Chemistry 512 Enzyme Catalysis Lab Report Pre-lab Questions: 1. Write a balanced chemical equation with state symbols for the reaction catalyzed by peroxidase. 2H2O2 2H2O + O2 (4H1 4O) (4H + 2O + 2O) 2. What is the substrate(s) of this reaction? What is the catalyst? Substrate = H2O2 hydrogen peroxide Catalyst = peroxide 3. At what approximate temperature do enzymes normally operate in the body of a warm-blooded animal? Would your answer change if the enzyme came from a plant or yeast? Enzymes normally
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Purpose: Restriction enzymes cut DNA at a certain palindromic sequence. Three samples of lamda DNA set up to be cut with restriction enzymes PstI‚ EcoRI‚ or HindDIII. There were also two more samples‚ one of these samples was not mixed with any restriction enzyme and the other was a marker‚ which used an enzyme which creates fragments with a known number of base pairs used to create a standard curve. All five samples were put through agarose gel electrophoresis in order to estimate the amount of
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Another postulate of the kinetic molecular theory is that gas particles are always in motion‚ like the other states of matter. But they are different in that they undergo random translational movement. In solids‚ the particles mainly experience vibrational motion and in liquids they mainly vibrate and rotate‚ with some translational motion. Gas particles move rapidly in straight lines‚ unless acted upon by another particle or the walls of a container. This continuous contact with the container leads
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Six Factors Affecting the Performance of an Enzyme Enzymes are considered catalysts; substances that increase the rates of reactions. Enzymes are responsible for thousands of metabolic processes that involve the sustainment of life‚ one common one is simple food digestion. Without enzymes‚ digestion would occur too slowly for life to continue. Enzymes maintain a protein structure consisting of one or possibly more than one polypeptide chains of defined primary structure‚ and take up a characteristic
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of pH on Peroxidase Enzyme Claire Bell Biology 1130 Jazmen Myers Section 36 Abstract Peroxidase is a turnip enzyme; it is used in the oxidation of hydrogen peroxide to lower activation energy‚ speeding up the reaction. The activity of peroxidase is highly dependent on its environment and most importantly the pH level. Peroxidase has been the focus of many recent studies and is believed to possibly reduce swelling among other things. We conducted an experiment testing the effect
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Chemistry 12: Reaction Kinetics Review 1. Looking at the expressions for reaction rate‚ write expressions with which you could express rates for the following reactions. (Hint: look at what happens to reactants and products.) Recall that solid or liquids can lose or gain mass‚ gases can lose or gain volume and aqueous solutions can increase or decrease in concentration. ("a" is done as an example.) a) Mg(s) + 2HCl(aq) H2(g) + MgCl2(aq) reaction rate = mass of Mg consumed unit time b) c) or
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An experiment was run to determine which enzyme (pectinase‚ and cellulase or combinations of the two enzymes) maximizes juice production and would be most cost effective. The proposed hypothesis was if the enzyme‚ pectinase‚ is added to apple juice‚ then the more juice will be extracted than if cellulase were added because pectinase holds the cell wall together and if it is separated apart from each other‚ then the more juice would be able to flow out. The experimental data show that during the ten
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Running head: LAB 5: INVESTIGATING AN ENZYME-CATALYZED REACTION Lab 5: Investigating an Enzyme-Catalyzed Reaction September 24‚ 2014 Principles of Biology 120.601 Mrs. Annemarie Duncan Abstract: (Burmania) This experiment was performed in order to examine ways in which a potato catalase enzyme reacts to various assays with differing variables. To do so a baseline assay (undiluted extract and room temperature H2O2) was used within the experiment with only one other variable changed in
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The milgram experiment. The three people involved were: the one running the experiment‚ the subject of the experiment a volunteer‚ and a person pretending to be a volunteer. These three persons fill three distinct roles: the Experimenter an authoritative role‚ the Teacher a role intended to obey the orders of the Experimenter‚ and the Learner the recipient of stimulus from the Teacher. The subject and the actor both drew slips of paper to determine their roles‚ but unknown to the subject‚ both slips
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15th Influence of pH on Enzyme Activities How does the pH of solution influence the speed of enzyme activities? Enzymes are protein catalysts and chemical that speed up chemical reaction without being involved in the reaction. They are involved in the human digestive system to help people digest food more efficient. Food are substrates to be break down and absorb through the digestive system in the body. Substrate is a molecule that bond with the active site of enzyme to transfer in to one or
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