In this lab we tested the effect of temperature has on the rate of enzyme activity. The way we figured this out was by taking four different temperatures and testing the difference absorbance levels they produced every 20 seconds for about 2 minutes straight using a spectrophotometer. The important part of this experiment was the temperature the enzyme concentration was made at. What we got from the experiment was at lower temperature we got very low numbers for the absorbance‚ which gave us a lower
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Enzymes Reactions to Changes in Substrate and Inhibitors Benjamin J. Mora Coronado University of Texas Rio Grande Valley at Edinburgh Abstract Purpose for the experiments was to test the enzymes in various scenarios and see how changing this would affect the rate of reaction. The enzyme source used in the experiments was Turnip Extract. Concentrations of Turnip extract for activity 1 where o.5ml‚ 1.0ml‚ and 2.0 ml as for the rest of the activities 2 Through 4 stayed at a consistent concentration
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AP/IB Chemistry Internal Assessment Lab Format The following titles and subtitles (in bold) should be used for your lab report and given in this order within your lab report. Title: choose one to fit your experiment I. Design A. Problem – must be a focused‚ clear research question. B. Hypothesis (When appropriate) • Clear answer to Problem – • Logical rational • your conclusion should address the hypothesis you are giving here. C. Variables
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Lab #5-Enzymes NAME DATE LAB PERIOD Introduction Enzymes are proteins‚ though highly complex and diverse‚ they serve one basic function; to work as an organic catalyst. A catalyst‚ as defined by Merriam-Webster dictionary‚ is a substance that enables a chemical reaction to proceed at a usually faster rate ("Catalyst-Definition and more."). They function by reducing the activation energy‚ or energy required to start a reaction. The way enzymatic reaction works cannot be altered‚ but the
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IB Biology Potato Lab Table 1: Trial Number | Concentration of Sucrose Solution (M) ±0.2 ml | Initial Mass of Potato Core Slice(g) ±0.1 | Final Mass of Potato Core Slices (g) ±0.1 | 1 | 0.0 | 7.7 | 9.3 | 2 | | 6.0 | 8.1 | 3 | | 6.2 | 7.4 | 4 | | 10.2 | 13.2 | 5 | | 8.7 | 10.3 | 6 | | 4.9 | 6.0 | 7 | | 9.2 | 10.4 | 1 | 0.2 | 5.8 | 6.0 | 2 | | 11.6 | 12.1 | 3 | | 2.5 | 3.1 | 1 | 0.4 | 14.4 | 13.9 | 2 | | 2.6 | 2.8 | 3 | | 8 | 6.5 | 1 | 0.6 | 7.3 | 5.3 | 2
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The objectives of this experiment were to investigate the activity of enzymes‚ components that influence the enzyme’s activity‚ identify an unknown phosphatase‚ influence of inhibitors‚ and determine if inhibition is competitive or noncompetitive. A spectrophotometer evaluated the measurement of color change over a period time due to product being formed. Determining unknown phosphatase and effects from different inhibitors were determined by varying the pH and substrate concentrations. The unknown
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Chemistry 512 Enzyme Catalysis Lab Report Pre-lab Questions: 1. Write a balanced chemical equation with state symbols for the reaction catalyzed by peroxidase. 2H2O2 2H2O + O2 (4H1 4O) (4H + 2O + 2O) 2. What is the substrate(s) of this reaction? What is the catalyst? Substrate = H2O2 hydrogen peroxide Catalyst = peroxide 3. At what approximate temperature do enzymes normally operate in the body of a warm-blooded animal? Would your answer change if the enzyme came from a plant or yeast? Enzymes normally
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regular hydrogen peroxide 1%‚ 25%‚ 50%‚ 75%‚ and 100% substrate concentration tweezers timer permanent marker pen/pencil paper for data PROCEDURE: The experiment begins with five beakers full of various substrate concentrations. To begin the lab‚ gather five plastic‚ Solo cups and label them 1%‚ 25%‚ 50%‚ 75%‚ and 100% with the permanent marker. Then‚ fill each cup with 75 mL of regular hydrogen peroxide. After‚ lightly dip the filter paper into the 1% substrate concentration till it is completely
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BIOL 1364 LABORATORY EXERCISE 3 ________________________________________________________________________ INHERITANCE OF CHARACTERS Objectives of the Laboratory: i) Determination of the genetic control of seed colour‚ ie how many genes control seed colour‚ what sort of intra allelic (dominance relationship) and interallelic interactions (independent assortment vs epistasis) govern the inheritance of seed colour. ii) Understand the scientific process involved in studying character
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concentration of substrate‚ on the activity of the enzymes. By conducting these three separate experiments also‚ three graphs are able to be obtained where the trend of each factor affecting on the enzyme activity is shown and described clearly. II. Hypothesis Experiment 1 (Effect of Temperature): As the temperature increases‚ the height of the bubble will increase too‚ indicating a faster rate of reaction. Experiment 2 (Effect of pH): Enzymes are affected by changes in pH. Extremely high or
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