produced by a living organism used to catalyze chemical reactions in cells. These reactions allow the cell to build things or take things apart as needed in order to grow and reproduce. How do enzymes work - in steps 1) Substrate floats near enzyme 2) Substrate and enzyme connect – which breaks it into products 3) Products are released ex) BreadFast & Co.’s use of enzymes The company uses many different
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