Environmental Science The Scientific Method Amber Steadham October 31‚ 2012 Abstract: We conducted several experiments using the Scientific Method. We made observations‚ recorded them and used our observations to propose a hypothesis. The experiments included chemical and physical reactions dealing with torn news paper‚ appearance of rock salt crystals‚ the appearance of sand‚ oil and water on plastic‚ and making iodine in water and mineral oil. We found that all these included four physical
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for the effects of temperature on the enzyme activity was that the reaction’s rate would increase as the temperature increased‚ until they go over the optimum temperature where the enzymes denature and the reaction’s rate quickly drops to zero. At 5 degree C the rate is 0.00059mole PNP/min. This then increases to 0.01031mmoles PNP/min at a temperature of 50 degree C. The rate then drops drastically to -0.00215moles PNP/min. This point is where the enzymes have been denatured and have no activity
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two Title: Enzyme Function Purpose: To observe the role of enzymes in chemical reactions and to determine the kinds of cells that contain more of the enzyme catalase. Prior Knowledge: Enzymes are proteins that assist the chemical reactions of a cell by lowering the amount of activation energy needed to start the reactions‚ thereby enabling the cell to carry out the reactions at a faster rate; enzymes that lower the activation energy are therefore called catalysts. Moreover‚ the enzyme itself is
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Introduction Enzymes are key players in metabolism. A metabolism is the organic processes in a cell or an organism that are necessary for life. An enzyme affects the rate at which a reaction occurs when the activation energy is lowered. In this reaction the reactant is called the substrate which is that combine with enzymes molecules to form a temporary enzyme substrate complex. During this products are formed and the enzyme molecules released is unchanged. For the substrate complex to form the
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from sunlight. Plants‚ algae‚ and some bacteria use the energy captured during photosynthesis for their metabolic reactions. During photosynthesis in plants‚ chlorophyll and enzymes in leaves convert certain wavelengths of light into chemical energy. A simple equation can be used to represent photosynthesis. Enzymes and Chlorophyll light energy CO2 H2O carbohydrate O2 In this investigation you will examine the relationship between the amount of light energy available and the rate of use
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Effect of temperature of the reaction: The effect of the temperature of the reaction on the activity of the purified enzyme was carried out by make the enzymatic reaction for 10 minutes at different temperature 25‚30‚35‚40‚45‚50‚60 and 70°C using an enzyme protein 0.1mg/reaction mixture and substrate concentration of 15 mg/reaction mixture‚ using a control of previously heated enzyme solution in the reaction. The data recorded in (table 27) and (figure 29) illustrate the effect of temperature of the
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Throughout the experiments the size of the balloons stayed about the same size except for in experiment 3. Also the bottles that had more sugar had a stronger scent of alcohol and also contained more foam on top of the liquid. All yeast in each of the bottles seemed to have fermented at the same time and stopped/slowed down‚ the growing process‚ at the same time. Bottle 3 also would always overflow and the foam would fill the ballon in every experiment. Another trend with bottle 3 was that in the
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Experiment 4 – Effect of Temperature on Enzyme Activity Aim To study the effects of temperature on the activity of amylase enzyme on starch solution. Introduction Enzymes are widely known as biological catalyst. Almost all cellular reactions are controlled and guarded by enzymes. Virtually every metabolic reaction which takes place within a living organisms are catalyzed by enzymes. Enzymes are complex three-dimensional globular proteins. Some of the enzymes are built up off proteins and
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Banana and Papaya Fruit Peelings Extract as an Alternative Culture Medium for Fungi Submitted by: Maricel P. Clores Catherine A. Bernardo Rodora Lafiguera Julius Jorge Suarez Scope and Delimitation This study is focused on how to produce ointment out of banana and papaya fruit peelings extract to help avoiding fungal infections. It must be done within a laboratory. The place must be conducive for only a matter of years. This study builds upon on how to lessen fungal
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Received July 18‚ 1997X A radiochemical enzyme assay for studying cyclooxygenase (COX)-catalyzed prostaglandin biosynthesis in vitro was optimized with respect to both COX-1 and COX-2 activity. The assay can be used to assess the relative selectivity of plant-derived inhibitors on COX-1 and COX-2. Assay conditions were optimized for both enzymes with respect to concentration of cofactors (l-epinephrine‚ reduced glutathione‚ and hematin)‚ activation time (enzyme and cofactors)‚ reaction time‚ and
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