Investigation of the Peroxidase Enzyme & Temperature Abstract: In this lab we tested the effect temperature has on the rate of enzyme activity. The way we figured this out was by taking four different temperatures and testing the different absorbance levels they produced every 20 seconds for two minutes straight using a spectrophotometer. The important part of this experiment was the temperature the enzyme concentration was made at. What we got from the experiment was at lower temperature we
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amount of tenderizer did not break down as much as the cup â»3 with the most tenderizer. Conclusion According to this lab it demonstrated that Jell-O does have protein since the enzymes in the meat tenderizer were able to break down the Jell-O and prevent it from setting. This data also shows that the amount of enzymes effects the breakdown of proteins since the cup with the least amount of meat tenderizer set better than the cup with the most amount of meat tenderizerizer CUP NUMBER OBSERVATIONS
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of pH on Peroxidase Enzyme Claire Bell Biology 1130 Jazmen Myers Section 36 Abstract Peroxidase is a turnip enzyme; it is used in the oxidation of hydrogen peroxide to lower activation energy‚ speeding up the reaction. The activity of peroxidase is highly dependent on its environment and most importantly the pH level. Peroxidase has been the focus of many recent studies and is believed to possibly reduce swelling among other things. We conducted an experiment testing the effect
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_____ 3. Questions 15 points/ _____ Lactase enzyme lab OBJECTIVES: 1. Understand how biological molecules are essential to the survival of living organisms * * 2. Compare the structures and functions of the major biological molecules(carbohydrates‚ proteins‚ lipids‚ and nucleic acids) as related to the survival of organisms. * 3. Investigate and describe the structure and function of enzymes and explain their importance in biological systems
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questions on his own without being aided. Questionnaires are now widely used collecting data‚ particularly when data are to be collected from a large number of people who are scattered over a wide area. They are used both as indepen¬dent and separate method of collecting data. They are also used as an additional device to check data gathered through observation and personal interview. Definition of Questionnaire: 1. “A questionnaire is a means of gathering information by having the respondents fill
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Experiment #1 1. At substrate concentration‚ the enzyme is working at “maximum efficiency.” With a concentration at 40‚ it produced 2‚339 products. 2. The maximum velocity of a reaction is reached when the active sites are almost continuously filled. Increased substrate concentration after this point will not increase the rate. The reaction rate increases as substrate concentration is increased. It will soon level off though. 3. When the concentration is at low substrate‚ most of the enzyme
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Running head: LAB 5: INVESTIGATING AN ENZYME-CATALYZED REACTION Lab 5: Investigating an Enzyme-Catalyzed Reaction September 24‚ 2014 Principles of Biology 120.601 Mrs. Annemarie Duncan Abstract: (Burmania) This experiment was performed in order to examine ways in which a potato catalase enzyme reacts to various assays with differing variables. To do so a baseline assay (undiluted extract and room temperature H2O2) was used within the experiment with only one other variable changed in
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SCIENCE TEST #1 SEPTEMBER 2013 I. SCIENTIFIC METHOD a. The scientific method steps are: 1. Problem or question 2. Hypothesis 3. Experiment 4. Data 5. Conclusion b. The scientific method is a logical organized mechanism for identifying and researching a problem and devising a strategy for solving it. c. In the problem or question step‚ the researcher must decide what it is that you will be studying what you wish to learn.
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pH & Enzyme Action Aim: To inspect the effects of the pH on enzymes. Apparatus: 100 cm³ Beaker 3 – 5cm³ Syringes 2 Test Tube Racks with 8 Test Tubes Stop-watch Ruler Dropping bottle of detergent Marker Pen Masking Tape 400cm³ Hydrogen Peroxide 200cm³ Liver Catalase Solution 100cm³ of following Buffer Solution – pH5 pH7 pH9 pH11 Method: The materials were collected. The test tube rack one with 4 test tubes had been labelled A to D. The 2cm³ of each buffer solution
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Activity of the Enzyme Catalase in breaking down Hydrogen Peroxide and the effect of various factors on Enzyme Activity Introduction The enzyme catalase is present in cells in order to speed the breakdown of hydrogen peroxide (H2O2)‚ which is a toxic chemical to the human body. When hydrogen peroxide is broken down‚ the end products are Water (H2O) and Oxygen (O2). In this report‚ the reaction of catalase to hydrogen peroxide is being tested. Furthermore‚ the effects of temperature‚ concentration
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