Title: Catalase enzyme detection Objective: To understand the function of catalase in cells that produce the enzyme‚ interpret the results of a catalase test and know their value in differentiating bacteria. Materials: 1 clean microscopic slide‚ 3% H2O2 solution‚ swabs. Micrococcus luteus‚ Enterococcus faecalis‚ patient G Procedure: 1) Scrape some cells off from each bateria to the slant and place them on glass slide. 2) Place one or two drops of H2O2. Watch for bubbling as an indication of
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Cells are the structural and functional units of all living organisms; each specialised to perform dedicated duties throughout their life span. All living organisms can be sorted into one of two groups depending on the fundamental structure of their cells. These two groups are the prokaryotes and the eukaryotes. A Prokaryote cell is the first form of cells for many millions of years until the eukaryote cell evolved from the prokaryote cell‚ which created life. Prokaryote cells are organisms made
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1. A) I would expect the active site of nylonase to consist mainly of polar amino acids with a few nonpolar amino acids as well because the substrate for nylonase is polar overall‚ but has many nonpolar bonds. What makes me think that the nylonase enzyme is polar is that the substrate that would bind to the active site of nylonase has extreme polarity between carbon and oxygen‚ and between hydrogen and nitrogen due to their differences in electronegativity’s‚ but it still has the nonpolar bonds between
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This experiment is to determine the unknown DNA plasmid using restriction enzymes and conducting electrophoresis finally comparing the resulting fragments with the known restriction map. In this lab‚ it succeeds in showing the fragments. In this report we will discuss the‚ results‚ limitations and possible errors. Introduction In biology restriction enzymes are used in several ways to modify and manipulate DNA molecules. One common use is to compare pieces of DNA from one that is unknown‚ with fragments
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| Lysozyme activity | Observed in tears and saliva | | Connie Jamieson | 3/1/2012 | | Abstract Lysozyme is an enzyme found in both tears and saliva. Each time we blink our eyes‚ our eyelids wash tears over the surface of our eyes. In addition‚ our mouth is continually producing saliva. A spectrophotometer set to 450nm was used to measure the effect of lysozyme on bacteria. The absorbance or optical density was measured for each sample at specific times with a total measuring time of
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Another postulate of the kinetic molecular theory is that gas particles are always in motion‚ like the other states of matter. But they are different in that they undergo random translational movement. In solids‚ the particles mainly experience vibrational motion and in liquids they mainly vibrate and rotate‚ with some translational motion. Gas particles move rapidly in straight lines‚ unless acted upon by another particle or the walls of a container. This continuous contact with the container leads
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Chemistry 12: Reaction Kinetics Review 1. Looking at the expressions for reaction rate‚ write expressions with which you could express rates for the following reactions. (Hint: look at what happens to reactants and products.) Recall that solid or liquids can lose or gain mass‚ gases can lose or gain volume and aqueous solutions can increase or decrease in concentration. ("a" is done as an example.) a) Mg(s) + 2HCl(aq) H2(g) + MgCl2(aq) reaction rate = mass of Mg consumed unit time b) c) or
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Running head: LAB 5: INVESTIGATING AN ENZYME-CATALYZED REACTION Lab 5: Investigating an Enzyme-Catalyzed Reaction September 24‚ 2014 Principles of Biology 120.601 Mrs. Annemarie Duncan Abstract: (Burmania) This experiment was performed in order to examine ways in which a potato catalase enzyme reacts to various assays with differing variables. To do so a baseline assay (undiluted extract and room temperature H2O2) was used within the experiment with only one other variable changed in
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EXPERIMENT 3-CHEMICAL KINETICS: THE IODINE-CLOCK REACTION J.CHAN1 and C.CABANLIG2 1NATIONAL INSTITUTE OF MOLECULAR BIOLOGY AND BIOTECHNOLOGY‚ COLLEGE OF SCIENCE 2NATIONAL INSTITUTE OF MOLECULAR BIOLOGY AND BIOTECHNOLOGY‚ COLLEGE OF SCIENCE UNIVERSITY OF THE PHILIPPINES‚ DILIMAN‚ QUEZON CITY 1101‚ PHILIPPINES DATE SUBMITTED: JANUARY 8‚ 2013 DATE PERFORMED: DECEMBER 5‚ 2012 ------------------------------------------------- ABSTRACT The kinetics of the reaction between persulfate (S2O82-)
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Down the Hill Lab Report Kinetic & Potential Energy Name: Corinne Chen Block: 1 – 3 Date: May 22nd‚ 2013 Purpose: To investigate and compare the potential energy‚ kinetic energy and speed of a cart on a hill 1 kg h 1 m Materials: 1. 2. Cart & 1 kg mass 3. Board 4. Timer
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