Enzymes are proteins or nucleic acids that catalyze reactions. They are able to speed up reactions by reducing the activation energy of a reaction. Each kind of enzyme has a specific shape that matches its substrate so it can bind to its active site. Enzymes convert their substrates into a product. Enzyme activity are affected by factors such as temperature‚ pH‚ and time. If an enzyme is exposed to extreme heat‚ it will become denatured‚ that is‚ to become deformed and lose its original shape which
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Pablo Cuate Biochemistry Lab Report Objective: The purpose of this lab is to analyze enzyme activity at pH neutral for acid phosphatase as the colorless p-nitrophenylphosphate (PNPP) substrate concentration changes. We will empirically determine the concentration of production formation‚ Vmax and Km from absorbance of yellow PNP in the reaction. Results Figure 1 This figure indicates a positive linear trend for the concentration of PNP and absorbance. Table 1: Absorbance Value for Acid Phosphatase
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Chemical Kinetic Models Simplified Global Chemical Kinetic Model In order to compare the two reactors used in our experiment‚ a simplified global kinetic model was used to describe the DRM reaction behavior in different plasma reactor. This model was already used by authors in the field of pollutant removal by using a plasma reactor [15‚ 21–23]. It is generally accepted that free radical processes are the main mechanisms in non-equilibrium plasma reaction [21‚ 24-25]. The chemical kinetics model
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CHEMICAL KINETICS RESULTS AND DISCUSSION The balanced equation of the reaction between sodium thiosulfate and Hydrochloric acid is: S2O32- (aq) + 2 H+ (aq) SO2 (g) + S (s) +H2O (l) Using beakers with the same diameter was very important in conducting the experiment. If different sizes were used‚ the visibility of the “x” on the paper beneath the beaker could disappear from view too early or too late than the hypothetical time depending on the depth of the solution. It was also significant
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Enzyme Catalysis Introduction: Enzymes are produced by living organisms as proteins. These enzymes perform as catalysts to bring about a chemical reaction. In fact‚ most reactions are catalyzed by enzymes during reactions in the cell or in the human body. A catalyst that enzymes pose ad are by definition substances that are capable of initiating or speeding up a chemical reaction. Catalyst are not a necessity during a chemical reaction‚ they are just used to speed up a chemical reaction. This event
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review paper is to discuss the effect of temperature on enzyme- catalyzed reactions. This is relevant because many diseases can be diagnosed and controlled by the processes of enzyme activity (Worthington 2015). If more information is not found about enzyme activity and how it is affected‚ many diseases may go undiagnosed and uncontrolled. Temperature is a type measurement that does not only consist of heat. It is the measurement of the mean kinetic energy of any group of particles (Science Online 2008)
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Lambda DNA with restriction enzymes. Aim: The objectives of this experiment are: Become more familiar with using micropipettes. Use restriction enzymes to cut DNA at specific sites. Use Ligase to rejoin some of the cut/separated DNA fragments. Learn to separate DNA using electrophoresis. Introduction: Restriction enzymes are proteins which cut dsDNA at specific regions depending on the enzyme used‚ determined by the nucleotide sequence of the DNA‚ i.e. each enzyme recognises specific nucleic
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The objectives of this experiment were to investigate the activity of enzymes‚ components that influence the enzyme’s activity‚ identify an unknown phosphatase‚ influence of inhibitors‚ and determine if inhibition is competitive or noncompetitive. A spectrophotometer evaluated the measurement of color change over a period time due to product being formed. Determining unknown phosphatase and effects from different inhibitors were determined by varying the pH and substrate concentrations. The unknown
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Kinetics of a Reaction I. List of reagents & products 1. 1.0 M Copper(II) nitrate (Cu(NO3)2‚ 0.10 M Hydrochloric Acid (HCl)‚ 0.010 M Potassium Iodide (KI)‚ 0.040 M Potassium Bromate (KBrO3)‚ 0.0010 M Sodium Thiosulfate (N2S2O3)‚ 2% Starch solution‚ Water (H2O) II. Summary of Procedure. Part 1: Find the Volume of One Drop of Solution 2. Fill pipet with 3ml of distilled water 3. Mass a beaker and record 4. Put 5 drops of water into beaker and record
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Enzymes An enzyme is a protein used to speed up the rate of a chemical reaction. Because they regulate the rate of chemical reactions‚ they are also called catalysts. There are many‚ many different types of enzymes‚ because for each chemical reaction that occurs‚ an enzyme specific to that reaction must be made. To act on a substrate‚ an enzyme must contain an active site. The active site is the area on the enzyme that allows the substrate and enzyme to fit together. The amino acids that are present
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