Enzyme kinetics Purpose: The goal of this investigation was to measure the amounts of products made and see the different elements that that affect the rate of breakdown of p-Nitro phenol in the absence or presence of cellobiase….. Methods: Activity #1 The materials used for this activity are as follows: 1.5 mM substrate‚ enzyme‚ Stop solution‚ buffer‚ DPTPs‚ 15 ml conical tubes‚ cuvettes‚ marker‚ beaker‚ distilled water‚ spectrophotometer‚ stop watch. First four 15 ml conical tubes
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Lab #5-Enzymes NAME DATE LAB PERIOD Introduction Enzymes are proteins‚ though highly complex and diverse‚ they serve one basic function; to work as an organic catalyst. A catalyst‚ as defined by Merriam-Webster dictionary‚ is a substance that enables a chemical reaction to proceed at a usually faster rate ("Catalyst-Definition and more."). They function by reducing the activation energy‚ or energy required to start a reaction. The way enzymatic reaction works cannot be altered‚ but the
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(Click on the Save a Copy button on the panel above to save your report) Activity: Name: Instructor: Date: Enzyme Activity cheryl yelton November 30‚ 2014 Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 40 °C (104 °F) 3. Sucrase activity increases with increasing sucrose concentration. Materials and Methods Effect of pH on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and fructose) produced 2. Independent Variable
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Enzymes are important components of life‚ facilitating reactions that are necessary for an organism to live. Enzymes can be very specific to what environment they function best in1. Numerous environmental impacts were tested for the enzyme peroxidase which catalyzes the decomposition of hydrogen peroxide. The basic decomposition reaction was carried out first without any environmental alterations. The hypothesis for this reaction was supported. The enzyme caused the amount of absorbance increase
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Report Worksheet (4 pages max.) Experiment title: How Do We Identify an Unknown Substance? Guiding questions: State the question or questions that that you were trying to answer in this lab. How do you determine the two unknown types of plastic? What are the two unknown plastics? Safety considerations: Briefly describe the safety measures you had to take to perform the experiments. Before entering the lab‚ I put on my lab coat and practiced using my goggles in case of any spills
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CHEMICAL KINETICS 1Y.S. MATBA 1Department of Materials‚ Mining‚ and Metallurgical Engineering‚ College of Engineering Date Performed: December 3‚ 2013 Date Submitted: December 09‚
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Environmental Science The Scientific Method Amber Steadham October 31‚ 2012 Abstract: We conducted several experiments using the Scientific Method. We made observations‚ recorded them and used our observations to propose a hypothesis. The experiments included chemical and physical reactions dealing with torn news paper‚ appearance of rock salt crystals‚ the appearance of sand‚ oil and water on plastic‚ and making iodine in water and mineral oil. We found that all these included four physical
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Experiment Plan By: Ashley Project Question: What size of bouncy ball bounces the highest‚ small‚ medium‚ or large? * I predict the following will happen (my hypothesis): I predict that the smallest ball will bounce the highest since it is the lightest. | * Independent Variable: I will be changing the ball size. * Dependent Variable: The height of the bounce. * Controlled Variable: The height that I will drop the balls at. | The materials and amounts of each that is
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Title Antimicrobial properties in different type of plants. Introduction A substance that kills or prevents the growth of microorganisms for example bacteria‚ fungi or protozoans is called an antimicrobial. This substance has 2 major roles which are to either kill microbes (microbiocidal) or prevent the growth of microbes (microbiostatic). Disinfectants are antimicrobial substances used on non-living objects outside the body. This substance included antibiotics‚ antifungals‚ antiprotozoals and
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amount of heat gained or lost in a system. Although this is not completely true‚ they are treated as isolated systems. A simple coffee-cup calorimeter can be constructed using two Styrofoam cups nested inside each other. The Styrofoam prevents heat loss to the surroundings‚ which makes it an ideal calorimeter.
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