________________________________________________ Introduction: What was your null hypothesis? be specific A change in temperature in the reaction of catechol and catecholase will not change the absorbance of reaction over time. What was your alternative hypothesis? be specific As the environment and temperature is changed from 0 °c to 20°c to 95°c‚ the absorbance of catechol and catecholase reaction will increase over time. The rate of reaction increases as the temperature increases. Results: What were the main trends
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Act Practice Test SCIENCE TEST 35 Minutes—40 Questions DIRECTIONS: There are seven passages in this test. Each passage is followed by several questions. After reading a passage‚ choose the best answer to each question and fill in the corresponding oval on your answer document. You may refer to the passages as often as necessary. You are NOT permitted to use a calculator on this test. Passage I Metamorphic rocks form when temperature and/or pressure cause changes in preexisting
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Performed: April 30‚ 2013 Date Submitted: May 10‚ 2013 Experiment # 7 Enzyme Activity Objectives: 1. Associate the presence of enzymes with the catalysts of the chemical reaction in living cells. 2. Determine the effect of enzyme concentration‚ substrate concentration‚ temperature‚ pH and heavy-metal salts upon the activity of salivary amylase. Results: A. Enzyme Concentration Test tube | Drops of Enzyme | A-1 Starting Time | A-2Time Blue Color Fades | A-3Time for Starch Hydrolysis
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and Irish Potato Hypothesis: Polyphenol Oxidase enzyme activity can be detected by change in colour of solution‚ Inhibitors prevent the reaction of the enzymes with substrates‚ the enzyme is relatively specific. Aim: To design and conduct an experiment to demonstrate enzyme activity of Polyphenol Oxidase and Peroxidase when mixed with catechol‚ caffeic acid‚ pyrogallad‚ tyrosine‚ guacol‚ and water‚ to test the effect of inhibitors on these enzymes‚ to show the specificity of Polyphenol Oxidase and
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During this phase‚ RNA synthesis‚ production of ribosomes‚ enzymes and other molecules occur (Mullenger‚ 1973). This is followed by the exponential
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Fungi Objective : To screen for thermophilic fungi as producer of fungal cellulase. Introduction One of the most important sources of carbon that is abundantly found on this planet is cellulose. While cellulase is the enzyme to degrade this carbon and it is a key enzyme in the bio refinery process of producing green chemicals (Ponnambalam et al.‚ 2011). In screening for the cellulolytic fungi‚ several qualitative display of cellulolytic such congo red clearing zone assay‚ gel diffusion assay
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INTRODUCTION Apples contain an enzyme called polyphenol oxidase (Phenolase). Phenolase has catecholase and cresolse activity‚ also containing small traces of copper. When apples are cut‚ phenolase is released from the cells and is exposed to oxygen. When phenolase reacts with oxygen‚ it catalyses one step of the biochemical conversion of phenolic compounds. The presence of oxygen hyrdroxylates the colourless phenols‚ which are subsequently oxidised to coloured quinones known as melanin. (Vlasta
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The dialysed enzyme was loaded onto a column of Sephadex G-50 (120 cm × 1.0 cm) equilibrated with 10 mM Tris-HCl buffer‚ pH 8. The column was eluted at a flow rate of 1 ml / 6 min. The elution profile of gel filtration chromatography is shown in the (Fig: 1). The fractions collected were determined for its total protein concentration and fibrinolytic enzyme activity. The active fraction obtained was pooled together‚ concentrated by lyophilization and used as purified fibrinolytic enzyme for subsequent
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a sodium chloride (NaCl) solution‚ subjecting the product to detergent solution (dH2O)‚ filtering the solution and lastly‚ the addition of ethanol. When beginning with a solid substance‚ such as a banana‚ crushing the substance allows for breaking down the cell membrane and cell wall‚ if using a plant cell. This ultimately‚ removes the contents of the cell from the deoxyribonucleic acid (DNA) and further exposes it for the detergent solution. After this step‚ one would use NaCl solution to stabilize
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DIFFUSION= The movement of atoms or molecules from an area of higher concentration to an area of lower concentration. Atoms and small molecules can move across a cell membrane by diffusion. OSMOSIS= Diffusion of fluid through a semipermeable membrane from a solution with a low solute concentration to a solution with a higher solute concentration until there is an equal concentration of fluid on both sides of the membrane. SELECTIVE PERMEABLE MEMBRANE=a membrane (as a cell membrane) that allows
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