2014 Biology 1 Cellular Processes Lab Section 903 Tianna Clarke Materials and Methods Part I – Restriction Enzyme Digestion To begin this experiment‚ the DNA molecules must be cut into smaller fragments with distinct enzymes called Restriction Enzymes through a process called Restriction Enzyme Digestion. Four microtest tubes were labeled 1 through 4 and added 10 µl of Enzyme Reaction Buffer to each of the four reaction tubes using a micropipette. DNA‚ and Enzyme 1 and 2‚ were then added to the
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The Effect of pH on the Rate of Enzyme Catalysis of Catalase Objectives: The objective of this lab was to develop a protocol to investigate the effect of an environmental variable on the catalytic function of an enzyme. More specifically‚ the objective was to perform an experiment in order to test the effect of pH on the function of the enzyme catalase. Introduction: Enzymes are proteins that act as catalysts for reactions. This simply means that enzymes lower the activation energy required
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This lab is adapted from the canned denatured pineapple experiment. Instead of investigating the effect of canning on enzyme activity‚ we look for the presence of the substrate. This is a useful teaching lab for all the criteria and requires very few resources. Background Information: Gelatin is made from a protein called collagen which comes from the joints of animals. Gelatin may be dissolved in hot water. As the dissolved gelatin mixture cools‚ the collagen forms into a matrix that
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Part I - Introduction Enzymes are proteins that act as catalysts to regulate metabolism by selectively speeding up chemical reactions in the cell without being consumed during the process. During the catalytic action‚ the enzyme binds to the substrate – the reactant enzyme acts on – and forms an enzyme-substrate complex to convert the substrate into the product. Each type of enzyme combines with its specific substrate‚ which is recognized by the shape. In the enzymatic reaction‚ the initial rate
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Jennifer Anatomy and Physiology Enzyme Lab Report Introduction The chemical reaction that is being studied is the hydrolysis of starch. The enzyme that is being studied is amylase. This experiment is looking at the effect of temperature on the rate of the enzymatic hydrolysis of starch. My hypothesis is that the higher the temperature the faster the hydrolysis of the starch would occur. The rationale behind this is that heat
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the frequency and wavelength of each one. A high frequency‚ which is high energy‚ results in a short wavelengthand vice versa. This makes the frequency and wavelength inversely proportional. Infrared radiation has longer electromagnetic wavelengths than that of
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In this experiment‚ we used acquired observational data to develop an activity series describing the relative reactivity of the metals being tested. The elements within a periodic table often show different trends in physical and chemical properties depending on the period or group they are apart of. In a periodic table‚ elements are arranged by increasing atomic number in horizontal rows called periods. The atomic numbers represent the number of protons in each element. The periodic table is also
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intermediate copper compounds back into copper metal. By weighing the copper at the beginning and at the end of the experiment the percentage yield can be determined. Method: The experiment was carried out as outlined in the practical manual. Results: Table: showing masses: Mass copper wire 0.2510 Mass crucible 28.9257 Mass watch glass 19.6213 Mass watch glass + copper 19.7890 Mass copper product 0.1677 Calculations: Percentage yield: Discussion and conclusion:
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Each element emits a unique color light. The purpose of this experiment was to observe these unique colors and calculate their energy. In order to observe this‚ we placed the physical elements in a 24 well plate‚ dipped wet q-tips into the wells to gather the element‚ and put it over the bunsen burner to observe the flame emitted. While observing the different flames‚ we saw that different elements did indeed emit unique flame colors. Colors such as red‚ violet‚ and turquoise were emitted. All of
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Enzyme assay lab report Health and safety: 4-nitrophenol is harmful. Introduction: Enzymes are quaternary structured proteins that are specific biological catalysts that speed up a reaction without being used up. They contain an active site that allows substrate to bind to a specific area on the enzyme which is of a complimentary shape of the substrate. There are two models of enzyme action‚ the Lock and Key model and the Induced Fit model. The Lock and Key model states that the enzyme has a specific
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