I. Title. Restriction Enzyme Mapping of pBR322 Using Agarose Gel Electrophoresis. II. Authors. Author: Partner: Section: Thursday‚ 1:10 pm Date of Experiment: October 25‚ 2012 III. Introduction. Restriction enzymes (or restriction endonucleases)‚ originally isolated from Haemophilus influenzae in 1970‚ are enzymes within a cell that cleave foreign DNA within a specific and predictable nucleotide sequence (known as a restriction site) regardless of the source of such DNA. Such restriction
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Enzyme Kinetics Examples and Problems 1. An enzyme is produced for producing a sun protection lotion. Given kinetic data for the enzyme reaction with Vm=2.5 mmol/m3.s‚ Km=8.9 mM and So=12mM‚ what would be the time required for 95% conversion in a batch reactor? 2. An enzyme was assayed at an initial substrate concentration of 10-5M. The Km’ for the substrate is 2x10-3M. At the end of 1 min‚ 2% of the substrate had been converted to product. a. What % of the substrate will be converted
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Helicopter Experiment Prediction: As the mass on the helicopter’s copters increases‚ the speed taken for the rotors to spin down faster‚ this is because there will be more downward pull from gravity‚ as the terminal velocity for a paper helicopter is low. Variables: Independent variable will be the mass that we change throughout the experiment. The dependant variable will be the time that we measure for the helicopter to reach the floor. Finally the controlled variables include‚ the helicopter
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Lux G experiment is performed at the University of California San Diego on May 21st until June 4th of 2018 by Gloria Karem. This experiment is based on the purification of LuxG by using multiple purification and lysing methods: Brad Ford Assay‚ SDS-PAGE‚ affinity purification‚ lysozyme treatment‚ nickel column‚ sonication‚ and flavin reeducate activity assay. His-tagged protein is expressed in E. coli from pGhis‚ with the T7 RNA lac repressor induction system.5 Sonication is used‚ it’s why bacteria
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The effect of alcohol concentration on the cell membrane Abstract: In this experiment I found out that as the concentration of the alcohol was increased so did the colour intensity of the solution. This is because the higher concentration of ethanol‚ results in more damage done to the cell membrane‚ resulting in leakage of red pigment from the cell. If the membrane is damaged more‚ more red pigment will leak out of the membrane and into the ethanol. Hypothesis: The higher the concentration
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- Quasi Experimental Design: If the researcher lacks control over the assignment of participants to conditions and does not manipulate the causal variable of interest‚ the design is quasi experimental. o Quasi Experiments do not have internal validity because participants are not randomly assigned to conditions and the researcher may have no control over the independent variable. - Basic Confounds in Quasi Experimental Design are : o History o Maturation (Emotional‚ Physical & Psychological)
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Title: An investigation into the impact of group pressure on an individual’s estimate of the amount of beads in a pot (ginger granules in a jar). IV = Group/Individual DV = Individual beads estimate Abstract This experiment investigated the impact of group pressure on the individual. The hypothesis is that group pressure does indeed impact on the individual and in this case the individual’s estimate of the number of ginger granules in a jar. Participants were asked to make a judgement of
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Cebu Institute of Technology – University Cebu City PHYSICS DEPARTMENT Physics 201 – G01/M02 Name/Course/Year: Noel S. Alvarez BSIT – II Date Submitted: November 29‚ 2012 Experiment No. 1 Group No./Time/Day: 3 / 12NN-3PM/Thurs MEASUREMENT‚ ERRORS AND DENSITY I. Objective: a. To know the principles of good measurement and to compare the precision and accuracy of some commonly used measuring devices. b. To study the types‚ causes and effects of errors in measurement
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increases. Equipment: * Culture of water flea – Daphnia * Caffeine of 5 different concentrations * Microscope * Counter * Cavity Slide * Pipettes * Stop clock * Distilled water * Test tubes * Petri dish Method: 1. Remove one Daphnia and place it in the cavity slide 2. Remove the pond water and replace it with distilled water 3. Leave it for 5 minutes to acclimatize‚ then observe the heart rate under the microscope for 30 seconds‚ multiply the
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Abstract The aim of the study is to discover the effect caffeine has one ’s short term memory. The method used was a mixed design‚ a combination of the repeated measures and independent measures designs. The participants used were chosen from a sample opportunity‚ and all were twelfth grade Biology students. The results indicate that the lowest average score was that of the experimental group‚ after
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