Factors Affecting Enzyme Activity Abstract: In the following lab factors affecting enzyme activity‚ temperature‚ pH‚ enzyme concentration‚ substrate concentration and surface area will be tested on a beef liver enzyme to see if there will be any effect of performance. By doing 2 or more trials the results will show whether there is an effect to the enzyme from the following factors or not. Some of the factors may denature the enzyme and some will do nothing. Using a table qualitative and the
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LABORATORY REPORT (Click on the Save a Copy button on the panel above to save your report) Activity: Enzyme Activity Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 60 °C (140 °F) 3. Sucrase activity decreases with increasing sucrose concentration. Materials and Methods Effect of pH on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and fructose) produced 2. Independent Variable. pH 3. Controlled Variables
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Lecture 3: Enzyme kinetics Tue 17 Jan 2006 with the collaboration of Luna De Ferrari 1 Images from: D. L. Nelson‚ Lehninger Principles of Biochemistry‚ IV Edition‚ W. H. Freeman ed. A. Cornish-Bowden Fundamentals of Enzyme Kinetics‚ Portland Press‚ 2004 A. Cornish-Bowden Enzyme Kinetics‚ IRL Press‚ 1988 Computational Systems Biology Summary: • • • • • • 2 Simple enzyme kinetics Steady-state rate equations Reactions of two substrates Inhibition of enzyme activity pH dependence
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Title: Enzyme Activity Lab Purpose: To measure the rate of enzyme activity from a tissue abstract and experiment with different factors‚ such as the enzyme solution and the substrate with different hydrogen peroxide percentages and temperature‚ that affect enzyme activity. Hypothesis: 1) If the disk is placed into each beaker with 100 units/ml of enzyme solution‚ then the time for the disk to float will be 30 seconds. 2) If the temperature of the solution is at 5 degrees Celsius‚
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In this lab we tested the effect of temperature has on the rate of enzyme activity. The way we figured this out was by taking four different temperatures and testing the difference absorbance levels they produced every 20 seconds for about 2 minutes straight using a spectrophotometer. The important part of this experiment was the temperature the enzyme concentration was made at. What we got from the experiment was at lower temperature we got very low numbers for the absorbance‚ which gave us a lower
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LAB: Substrate Concentration Affecting the Rate of Enzyme Activity: Through the Experiment of Beef Liver Puree and Hydrogen Peroxide Research Question Does different amount of substrate affect the rate of enzyme activities? Purpose To examine how different types of concentration (Hydrogen Peroxide) affect the rate of enzyme activity. Hypothesis We believe that if there is more substrate concentrated‚ then there will be an increase in the rate of enzyme activity. This is because
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temperature on the enzyme activity was that the reaction’s rate would increase as the temperature increased‚ until they go over the optimum temperature where the enzymes denature and the reaction’s rate quickly drops to zero. At 5 degree C the rate is 0.00059mole PNP/min. This then increases to 0.01031mmoles PNP/min at a temperature of 50 degree C. The rate then drops drastically to -0.00215moles PNP/min. This point is where the enzymes have been denatured and have no activity‚ shown as the last
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Temperature and Enzyme Activity Aim: To investigate the effect of temperature on enzyme activity Hypothesis: As the temperature deviates from 40°C the activity will lower Equipment: – Chemicals: – Milk – Junket tablets – Hot water – Ice – Test tubes – Stopwatch – Measuring cylinder Risk Assessment: |Hazard |Risk |Prevention | |Hot Water
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INTRODUCTION Enzymes are a protein serving as a catalyst‚ a chemical agent that changes the rate of the reaction without being consumed by the reaction. Enzymes are proteins made up of long chains of amino acids. These form complex shapes. The enzymes are individuals‚ like the different players on a ball team‚ they have different specific structures and jobs. As one ball player may be very tall and one short‚ the specific different shape of the active site on an enzyme is unique and prepares it
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My lab group studied the effect of PH on reaction rate/ enzyme activity measured by foam height. PH is the measure of the concentration of hydrogen ions in a solution. The higher the hydrogen ion concentration‚ the lower the pH. Every enzyme has an optimal PH‚ meaning they have a very small window in which they are most active. Our enzyme (potato smoothie) had an optimal PH of 7.0-7.5. We know this because we measured the enzyme’s reaction rate by measuring foam height. The largest foam height we
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