Research Question: How will an increase of FeSO4 concentration affect the activity of the enzyme catalase? Hypothesis: By increasing the concentration of FeSO4‚ there will be a decrease in the activity of the enzyme catalase because the Iron (ii) in the metallic ion will act as an inhibitor during the catalysis of the hydrogen peroxide into O2 and H2O. Introduction: We rely on oxygen to provide us with energy (ATP) and go through all the phases of aerobic respiration. However‚ oxygen can be
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and Irish Potato Hypothesis: Polyphenol Oxidase enzyme activity can be detected by change in colour of solution‚ Inhibitors prevent the reaction of the enzymes with substrates‚ the enzyme is relatively specific. Aim: To design and conduct an experiment to demonstrate enzyme activity of Polyphenol Oxidase and Peroxidase when mixed with catechol‚ caffeic acid‚ pyrogallad‚ tyrosine‚ guacol‚ and water‚ to test the effect of inhibitors on these enzymes‚ to show the specificity of Polyphenol Oxidase and
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4 Enzymes and metabolism 4.1 Metabolism (Book 1A‚ p. 4-3) Metabolism (新陳代謝) refers to the (1) _______________ of the chemical reactions that take place in an organism to keep it alive. Catabolism (分解代謝) Anabolism (合成代謝) All the (2) _______________ reactions within an organism (3) _______________ energy All the (4) _______________ reactions within an organism (5) _______________ energy 4.2 Properties and actions of enzymes (Book 1A‚ p. 4-4) A What is the role of enzymes in metabolism?
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Enzyme Study Questions 1. Definitions/terminology: o enzyme: a protein molecule that catalyzes chemical reactions without itself being destroyed or altered o catalyst: a substance that increases the rate of a chemical reaction but is not consumed or changed by it. o substrate: a substance upon which the enzyme acts. o denaturation: the partial or total alteration of the structure of a protein without change in covalent structure by the action of certain
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Unit 2: Cell Biolo SCIE206-1401B-08 Photosynthesis and cellular respiration make one large cycle that sustains life on earth. Through photosynthesis the suns energy is constantly being transformed into glucose‚ which is a chemical energy. Respiration is the chemical activity that occurs in all plants and animal cells that release the energy from the glucose. Photosynthesis cannot exist with cellular respiration‚ they are completely linked together in providing the energy through
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precipitate. Enzymes Enzymes are protein molecules that act as biological catalysts. An enzyme catalyses a specific reaction. They have an active site… Substrate ENZYME Products. Maltose is broken down by the enzyme maltase to glucose and glucose. The reactions enzymes catalyse can be anabolic or catabolic - Anabolic meaning building‚ catabolic meaning breaking down. They do not die. They are simply denatured. They are affected by temperature‚ pH and concentration of enzyme and/or substrate
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Energy and Metabolism Chapter 6 Energy and Metabolism • Organisms require the constant input of free energy and matter for growth‚ reproduction‚ and maintenance of living systems • Life requires a highly ordered system ▫ What is free energy? Energy available to do work in any system G What other types of energies do cells use? • Potential ▫ Stored energy ▫ Chemical • Kinetic ▫ Energy of motion Thermal Energy • • • • All forms of energy can be converted to heat Thermodynamics
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Experiment 10 – Enzymes Enzymes are proteins that act as catalysts for biological reactions. Enzymes‚ like all catalysts‚ speed up reactions without being used up themselves. They do this by lowering the activation energy of a reaction. All biochemical reactions are catalyzed by enzymes. Since enzymes are proteins‚ they can be denatured in a variety of ways‚ so they are most active under mild conditions. Most enzymes have optimum activity at a neutral pH and at body temperature. Enzymes are also
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This experiment investigated the kinetics of the enzyme glycogen phosphorylase b which is important to metabolism. AMP is an allosteric activator of the enzyme because it converts glycogen phosphorylase b from its T state to the R state which is the active form. Caffeine is an inhibitor because it binds the nucleoside inhibitor site. When it binds this site‚ it stabilizes the inactive T state and blocks the catalytic site which needs to be open for enzyme activity to occur. The glycogen phosphorylase
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determine the effect of various factors on the rate of reaction between an enzyme andits substrate‚ and also to determine the optimal ranges under which the enzyme activity ismaximized. Also to determine whether saline and alcohol are inhibitors or activators Hypothesis: PH factor prediction: I predict that as the pH increases so the activity of the enzyme willincrease until it reaches optimum pH range (pH 7) because the enzyme is less denaturedwhen it reaches the preferred pH level‚ and after this
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