pH & Enzyme Action Aim: To inspect the effects of the pH on enzymes. Apparatus: 100 cm³ Beaker 3 – 5cm³ Syringes 2 Test Tube Racks with 8 Test Tubes Stop-watch Ruler Dropping bottle of detergent Marker Pen Masking Tape 400cm³ Hydrogen Peroxide 200cm³ Liver Catalase Solution 100cm³ of following Buffer Solution – pH5 pH7 pH9 pH11 Method: The materials were collected. The test tube rack one with 4 test tubes had been labelled A to D. The 2cm³ of each buffer solution
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.Potential energy and kinetic is what makes the roller coaster ascends up the hill and it descends Roller coasters the change of ds down the hill. Potential and kinetic energy is what makes the roller coaster move. What some people may not realize is that the roller coaster has no engine. When you’re going down hill they use different types of wheels to keep the ride smooth. A roller coaster is not pulled by cable. The 3 types of wheels are running wheels which guides the roller coaster‚ Friction
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Introduction: The purpose of this lab was to measure the extent of enzyme reaction on given substrates by means of color change. The reaction followed is given below: Tyrosinase³ Enzyme Pyrocatechol Hydroxyquinone Oxidation/Reduction Pink ³ Brown E+S + [ES] = E+P Enzyme Reaction Hypothesis: If there is an increase in enzyme concentration‚ an increase in reaction temperature‚ or an increase in buffer pH‚ then greater
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Catalase Lab Introduction: Catalase is an enzyme normally found in many plant and animal tissues. Its purpose is to destroy toxic substances which may be introduced into cells. Also‚ some cells use catalase to destroy cellular debris or worn out organelles. In this lab‚ we will use a catalase solution from potatoes and determine the effect of temperature and pH on the action of this enzyme. The substrate of the enzyme will be 3% hydrogen peroxide (H2O2). Catalase works by the following mechanism:
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Biology‚ Section G 27 September 2013 Experiment 5.3 Title: The Fragility of an Enzyme Purpose: To see how easily enzyme function can be destroyed. Hypothesis: I think that the bowl with only Jell-O will set as will the bowl with heated pineapple but the Jell-O with fresh‚ uncooked pineapple will not set. Materials: Part of a fresh pineapple A blender or a fine cheese grater 3 small bowls A small box of Jell-O Pot Stove Refrigerator 2 tablespoons Procedure: 1. Cut the pineapple
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13/12/2011 Course Title: General and Inorganic Chemistry TOPIC 3: CHEMICAL KINETICS Lecturer and contacts Mr. Vincent Madadi Department of Chemistry‚ University of Nairobi P. O. Box 30197-00100‚ Nairobi‚ Kenya Chemistry Dept. Rm 114 Tel: 4446138 ext 2185 Email: vmadadi@uonbi.ac.ke‚ madadivin2002@yahoo.com Website: http://www.uonbi.ac.ke/staff/vmadadi 12/13/2011 mov 1 Introduction • Kinetics is the study of rates of chemical reactions and the mechanisms by which they occur. • The reaction
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that the enzyme concentration will affect the rate of reaction but only up until a certain point because there is a limited supply of substrate. Enzymes are protein molecule that acts as biological catalyst by increasing the rate of reactions without changing the overall process. They are long chain amino acids bound together by peptide bonds. Enzymes are seen in all living cells and controlling the metabolic processes in which they converted nutrients into energy and new cells. Enzymes also help
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Enzymes are organic catalysts; where a substance speeds up the rate of chemical reactions without changing being changed by the reactions. In lab they tested this by seeing how H2O2 and the catalysts from the banana and liver react to make H2O+O2. Depending on different conditions; like decomposition of H2O2 (surface area)‚ temperature on function‚ reusing the catalase‚ reaction rate of iced liver returned to room temperature‚ and effects of pH on enzyme activity‚ to see how much O2 was released
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Enzyme Controlled Reactions 1) Describe the relationship between substrate concentration and the initial reaction rate of an enzyme-catalyzed reaction. Is this a linear relationship? What happens to the initial reaction rate as substrate concentration increases? A) The relationship between the substrate concentration and the initial reaction of an enzyme-catalyzed reaction is very productive‚ but is dramatically affected by the pH level of the given solution. The most productive pH level is
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placing newspaper onto the bench followed by the cutting tile on top. The liver was then cut into 1 cm cubes. 3) A 1 cm cube of liver was placed inside a 250mL measuring burette‚ we then added 9mL of a select concentrate of hydrogen peroxide and using the teat pipette we then added 1 mL of detergent and a drop of water. The timer was started. 4) The timer was stopped after two minutes had passed and then the results of how many mL of foam were produced were taken down. 10 mL was subtracted from
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