Matthew Saldanha Bio DCP lab-Catalase experiment Aim: To investigate enzyme kinetics‚ using different concentration of the enzyme. Hypothesis: The assay system used in the lab consists of a filter paper disc coated with the enzyme and the dropped into a papercup of substrate (Hydrogen Peroxide). As the hydrogen breaks down the hydrogen peroxide into hydrogen and oxygen gas‚ the bubbles of oxygen gas collect underneath the filter and make it
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Enzyme Activity Over Different Concentrations and Effects The goals of this experiment were to examine the effectively of enzymes on samples of different enzyme concentrations and substrate concentrations. In addition‚ the experiment tested how effective enzymes are on samples of pH levels and temperature levels. A. Effect of Enzyme Concentration Hypothesis: With half as much enzyme concentration then the reaction rate will be half as much than when the enzyme concentration is equal
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The Effect of Concentration‚ pH and Temperature on Enzyme Activity Biology For Majors October 4‚ 2012 Abstract We examined the reaction an enzyme has when its concentration‚ pH and temperature are altered. In order to do this‚ we added different levels of pH into different test tubes with the enzyme (sucrose)‚ and substrate (sucrose)‚ and we then inverted the tube. The higher pH produced more enzyme activity. Temperature effects enzyme activity by decreasing its stability when the temperature
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Lab Report (Effect of concentration on enzyme activity) Biology Noor Alawadhi 11- KC Introduction: An Enzyme is a protein‚ which is capable of starting a chemical reaction‚ which involves the formation or breakage of chemical bonds. A substrate is the surface or material on or from which an organism lives‚ grows‚ or obtains its nourishment. In this case it is hydrogen peroxide. This lab report will be explaining the experiment held to understand the effects of the changes in the amount of
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oD 4.1 Respiration Why organisms undergo respiration What is respiration and why do we need it? Respiration is the process whereby energy is released from food molecules (most usually glucose). It takes place inside living cells‚ both eukaryotic and prokaryotic. The process takes place inside the cytoplasm and the mitochondria. Energy is used to synthesise adenosine triphosphate (ATP)‚ which acts as a short-term energy store in cells. All living organisms need energy to drive their
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In the yeast discovery lab we had to decided what the outcome would be then perform the experiment. The experiment was done during class time‚ so everyone’s results would be the same. There were four bottles with warm water in them and to those bottles were added yeast. Then to one of each bottle there was added sugar‚ corn syrup‚ corn starch. To the fourth bottle there was only yeast added and used as a control group. Balloons were then stretched onto the top of the bottles to catch any gas the
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the effect of different amounts of a substrate on the respiration rate of yeast and to compare this to the effect of different amounts of glucose on the rate of yeast respiration. The substrate which I chose to further investigate was fructose. Fructose is a fruit sugar which is one of the three‚ along with glucose and galactose‚ dietary monosaccharides that are directly absorbed into the bloodstream during digestion. Materials: 2% yeast solution Large beaker Small beaker Conical flask
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An experiment to demonstrate the effect of temperature on fermentation by yeast The purpose of this laboratory is to observe how temperature affects the metabolism of Grape juice by visibly noting the volume changes of identical food mixes containing yeast at different temperatures. Background Information If yeast is added to a liquid containing sugar and other nutrients‚ kept at an appropriate temperature (and deprived of oxygen)‚ it will turn the sugars into ethanol (alcohol) and carbon
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CHAPTER 4: ENZYMES Enzymes are biological catalysts. There are about 40‚000 different enzymes in human cells‚ each controlling a different chemical reaction. They increase the rate of reactions by a factor of between 106 to 1012 times‚ allowing the chemical reactions that make life possible to take place at normal temperatures. They were discovered in fermenting yeast in 1900 by Buchner‚ and the name enzyme means "in yeast". As well as catalysing all the metabolic reactions of cells (such as respiration
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Lab 8 Cellular Respiration and Fermentation Objectives: 1. Observe the effects of cellular respiration on temperature in a closed system. 2. Investigate carbon dioxide production in both germinating pea seeds and crickets. 3. Perform an investigative study of the rate of cellular respiration in both pea seeds and crickets at various temperatures. 4. Compare the alcoholic fermentation of glucose‚ sucrose‚ and starch by yeast. Introduction All organisms must have a continual
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