the Effect of Temperature on Enzyme Activity Almost all chemical reactions that occur in living organisms are catalyzed by enzymes. Many factors in a cell’s environment affect the action of an enzyme. In this investigation‚ you will design an experiment to determine the effect of temperature on an enzyme-catalyzed reaction. You will complete an entire lab write-up using the CHS LAB REPORT FORMAT. Fundamental Question How does temperature affect the rate of an enzyme-catalyzed reaction? Materials
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Title: Enzyme Activity Lab Purpose: To measure the rate of enzyme activity from a tissue abstract and experiment with different factors‚ such as the enzyme solution and the substrate with different hydrogen peroxide percentages and temperature‚ that affect enzyme activity. Hypothesis: 1) If the disk is placed into each beaker with 100 units/ml of enzyme solution‚ then the time for the disk to float will be 30 seconds. 2) If the temperature of the solution is at 5 degrees Celsius‚
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pH on Enzyme Activity Athenaa Jeyachandran Mr.Menes Course Code January 21st‚ 2013 The Effect on Enzyme Activity Due to Temperatures Purpose: The purpose of this lab experiment is to examine how different temperatures affect enzyme activity using hydrogen peroxide. Hypothesis: My hypothesis for this experiment is that temperatures near body temperature is when enzyme activity will be at its highest. I believe this will occur because in our body‚ enzymes are very
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analogue experiment blind design case study confound control group correlation correlational method dependent variable double-blind design epidemiological study A research method in which the experimenter produces abnormal-like behavior in laboratory participants and then conducts experiments on the participants. An experiment in which participants do not know whether they are in the experimental or the control condition. A detailed account of a person’s life and
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BIO 5 Lab Report: Lactase Enzymes Enzymes are biological catalysts or assistants. Enzymes consist of various types of proteins that work to drive the chemical reaction required for a specific action or nutrient. Enzymes can either launch a reaction or speed it up. The chemicals that are transformed with the help of enzymes are called substrates. In the absence of enzymes‚ these chemicals are called reactants. Enzymes are thought to have an area with a very particular shape. When a molecule of
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After conducting the experiment and gathered the required data‚ we came to conclusion that certain environmental factors affect the enzyme activity rate. For the first experiment‚ where we tested the increase in concentration of enzyme with the substrate‚ we found that higher concentration of enzyme increases the rate of reaction of the enzyme. This is because more enzyme molecules are present‚ which allow more substrate molecules to get into the active sites of the enzyme (Sattler W& Esterbauer
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Enzymes are generally protein macromolecules that act as catalysts in metabolic reactions. A catalyst is a chemical agent that speeds up a reaction without being consumed by the reaction. Enzymes speed up metabolic reaction rates by lowering the activation energy barrier‚ which is the amount of energy initially needed to spark a reaction. It allows reactant molecules to absorb enough energy to break bonds and react without raising the temperature to an extreme. During this process the substrate
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EXPERIMENT 11: DETERMINATION OF DISSOLVED OXYGEN IN A WATER SAMPLE (WINKLER METHOD) INTRODUCTION In an alkaline solution‚ dissolved oxygen will oxidize manganese(II) to the trivalent state. 8OH-(aq) + 4Mn2+(aq) + 2H2O(l) －－＞ 4Mn(OH)3(s) The analysis is completed by titrating the iodine produced from potassium iodide by manganese(III) hydroxide. 2Mn(OH)3(s) + 2I-(aq) + 6 H+(aq) －－＞ 2Mn2+(aq) + I2(aq) + 6H2O(l) Sodium thiosulphate is used as the titrant. Success of the method is critically
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Abstract: The Enzyme Lab results where when the liver was frozen‚ its reaction was fast‚ and when it was hot‚ it was slow‚ and the liver that was at room temperature reacted slowly to medium. Introduction: The Enzyme Lab is to conduct investigations to determine the most favorable conditions for the most efficient enzyme activity. Variables to be used testing include temperature‚ pH values and surface area. Enzymes are proteins that speed up the rate of chemical reactions‚ which would otherwise
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peroxide varying in concentration 1‚2‚4‚6%‚ fresh liver(containing catalase)‚ sharp knife‚ forceps‚ cutting tile‚ 2x 10mL measuring cylinder‚ 1x 100ml measuring cylinder‚ 1x 50mL measuring cylinder‚ stop watch‚ detergent‚ teat pipette‚ distilled water. Method: 1) The materials for the practical were collected. 2) We set up a clean cutting area which consisted of placing newspaper onto the bench followed by the cutting tile on top. The liver was then cut into 1 cm cubes. 3) A 1 cm cube of liver was placed
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