Background Information Part 1 In the first part of the enzyme lab‚ we mixed a substrate and an indicator with an enzyme. There was also a neutral buffer in each of the chemical mixtures. The neutral buffer regulated the pH to around 7. We got a color palette and once we mixed each together‚ we observed and saw a change in the color of the substance. The darker and more brown the substance got‚ the more oxygen produced by the reaction. Our results showed that amount of oxygen produced increased
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Determination of the Activation Energy of an Enzyme Catalysed Reaction PRACTICAL Report & Criterion Reference Grid * The expected sequence and details required for your practical report are outlined below. Please follow them closely‚ as they will facilitate the production of a structured report. * An introduction giving a synopsis of the experiment and the importance of rates of reaction and activation energy. This section should also state the aim of the practical (0.50-page) – 5 marks
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Sang Kim Enzyme Catalyst Purpose/Problem: There are four parts to the Enzyme Catalyst lab - Activity A‚ B‚ C‚ and D. In activity A‚ the characteristics of enzyme actions will be observed. The main purposes are to determine the rate of an enzyme catalyzed reaction‚ to study the characteristics of an enzyme mediated reaction‚ and to observe the effect of heat on enzyme activity. The purpose of activity B is to use the Titration Protocol to determine the initial amount of H 2O2 present
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Enzyme Lab Daniyal Abdali (Rachel Lee) (Sarina Dolch) SBI 3UI Mr. Vrabec October 20‚ 2009 Test #1 * Add a small piece of cracker in test tube #1 and add Lugol’s solution. Observation #1 * The cracker turned a black colour when the Lugol’s solution was added to it. This was a positive result‚ meaning that the cracker contains starch. Test #2 * Add a bigger piece of cracker in test tube #2‚ add 5 mL of Benedict’s solution‚ place in a boiling water bath‚ and record observations
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Purpose: The purpose of this lab was to test how objects slightly acidic or slightly basic will affect the way catalase‚ the enzyme tested‚ denatures‚ or breaks down. Hypothesis: If the potato is acidic‚ it will react with the H2O2 more than it will with the raw‚ plain potato because the acid will denature the enzyme faster. The manipulated/independent variable is the raw‚ plain potato while the responding/dependent variable is the other types of potatoes used in the experiment. Materials:
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Effects of Temperature‚ pH‚ Enzyme Concentration‚ and Substrate Concentration on Enzymatic Activity INTRODUCTION Enzymes‚ proteins that act as catalysts‚ are the most important type of protein[1]. Catalysts speed up chemical reactions and can go without being used up or changed [3] Without enzymes‚ the biochemical reactions that take place will react too slowly to keep up with the metabolic needs and the life functions of organisms. Catecholase is a reaction between oxygen and catechol
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phosphate (a synthetic substrate) at an optimum pH of 10.0 with the liberation of p-nitrophenol. The substrate is colourless‚ but the product p-nitrophenol is yellow in alkaline solution‚ absorbing maximally at 405 nrn. Thus a convenient assay for this enzyme involves monitoring the change in absorbance of the reaction medium at 405 nm. Exergonic (i.e energy producing reactions) exhibit a negative free energy change. Sometimes these reactions occur spontaneously‚ but generally some energy must be supplied
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Enzymatic activities of bioactive washing powder Title: Investigation of the amalyse activity f bioactive washing powder Objective: To investigate the amalyse activity of the two brands of bioactive washing powder – “Super clean” and “Magic power”. Principal: Amalyse can catalyse the breakdown of starch into maltose. In this practical‚ solutions of the 2 washing powders will be filled into 2 identical wells on the starch agar plate separately. Starch will be broken down by the amylase disused
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Enzymes are biological molecules (proteins) that act as catalysts and help complex reactions occur everywhere in life. Enzymes are catalyst for biochemical reactions; Enzymes lower the activation ATP so a reaction can begin over the energy barriers. In this lab‚ a discussion of the enzymes reaction to heat will be addressed. Does heat speed up the enzyme reaction? The prediction is as more heat is applied more reactions will occur then at some point the heat will denature the enzyme as it reaches
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inside of the amylose coil.The amount of blue complex that starch gives with iodine can be measured by using a spectrophotometer. α-amylases are found in saliva‚ pancreatic juice‚ human breast milk‚ serum and certain tissues such as the liver. This enzyme catalyzes the hydrolysis of α (1-4) linkages in starch by breaking it down to maltose and some glucose. As the starch is broken down‚ the coiled structure of α-amylase is unfolded. Therefore‚ iodine will no longer be able to form the blue complex
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