Temperature Affecting Enzyme Activity Introduction The basic properties of life revolve around chemical reactions. Without the presence of enzymes some of life’s processes would not come so easily. Enzymes are basically proteins‚ which have specific shapes for different substrates. Enzymes change the rate in chemical reactions. It does this without having to change its own shape‚ which makes enzymes different from other proteins. A common enzyme that we have is catalase‚ which breaks down
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Erin Arroyo Lab report June 11‚ 2013 Biology 123 Professor K Title: Scientific Investigation of the Peroxidase Enzyme & Temperature Abstract: In this lab we tested the effect temperature has on the rate of enzyme activity. The way we figured this out was by taking four different temperatures and testing the different absorbance levels they produced every 20 seconds for two minutes straight using a spectrophotometer. The important part of this experiment was the temperature
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pH‚ and concentration of substrate‚ on the activity of the enzymes. By conducting these three separate experiments also‚ three graphs are able to be obtained where the trend of each factor affecting on the enzyme activity is shown and described clearly. II. Hypothesis Experiment 1 (Effect of Temperature): As the temperature increases‚ the height of the bubble will increase too‚ indicating a faster rate of reaction. Experiment 2 (Effect of pH): Enzymes are affected by changes in pH. Extremely
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Coefficient of Kinetic Friction Objective: After completing this experiment‚ you should be able to measure the coefficient of sliding friction of an object that is located on an inclined plane. Apparatus: Inclined Plane Pulley attachment for plane Wooden blcok‚ with hook attached Meterstick Felt Set of masses String Protractor Procedure: 1. Place the board in the position shown in Figure 7-1. 2. The angle of the board should be small enough so that the wooden block will not slide
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correlation between catalase concentration and the rate of reaction. This occurs because as the enzyme concentration increases‚ there are more enzymes available to catalyze substrates. More enzymes means more reactions can take place at a time‚ thus a faster rate of reaction. Overall‚ based on the results of table 2.0 and graph 2.0‚ it is prevalent that there is a positive correlation between the concentration of enzymes and the rate of
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Kinetic energy is increasing between letters A-B‚ C-D and E-F. Based off the observations‚ in phase A-B Tert-Butyl Alcohol stays a solid‚ in phase C-D Tert-Butyl Alcohol stays a liquid‚ and in phase E-F the chemical stays a gas. This is observed at the particle level because temperature is a measure of Ek; the temperature is increasing which increases kinetic energy. Since Ek is the energy of motion‚ the particles would increase their velocity and the number and force of collisions. However‚ the
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Abstract The “Chemical Kinetics” experiment was done to investigate the changes in the rate of reaction under the effect of concentration‚ temperature‚ and presence of a catalyst. It was determined that as the concentration of reactants and the temperature increases‚ the rate of the reaction increases as well. Also‚ the reaction was run by the presence of catalyst‚ and the rate of the reaction increased drastically in the presence of it. The order of the reaction with respect to each reactant
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The Effects of Varying pH on Enzyme Activity A lab was conducted to test different pH balances on an enzyme. Introduction: Enzymes are protein catalysts that speed up a chemical reaction without being consumed in the process. Enzymes are three-dimensional structures that consist of one or more polypeptide chains. The polypeptide chains form an active site (where a substrate will fit into). Enzyme molecules are folded into a very specific shape held together by the different forces of attraction
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for evaluation of kinetic constants (Km and Vmax) of extracellular purified pectinase. 3.8.3. Effect of temperature of the reaction: The effect of the temperature of the reaction on the activity of the purified enzyme was carried out by make the enzymatic reaction for 10 minutes at different temperature 25‚30‚35‚40‚45‚50‚60 and 70°C using an enzyme protein 0.1mg/reaction mixture and substrate concentration of 15 mg/reaction mixture‚ using a control of previously heated enzyme solution in the
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Effect of Temperature on Enzymes ~Abstract~ In this experiment the effect of different types of temperatures on enzyme activity was examined. The temperature baths that were used to test the difference in enzyme activity on fresh liver were; 4 °C‚ room temperature which was 22°C‚ body temperature which is 37°C‚ and 77°C. The total time of each trial was 2 and a half minute‚ 1 minute for the H2O2 to acclimatize to the temperature‚ 1 and a half minutes for the reaction to occur. Catalase causes Hydrogen
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