May 1‚ 2013 Enzymes as Drug Targets Enzymes are defined as any of numerous proteins produced in living cells that accelerate or catalyze the metabolic processes of an organism. Enzymes are usually very selective in the molecules that they act upon‚ called substrates‚ often reacting with only a single substrate. The substrate binds to the enzyme at a location called the active site just before the reaction catalyzed by the enzyme takes place. Enzymes can speed up chemical reactions by up to
Premium Enzyme Metabolism Enzyme inhibitor
scale Enzyme applications 1. Detergents Bacterial proteinases are still the most important detergent enzymes. Lipases decompose fats into more water-soluble compounds. Amylases are used in detergents to remove starch based stains. 2. Starch hydrolysis and fructose production The use of starch degrading enzymes was the first large scale application of microbial enzymes in food industry. Mainly two enzymes carry out conversion of starch to glucose: alpha-amylase and fungal enzymes. Fructose
Premium Enzyme
Use the knowledge of kinetics to explain each of the following statements. a. An increase in temperature at which a reaction takes place causes an increase in reaction rate . An increase in temperature means increasing the energy of the molecules present. If the molecules has more energy ‚ then more of the them will collide often with enough energy ‚ to overcome the activation energy barrier. causing the reaction to proceed more quickly. b.An addition of the catalyst increases the rate at which
Free Chemical reaction Chemical kinetics
LABORATORY REPORT Activity: Enzyme Activity Name: Angela Collins Instructor: Catherine Rice Date: 07.09.2014 Predictions Sucrase will have the greatest activity at pH 5 Sucrase will have the greatest activity at 70 °C (158 °F) Sucrase activity increases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity Dependent Variable amount of product (glucose and fructose) produced Independent Variable pH Controlled Variables temperature‚ amount
Premium Enzyme PH Temperature
MISEP Chemistry 512 – Jacobs Enzyme Catalyst Lab - Formal Report – August 8‚ 2007 ABSTRACT This investigation examined what would happen to the rate of an enzyme-catalyzed reaction if the concentration of substrate changed. We hypothesized that if the concentration increased‚ then the reaction rate would also increase. To test our question‚ we varied a combination of substrate and buffer‚ totaling 6mL‚ with a constant amount of 2 drops of catalyst. The enzyme catalyst‚ peroxidase‚ increased
Premium Chemical reaction Enzyme Chemistry
structure of the enzyme is mainly dependent on the active site and variable groups. Extreme temperatures or extreme pHs can alter the structure of an enzyme. Enzymes function to lower the activation energy to break the bonds. They achieve this by putting stress and pressure on the bonds or creating a microenvironment for the substrate. Enzymes are regulated by inhibitors or activators and can be inhibited by the products of the reaction‚ called feedback inhibition. Enzymes are catalytic proteins;
Premium Enzyme Enzyme inhibitor Catalysis
Biochemistry Unit The Kinetics of Alkali Phosphatase Inhibition 1. OVERVIEW This practical builds on the enzymology lab skills you learned in the Acid Phosphatase practical. Again‚ you will measure the initial reaction velocity (V 0) of an enzyme reaction‚ but this time in the absence and then presence of an inhibitor. Last time you used Acid Phosphatase (Prac 1)‚ but this time you will use the enzyme Alkali Phosphatase. These enzymes have different primary (and hence tertiary) structures
Premium Enzyme PH
Kinetic and Thermodynamic Reaction Condition Brandon Bullock 2/10/15 Objective The purpose of this experiment is to test kinetic theory by running several reactions and determine which product are formed under kinetic conditions and which products are formed under thermodynamic conditions. Kinetically controlled products have low transition states. This makes it easy for the product to be formed at low temperatures
Premium
Study Guide for Unit V Energy and Enzymes. What is cellular work? Why must living things utilize energy with maximum efficiency? Define energy. Why do living things need a constant input of energy? Where does all energy in the biosphere originate? Define kinetic and potential energy. (Give biological examples). What are the two laws of thermodynamics? Give examples. What is entropy and what is the law of entropy? How do living things resist the law of entropy? Give biological examples. Define
Premium Metabolism Enzyme Adenosine triphosphate
on enzyme activity Objective To study the effect of two heavy metal ions‚ lead (II) ions and silver ions on the enzyme activity of invertase. Introduction Hypothesis: The rate of enzyme reaction of invertase decreases and the reaction eventually stops as the heavy metal ions inhibit the enzyme reactions. Biological principle: Heavy metal ions are non-competitive inhibitors. They do not compete with the substrates for the active sites of enzymes. They attach to other parts of the enzyme molecules
Premium Enzyme Silver