Enzyme Kinetics Examples and Problems 1. An enzyme is produced for producing a sun protection lotion. Given kinetic data for the enzyme reaction with Vm=2.5 mmol/m3.s‚ Km=8.9 mM and So=12mM‚ what would be the time required for 95% conversion in a batch reactor? 2. An enzyme was assayed at an initial substrate concentration of 10-5M. The Km’ for the substrate is 2x10-3M. At the end of 1 min‚ 2% of the substrate had been converted to product. a. What % of the substrate will be converted
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Effect of temperature on enzyme activity In this experiment… Independent variable: Temperature of the amylase Dependent variable: Enzyme activity which is measured by the time for disappearance of starch Controlled variables: Volume of amylase; volume of starch solution; concentration of amylase; concentration of starch solution Prediction of results i) At low temperature‚ the rate of amylase activity is very low. ii) At optimum (=best) temperature‚ the rate of amylase activity is the
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Simple Experiments on the Enzyme Catalase Aim: The aim of this practical is to use three different techniques to investigate the effect of different concentrations of the enzyme catalase on the rate of breakdown of hydrogen peroxide. Background information Catalase is an enzyme which is found in all living organisms. This enzyme catalases the decomposition of hydrogen peroxide into water and oxygen. Cells continually produce a poisonous by-product of metabolising‚ called hydrogen peroxide. This
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Outlining an IA for IB Economics Plan: I will be only talking about the first part of the article “ China commodity imports soar despite high costs” Identify economic concepts (vocabulary words) that explain content of article Demand for oil and copper Purchases of other commodities imports of unwrought materials raise retail fuel prices China’s crude imports will remain high in the upcoming months as oil firms still have a strong incentive to produce raise crude throughput to a new peak
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p IB DIPLOMA PROGRAMME PROGRAMME DU DIPLÔME DU BI PROGRAMA DEL DIPLOMA DEL BI Biology Higher level and standard level Specimen paper 1s‚ 2s and 3s For first examinations in 2009 http://www.xtremepapers.net CONTENTS Biology higher level paper 1 specimen paper Biology higher level paper 1 specimen markscheme Biology higher level paper 2 specimen paper Biology higher level paper 2 specimen markscheme Biology higher level paper 3 specimen paper Biology higher level paper 3 specimen markscheme
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taking a simple test. This paper will evaluate Loftus and Palmer’s (1974) Lab Experiment 2 and Levinger & Clark (1961) Experiment‚ which tested the extent to which memory is reliable. Both studies point out that memory can be manipulated and differs depending on the subject and time‚ making it less reliable than what an individual would initially expect. Firstly‚ this paper will look into Loftus and Palmer’s (1974) Lab Experiment 2. The aim of this experiment was to see whether misleading post-event
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Temperature Affecting Enzyme Activity Introduction The basic properties of life revolve around chemical reactions. Without the presence of enzymes some of life’s processes would not come so easily. Enzymes are basically proteins‚ which have specific shapes for different substrates. Enzymes change the rate in chemical reactions. It does this without having to change its own shape‚ which makes enzymes different from other proteins. A common enzyme that we have is catalase‚ which breaks down
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cheese. Lactic acid bacteria(LAB)‚ a bacteria that can be found in the production of cheese‚ its stress gene was investigated in the experiment by using various biochemical and genetic techniques to identify and extract. The characterisation of the strain illustrates how identification of strains differ using different methods‚ such as gram stain and 16s rRNA screening. After the characterisation‚ the stress gene isolation assist the further understanding of the gene on LAB be giving different stress
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The Effect of Enzymes on Apple Juice Production Biology Period 6 Background Info The purpose of this experiment was to determine which enzyme or the combination of the two enzymes makes the most apple juice from applesauce. We did his by‚ mixing 10 drops of an enzyme (cellulose/pectinase/both) into a few tablespoons of applesauce. We then left the rest to nature‚ and watched the liquid funnel out for 10 minutes. Lastly‚ we recorded our data. The cell wall is a complicated
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References: * Alkhinji‚ A.‚ 2011. Measuring DNA concentration with the Nanodrop Spectrophotometer. Fero Lab: Fred Hutchinson Cancer Research Center * Garrett‚ R.H.‚ Grisham‚ C.M.‚ 2010. Biochemistry‚ Ed 4. Brooks/Cole Cengage Learning * Snyder‚ L.‚ Champness‚ W.‚ 2007. Molecular genetics of bacteria‚ Ed 3. American society for microbiology * Varsani‚ A.‚ Heinemann‚ J.‚ Tisch‚ M.‚ 2013. BCHM231/202 S1 Lab Manual: Foundations in Molecular Biology. School of Biological Sciences. University of Canterbury
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