"Enzyme kinetics tyrosinase activity lab" Essays and Research Papers

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    The activity of an Enzyme is affected by its environmental conditions and changing these factors can alter the overall rate of the reaction. Reaction rates are influenced by external factors such as pH‚ temperature and salt concentration. Different enzymes have different optimum temperatures in which they are most efficient and different pH levels which is ideal for their formation of enzyme-substrate complexes. As the temperature increases‚ there is more movement of molecules and more random collisions

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    Abstract: This lab tests how temperature and pH affect how enzymes will function. The lab showed that temperature will denature an enzyme when past its optimal working temperature and won’t denature in cold temperatures‚ but have slowed molecular activity. pH will also have an affect on an enzymes efficiency‚ when out of optimal pH the enzyme will not function as it is supposed to and if to far out of the optimal pH the enzyme will change shape and no longer work. Enzymes also showed to be reusable

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    Factors Effecting Enzymes

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    Factors Affecting Enzymes The activity of an Enzyme is affected by its environmental conditions. Changing these alter the rate of reaction caused by the enzyme. In nature‚ organisms adjust the conditions of their enzymes to produce an Optimum rate of reaction‚ where necessary‚ or they may have enzymes which adapted to function well in extreme conditions where they live.Increasing temperature increases the Kinetic Energy. In a fluid‚ this means that there are more random collisions between molecules

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    Mass and Kinetic Energy

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    puck of mass m initially at speed u collides head-on (without rotation) with a stationary puck of mass M. Find the velocities of both puck after the collision if: i) the collision is fully elastic ii) the collision if fully inelastic. i) momentum: kinetic energy: mu = mv+MV (+ve in direction of initial u) 1 /2 m u2 = 1/2 m v2 + 1/2 M V2 2 eqns in 2 unknowns: V = (u - v) m/M substitute in K eqn: u2 = v2 + (M/m) V2 = v2 + (M/m) (u - v)2 (m/M)2 = v2 + (u - v)2 (m/M) let ρ = (m/M) ⇒ v2 (1 + ρ) - 2ρ

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    Enzyme Summary

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    There are many reasons why enzymes have such a high specificity. The first variable is an enzyme’s primary structure. A primary structure is just a combination of amino acids. There are twenty different amino acids that the primary structure can be created from. Every enzyme has a different order that the acids are placed in and each one has a different number or amino acids. The slightest change in this structure can affect a protein’s conformation and function. The secondary structure is a regular

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    Enzyme Report

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    Enzyme Report Case 1 - Hereditary Fructose Intolerance 1 & 2. Enzymes take on a variety of roles in the human body at the cellular level. Specifically‚ they aid in the breakdown of macronutrients such as glucose and fructose so that the body can use them. Although reactions within the body would occur without enzymesenzymes provide control because the reaction is not needed all of the time. Enzymes function in specific environments such as temperature or pH; some enzymes are specific

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    In this essay‚ it will be discussed how the Kinetic Molecular Theory explains many gas law specifically Boyle’s gas law‚ Charles’ gas law‚ and Avogadro’s gas law. To understand what will be talked about is to first know what the Kinetic Molecular Theory is. In our textbook is says it is “A simple model for gases that predicts the behavior of most gases under many conditions.” First off‚ we will take a look at Boyle’s Law. As stated in our lab manual “at constant temperature the pressure which a given

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    Effects of Temperature‚ pH‚ Enzyme Concentration‚ and Substrate Concentration on Enzymatic Activity INTRODUCTION Enzymes‚ proteins that act as catalysts‚ are the most important type of protein[1]. Catalysts speed up chemical reactions and can go without being used up or changed [3] Without enzymes‚ the biochemical reactions that take place will react too slowly to keep up with the metabolic needs and the life functions of organisms. Catecholase is a reaction between oxygen and catechol

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    Rennin Enzyme Experiment

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    Investigating the effects of changing temperature on the activity of enzymes Background information: Renin is an enzyme that catalyses the coagulation of milk. It is found in the stomach of many animals and is used in making cheeses and junkets. It is found in the gastric juices or gastric mucosa of many mammals‚ including humans. In the human stomach‚ particularly those of infants‚ rennin works to curdle milk so that pepsin‚ another stomach enzyme‚ can further breakdown the proteins into absorbable

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    Introduction to Enzyme

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    Enzymes Lecture outlines •Catalysis profile •Activation energy & its •Enzyme & substrate substrates •How enzymes bind to •Lock & Key model •Induced-fit model •Enzyme assay Lecture outcomes • At the end of this lecture‚ students are able to: • Define the catalyst • Understand how enzymes work as catalysts‚ the concept of activation energy and enzymes-substrate binding • Explain different theories of the relation between enzymes and substrates Catalysis • It is probably

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