Title: Enzyme Activity Lab Purpose: To measure the rate of enzyme activity from a tissue abstract and experiment with different factors‚ such as the enzyme solution and the substrate with different hydrogen peroxide percentages and temperature‚ that affect enzyme activity. Hypothesis: 1) If the disk is placed into each beaker with 100 units/ml of enzyme solution‚ then the time for the disk to float will be 30 seconds. 2) If the temperature of the solution is at 5 degrees Celsius‚
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GROUP 2 Kinetics of De-esterification for Synthesis of Benzoic Acid BATCH REACTOR Shane Bulk Chris Crosley David McGuire Max Skula Yunjing Song Shriram Sundarraj Nelson Zhou 155:416 Process Laboratory II Professor Jerry Sheinbeim January 28 – February 28‚ 2014 ABSTRACT The observed reaction that took place in this experiment was the de-esterification of ethyl benzoate to form benzoic acid. This experiment was used to determine the rate constant k of the synthesis of benzoic
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Introduction Enzymes are catalytic proteins. The purpose of a catalyst is to speed up metabolic reactions by lowering the free energy of activation or activation energy. Activation energy is known as the amount of energy needed to push the reactants over an energy barrier‚ so that the downhill part of the reaction can begin (Campbell 151). In an enzyme catalyzed reaction‚ the enzyme binds to its substrate‚ which is the reactant an enzyme acts on. In the reactions‚ the enzymes are very specific
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Abstract An experiment is conducted to prepare soap and thus‚ to compare the properties of the prepared soap and synthetic detergents which are precipitation‚ emulsification and cleaning abilities. It can be concluded that soap has the properties if emulsifying oil whereas detergent has not. The abilities of forming precipitates can be seen clearly in soap solution whereas detergent forms no precipitates at all. The experiment is completed and successfully conducted. Introduction
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Effects of Substrate Concentration‚ Reaction Time and Enzyme Concentration on Enzyme Reactions Corey von Ellm-St. Croix Rachael Kwan ID#: 20427841 Matthew Hrycyshyn & Saeideh Mayanloo Biol 130L‚ Section 017 Wednesday‚ 9:30am-12:20pm‚ 151 November 09‚ 2011 A living system controls its activity through enzymes. Enzymes are made from hundreds or even thousands of amino acids connected in a very unique and specific order. Almost all enzymes are proteins‚ except for ribozymes. The chain of amino
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I. Title. Restriction Enzyme Mapping of pBR322 Using Agarose Gel Electrophoresis. II. Authors. Author: Partner: Section: Thursday‚ 1:10 pm Date of Experiment: October 25‚ 2012 III. Introduction. Restriction enzymes (or restriction endonucleases)‚ originally isolated from Haemophilus influenzae in 1970‚ are enzymes within a cell that cleave foreign DNA within a specific and predictable nucleotide sequence (known as a restriction site) regardless of the source of such DNA. Such restriction
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Activity of the Enzyme Catalase in breaking down Hydrogen Peroxide and the effect of various factors on Enzyme Activity Introduction The enzyme catalase is present in cells in order to speed the breakdown of hydrogen peroxide (H2O2)‚ which is a toxic chemical to the human body. When hydrogen peroxide is broken down‚ the end products are Water (H2O) and Oxygen (O2). In this report‚ the reaction of catalase to hydrogen peroxide is being tested. Furthermore‚ the effects of temperature‚ concentration
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Enzymes are generally protein macromolecules that act as catalysts in metabolic reactions. A catalyst is a chemical agent that speeds up a reaction without being consumed by the reaction. Enzymes speed up metabolic reaction rates by lowering the activation energy barrier‚ which is the amount of energy initially needed to spark a reaction. It allows reactant molecules to absorb enough energy to break bonds and react without raising the temperature to an extreme. During this process the substrate
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Centre Number Surname Other Names Candidate Signature Candidate Number For Examiner’s Use Examiner’s Initials Question Mark General Certificate of Education Advanced Level Examination January 2011 1 2 3 Chemistry Unit 4 Kinetics‚ Equilibria and Organic Chemistry 9.00 am to 10.45 am Wednesday 26 January 2011 CHEM4 4 5 6 7 For this paper you must have: ● the Periodic Table/Data Sheet‚ provided as an insert (enclosed) ● a calculator. TOTAL Time allowed ● 1 hour
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Monica Perez Experiment 8: Acetylsalicylic Acid Experiment 01/25/2013 CHM Lab- 2211 Sec 0016 Instructor: Jennifer Reed Introduction: Commonly used as Aspirin‚ acetylsalicylic acid is an analgesic (pain reliever)‚ which is one of the products of the esterification reaction between salicylic acid and acetic anhydride. This esterification occurs since the hydroxyl group from the salicylic acid reacts with acetic anhydride to form an ester. In this experiment‚ we will be able to recreate
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