Benjamin Tiger1/16/12 Cellular Respiration Questions 1. Aerobic pathways require oxygen‚ while anaerobic pathways don’t. Anaerobic pathways only require the process of glycolysis to produce energy. Anaerobic pathways are found outside the mitochondria within the cytoplasm of the cell‚ with a low efficiency of 4%. These pathways require glucose‚ ATP‚ adolase‚ fructokinase‚ dehydrogenase‚ and NAD+. Out of one glucose molecule‚ major products include two net ATP‚ two NADH‚ and two pyruvate
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in which yeast is tested with anaerobic conditions and the amount of time it would take to get to the result of fermentation with different sugars. This fermentation rate was calculated with water displacement using pipettes to discover the span of time before the release of air bubbles known as Carbon Dioxide. Sucrose had the highest fermentation rate in comparison to all of the other sugars that were tested. The results confirm the capability of cells when it comes to cellular respiration despite
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Cellular Respiration Lab Report Wednesday 8:00 A.M. Lab March 2nd‚ 2005 I. Introduction In this lab we are measuring the amount of oxygen used in both germinating and non germinating peas. We are measuring the oxygen consumption by taking a reading of a respirometer submerged in two water baths. The first bath will be cold water and the second warm to determine the effect of temperatures on oxygen consumption. Our negative control will be glass beads to measure to increase or decrease
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Enzyme Report Case 1 - Hereditary Fructose Intolerance 1 & 2. Enzymes take on a variety of roles in the human body at the cellular level. Specifically‚ they aid in the breakdown of macronutrients such as glucose and fructose so that the body can use them. Although reactions within the body would occur without enzymes‚ enzymes provide control because the reaction is not needed all of the time. Enzymes function in specific environments such as temperature or pH; some enzymes are specific
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My lab group studied the effect of PH on reaction rate/ enzyme activity measured by foam height. PH is the measure of the concentration of hydrogen ions in a solution. The higher the hydrogen ion concentration‚ the lower the pH. Every enzyme has an optimal PH‚ meaning they have a very small window in which they are most active. Our enzyme (potato smoothie) had an optimal PH of 7.0-7.5. We know this because we measured the enzyme’s reaction rate by measuring foam height. The largest foam height we
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The methylene blue staining procedure is used to measure yeast viability based on the assumption that the methylene blue will enter the cells and be broken down by living yeast cells that produce the enzymes which breaks down methylene blue‚ leaving the cells colourless. The non- viable cells do not produce this enzyme (or enzymes) and as such the methylene blue that enters the cells are undegraded causing the cells to remain coloured (the oxidized form concentrates intracellularly). The coloured
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Fermentation Lab Report Introduction: Fermentation‚ a type of anaerobic respiration that breaks down glucose into ethanol and carbon dioxide without the use of oxygen‚ is extremely vital in food processing. Especially useful in the making of bread and wine is yeast‚ a single-celled fungus. The rate of fermentation of these products can be done by measuring the amount of carbon dioxide produced by the work of the yeast. The specific variable we tested was the volume of fructose in each vial solution
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Effect of the Changes in the Environment to the Functionality of the Enzymes Introduction a. Background In our everyday lives‚ enzymes are used in our bodies‚ and in nature around us‚ to speed up the chemical reactions happening constantly‚ which happens by lowering the amount of activation energy needed to start various reactions. The way this works is by attaching the particular substrate to the active site of the enzyme‚ where it will start to aid the chemical reaction. Then‚ the allosteric
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Experiment 4: Enzyme Kinetics. Results/Discussion Week 1 Part A: Table 1. Enzyme activity for each assay of 4-nitroaniline formation. Rate of 4-nitroaniline formation Name of trial Abs/sec Abs/min M/min mol/min µmol/min #1 0.00003 0.0018 2.05x10-7 2.15 x10-10 2.15 x10-4 # 2 0.00010 0.0060 6.81x10-7 7.15x10-10 7.15x10-4 # 3 0.00020 0.0120 1.36x10-6 1.43x10-9 1.43x10-3 # 4 0.00030 0.0180 2.00x10-6 2.10x10-9 2.10x10-3
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Cellular respiration‚ which synthesis ATP‚ begins with glycolysis‚ wherein a six-carbon glucose is broken down into two three-carbon molecules called pyruvate. This process requires the input of two ATPs to produce two pyruvates‚ two NADHs‚ and 4 ATPs. The NADHs are synthesised when NAD+‚ delivered by B vitamins‚ become bound to hydrogen and energised electrons1. Following glycolysis is the Krebs cycle and electron transport chain respectively. The Krebs cycle uses the two pyruvates produced in glycolysis
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