INTRODUCTION Enzyme is a biological catalyst that acts on a molecule called substrate and it also significantly speed up a chemical reaction by lowering the activation energy. In order to learn about the enzyme and its behaviour‚ this lab practical is conducted to examine the kinetic of the enzyme alkaline phosphatase. As illustration‚ when alkaline phosphatase is added to a substrate called p-Nitrophenyl phosphate (colourless in alkaline solution)‚ a series of reaction takes place and eventually
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Experiment A1: Kinetics of the Reaction between Acetone and Iodine The key aim of this experiment was to determine the rate equation for the acid-catalysed iodination of acetone and to hence consider the insinuations of the mechanism of the rate equation obtained. The stoichiometric equation for the reaction between iodine and acetone is below‚ followed by the rate equation (where x‚y‚z and k are the values to be obtained): I2 + CH3COCH3 CH3COCH2I + HI -d[I2]/dt = k [I2]x [CH3COCH3]y [H+]z
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Simple Experiments on the Enzyme Catalase Aim: The aim of this practical is to use three different techniques to investigate the effect of different concentrations of the enzyme catalase on the rate of breakdown of hydrogen peroxide. Background information Catalase is an enzyme which is found in all living organisms. This enzyme catalases the decomposition of hydrogen peroxide into water and oxygen. Cells continually produce a poisonous by-product of metabolising‚ called hydrogen peroxide. This
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Laboratory III: Forces Problem IV: Normal and Kinetic Frictional Force I John Greavu March 13‚ 2013 Physics 1301W‚ Professor: Evan Frodermann‚ TA: Mark Pepin Abstract The dependence of two-dimensional velocity and position components on time as well as whether or not said components are constant was determined. Video was recorded of a ball being projected laterally and data of its flight were plotted. From analyzing the data and the corresponding graphs that followed‚ it was shown that while the
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it fun‚ exhilarating‚ and decently scary you also have to think about the scientific principles. As these will all be defined by Oxford’s dictionary. One of the most common scientific principles is kinetic energy. Kinetic energy is energy that an object possesses by virtue of being in motion. Kinetic energy increases as motion increases during the roller coaster ride. Potential energy is the energy of a body or a system with respect to the position of the body or the arrangement of particles in the
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Enzymes are responsible for many crucial processes in living cells since they accelerate chemical reactions which would occur too slowly‚ or would lead to different products without their contribution. Enzymes are biocatalysts that usually show high affinity to a specific substrate under particular environmental conditions. The binding of the substrate and catalysis take place at a specific small region‚ around 10 amino acids‚ in the enzyme known as active site which usually represents a hydrophobic
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The effect of enzyme concentration‚ substrate concentration‚ pH‚ and temperature on the enzyme catalase. Introduction: Enzymes are biological catalysts; proteins and RNA. They are required for most biological reactions and they are highly specific. Each enzyme has an active site. The active site is the spot on the enzyme where a substrate fits in. Substrates binds with enzymes through the active site. Enzymes‚ being highly specific‚ only fit with one certain substrate. Enzymes and substrates
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Lab report nº4 The aim of this experiment was to observe the change of enzyme reaction with different concentration of solution. For this experiment we used potato enzymes (catalase) and hydrogen peroxide in concentrations of 100%‚ 80%‚ 60%‚ 40%‚ and 20% According to P.George: “When catalase is added to hydrogen peroxide‚ there is an initial rapid evolution of oxygen which lasts for about two minutes‚ depending on the peroxide concentration. After this‚ oxygen is given off at a steady rate which
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Lab Report Effect of temperature on enzyme activity I. Purpose: Determine how effect of temperature on enzyme (catalyse) activity (in the liver). II. Materials * Raw liver * Forceps * 50mL 1% hydrogen Peroxide (H * 25mL graduated cylinder * 50mL‚ 400mL beakers * Pureed liver * 5 Filter-paper disks * Paper towels * Timer or stopwatch * Thermometer * Hot plate III. Procedure 1.Gather all necessary materials; start
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Effect of pH on Enzyme Activity A piece of Solanum tuberosum (potato) was removed and mixed with distilled water in a blender. The resulting solution was filtered through multiple layers of cheese cloth to filter out the liquid by eliminating any large pieces in the solution. The solution created was catechol. Five different solutions were prepared as blanks with each test tube containing 6.0mL of a different pH (pH 4‚ pH6‚ pH7‚ pH8‚ pH10) of phosphate buffer‚ 1.0mL of the enzyme and 1.0mL of water
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