Biology class split into groups "to investigate inhibition of enzyme activity by specific chemicals called inhibitors" (1). Group three pondered this lab ’s inhibitor‚ phenylthiourea (PTU). Is it a competitive inhibitor? That is an inhibitor that literally "competes" with the substrate by mimicking it‚ and thus "wins" the position at the active site of the enzyme. The blocked substrate in turn cannot join with the active site‚ causing the enzyme ’s activity to be hindered. This inhibition can be reversed
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Determination of the activation energy of an enzyme catalysed reaction Introduction In this practical the aim for this experiment was to find out the catalytic power of alkaline phosphate‚ as well as the rate of reaction and the activation energy of p-nitrophenol phosphate. Enzymes are biological molecules that catalyse a chemical reaction. ‘Enzymes work by lowering the activation energy of a chemical reaction making it easier to proceed’ [1]. This allows molecules to have more energy therefore
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Enzyme assay lab report Health and safety: 4-nitrophenol is harmful. Introduction: Enzymes are quaternary structured proteins that are specific biological catalysts that speed up a reaction without being used up. They contain an active site that allows substrate to bind to a specific area on the enzyme which is of a complimentary shape of the substrate. There are two models of enzyme action‚ the Lock and Key model and the Induced Fit model. The Lock and Key model states that the enzyme has a specific
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Enzymes are responsible for many crucial processes in living cells since they accelerate chemical reactions which would occur too slowly‚ or would lead to different products without their contribution. Enzymes are biocatalysts that usually show high affinity to a specific substrate under particular environmental conditions. The binding of the substrate and catalysis take place at a specific small region‚ around 10 amino acids‚ in the enzyme known as active site which usually represents a hydrophobic
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Principles of Biology Lab Exercise Enzymes: Catalysts of Life Instructor: Professor Alcendor By Shahid Rana Date: March 7th‚ 2013 Abstract: In this experiment we have demonstrated the function of enzymes. The whole experiment was devoted to understand how enzymes work as a catalysts and increase the chemical reaction without being used themselves. In general‚ enzymes are proteins that function as biological catalysts. These enzymes adhere to lower to amount of energy required for
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SBI 4U0: Enzyme Lab Purpose: To compare the action of the enzyme catalase‚ to a non-protein catalyst under different conditions. Observations: | | |Observations |Rate of Reaction |Interpretations | |A |Sand |- Sand piled up at the bottom of |0 |- There is no reaction between sand and| | |
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the beginning of the lab. Post-lab questions for Topic 5 – Enzymes Name: Date: Group: T W R Formation and Detection of Benzoquinone Table 1. Formation and Detection of Benzoquinone: Record Absorbance Time 2A-Potato extract + cathecol 2B- Potato extract + water 2C- Catechol + water After 10 min 1- What were the substrate‚ enzyme and product of the enzymatic reaction? 2- What is the purpose of tubes 2B and 2C? Enzyme Specificity Table 2 – Specificity of Cathecol oxidase for different
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Biology Exam Review Unit One – Biochemistry What is an isotope? Isotope - An isotope is all atoms of the same element that have the same number of protons‚ but they may have different numbers of neutrons in the nucleus. - This means that all atoms with the same atomic number can have different atomic masses. - Because they have the same number of protons and electrons‚ they behave exactly the same in chemical reactions. Radioisotope - The nuclei of some isotopes of an element are unstable
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All living organism depend on catalytic proteins called enzymes‚ these compound are responsible for metabolism and biochemical regulation of energy‚ there are millions of enzymes and hundreds of enzymes have been identified in the human body as well. All living system possess enzymes as vital components of the body‚ enzymes are found in all organisms. Everyone has a natural pH balance in the body. Enzymes are complex proteins which speed up chemical reactions if this did not happen it would occur
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The purpose of this review paper is to discuss the effect of temperature on enzyme- catalyzed reactions. This is relevant because many diseases can be diagnosed and controlled by the processes of enzyme activity (Worthington 2015). If more information is not found about enzyme activity and how it is affected‚ many diseases may go undiagnosed and uncontrolled. Temperature is a type measurement that does not only consist of heat. It is the measurement of the mean kinetic energy of any group of particles
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