Horseradish Peroxidase Abstract There are several factors that can affect the rate of reaction of peroxidase such as temperature‚ pH‚ concentration of peroxidase present and whether or not it has been boiled. Our experimental data demonstrated that peroxidase activity peaked between 23 degrees Celsius and 32 degrees Celsius. We found the pH to be 7 for optimal activity. As far as the concentration is concerned our results showed that as the concentration of horseradish peroxidase doubled so
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Ayaan Nadeem SBI 4UO-A Tuesday‚ February 27‚ 2013 Ms. Balmer Pineapple Jelly Enzyme Lab Discussion After completing the Pineapple Jell-O Enzyme lab‚ the final results were that the canned pineapple formed the jelly while the fresh pineapple did not. Pineapple In order for this to have occurred‚ there has to be a comparison between fresh and canned pineapple in terms of their physical and chemical properties. The physical properties of fresh pineapple are that it is sweet‚ ripe and raw.
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regular hydrogen peroxide 1%‚ 25%‚ 50%‚ 75%‚ and 100% substrate concentration tweezers timer permanent marker pen/pencil paper for data PROCEDURE: The experiment begins with five beakers full of various substrate concentrations. To begin the lab‚ gather five plastic‚ Solo cups and label them 1%‚ 25%‚ 50%‚ 75%‚ and 100% with the permanent marker. Then‚ fill each cup with 75 mL of regular hydrogen peroxide. After‚ lightly dip the filter paper into the 1% substrate concentration till it is completely
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Chemistry Unit 1 – Revision Questions Chemistry 1A 1) Define an element. How many are there in the Periodic Table? 2) Write a table with the charges and masses of protons‚ neutrons and electrons. 3) Define atomic number and mass number. 4) Draw electron (energy level) diagrams for Be‚ S‚ Al‚ Cl and K. Also‚ write down the number of protons and neutrons for each. Finally‚ write the symbols (with mass no. and atomic no.) 5) Why have all Group 7 elements (halogens) got similar chemical properties
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1.7 Factors that affect the activity of an enzyme It is important when working with enzymes to understand basic enzymatic theory behind them when selecting conditions to measure the activity of the enzymes. The factors that are known to affect the concentration of enzymes are temperature‚ pH‚ concentration of enzyme‚ concentration of substrate‚ buffer type and concentration‚ the presence of any inhibitors and cofactors (Worthington-biochem.com). 1.7.1. Temperature With most catalysed reactions‚
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NaCl Effects on Peroxidase Activity My experiment was to see if adding NaCl to solution would have any effects on peroxidase activity. The materials that were used in this experiment were pH 7 buffer(DI water)‚ peroxidase‚ NaCl‚ guaiacol and hydrogen peroxide; added in that order. Blanks were created for each NaCl concentration‚ 0%‚ 5%‚ 7.5% and 10%. Each cuvette had .5ml of pH 7 buffer‚ 1ml of peroxidase‚ .02ml guaiacol for the experimental cuvettes and 0ml of guaiacol for the blank cuvettes‚
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Environment Can Effect Enzymes Introduction: In a chemical reaction there sometimes can be a catalyst present known as an enzyme. An enzyme is a protein that speeds up a chemical reaction by lowering the amount of activation energy required to start the reaction. By lowering the activation energy‚ more of the substrate is able to participate in the reaction‚ speeding it up. Enzymes are substrate specific. The substrate is what the enzyme bonds to. That is to say that enzyme A will only react with
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Introduction Enzymes are proteins that are involved in all the chemical processes in living things. As they are made of proteins they are affected by pH and temperature. Enzymes are catalysts; they speed up chemical reactions without being changed themselves. Digestive enzymes speed up the breakdown of large food molecules into smaller ones so that the blood can absorb them. Enzymes turn a large starch molecule into thousands of tiny glucose molecules. Enzymes end in ’ase’. There are thousands of
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Lab Report: Toothpickase and Enzymes September 19‚ 2012 Olivia DePhillips Lab Partner: Joy Morgan Meyers Signature: Introduction Enzymes are biological molecules that catalyze chemical reactions. In enzymatic reactions‚ the molecules at the beginning of the process‚ called substrates‚ are converted into different molecules‚ called products. Almost all chemical reactions in a biological cell need enzymes in order to occur at rates sufficient for life. Like all catalysts‚ enzymes work
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Lab #4: The Immune System Purpose: The purpose of this lab was to perform and understand the procedures of conducting an ELISA test to determine whether a particular antibody is present in a patient’s blood sample through a virtual simulation. Hypothesis: If I successfully complete this lab‚ I will then understand how to perform an ELISA test‚ the purpose an ELISA test‚ and also how to interpret the results of this test. Materials and Procedures: Materials: Howard Huges medical
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