Experiment A1: Kinetics of the Reaction between Acetone and Iodine The key aim of this experiment was to determine the rate equation for the acid-catalysed iodination of acetone and to hence consider the insinuations of the mechanism of the rate equation obtained. The stoichiometric equation for the reaction between iodine and acetone is below‚ followed by the rate equation (where x‚y‚z and k are the values to be obtained): I2 + CH3COCH3 CH3COCH2I + HI -d[I2]/dt = k [I2]x [CH3COCH3]y [H+]z
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INTRODUCTION Enzyme is a biological catalyst that acts on a molecule called substrate and it also significantly speed up a chemical reaction by lowering the activation energy. In order to learn about the enzyme and its behaviour‚ this lab practical is conducted to examine the kinetic of the enzyme alkaline phosphatase. As illustration‚ when alkaline phosphatase is added to a substrate called p-Nitrophenyl phosphate (colourless in alkaline solution)‚ a series of reaction takes place and eventually
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BIOLOGY LAB REPORT (UNIT 7: ENZYMES) GENERAL Enzymes are protein that acts as catalyst‚ lowering the activation energy need for reactions to progress in cells. The reaction can still occur without the presence of the enzyme‚ but at a much slower rate. The activation energy is the minimum amount of energy need for a chemical reaction to occur‚ yielding from a given set of reactants. In enzymatic reactions‚ we have substrates which are reactants of reaction bound to an enzyme. While an active site is
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------------------------------------------------- Enzyme Pre-Lab Harry Kang 9/26/12 1) The purpose of this lab was to determine the rate of enzyme activity under variety of different conditions‚ such as‚ different amount of drops of enzymes and different temperature of water. The class measured the pressure in the test tube during the reaction of the substance with‚ 1.5 ml of H2O2‚ 1.5ml of H2O and different amounts of enzyme drops‚ to determine how much oxygen gas is produced during the reaction
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LABORATORY REPORT (Click on the Save a Copy button on the panel above to save your report) Activity: Enzyme Activity Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 60 °C (140 °F) 3. Sucrase activity decreases with increasing sucrose concentration. Materials and Methods Effect of pH on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and fructose) produced 2. Independent Variable. pH 3. Controlled Variables
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EFFECT OF TEMPERATURE‚PH AND SUBSTRATE CONCENTRATION ON ENZYME ACTIVITY | Aim To investigate the effect of Temperature‚Ph and substrate concentration on the rate of enzyme activity. Hypothesis
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process to anabolic hormones of the human body. Intrinsically they have like outcomes on the human body to anabolic steroids‚ triggering fast weight and force advances‚ but of a minor scale cause of the frequency limiting consequence produced by the enzyme adaptation. However‚ this theoretical explanation is thought-out somewhat outdated due to the expansions which have been done in supplement science. Epistane is amongst the hottest upscale steroids obtainable today‚ and it is achieving attention very
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This experiment is to determine the unknown DNA plasmid using restriction enzymes and conducting electrophoresis finally comparing the resulting fragments with the known restriction map. In this lab‚ it succeeds in showing the fragments. In this report we will discuss the‚ results‚ limitations and possible errors. Introduction In biology restriction enzymes are used in several ways to modify and manipulate DNA molecules. One common use is to compare pieces of DNA from one that is unknown‚ with fragments
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In this enzyme lab‚ (insert creative name)‚ the enzyme catalase was observed under varying conditions in order to interpret what prohibits and inhibits the functioning of certain enzymes in chemical reactions. Enzymes aid in chemical reactions by speeding up the time it takes for the reaction to occur‚ without getting “used up” throughout the process. Catalase‚ the specific enzyme used in this lab‚ is a protein abundant in the liver and red blood cells. It enhances the breakdown of hydrogen peroxide
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this article‚ we will experiment on the significant in strength of the enzyme by using three different test tubes and measuring the amount of product they give off. To determine this we are going to test the amount of color absorbance by using a special tool to help us understand our results. We will see how our end results show the effect of the amount of concentration we apply to each test tube. The results would be shown by the support of two graphs. Introduction: Enzymes are highly important
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