expressed‚ rate of degradation‚ modifications‚ how they interact with other proteins and more. The goal in this lab was to determine the similarities and differences in muscle proteins among 5 species of fish using SDS-PAGE and Western blotting. First‚ proteins are separated based on size. Second‚ antibodies are used to detect the protein of interest. Lastly‚ a substrate that responds with an enzyme is used to view the antibody/protein complex. Within a muscle tissue‚ there could be as many as 19 different
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bleaches and cleansing wounds. In a cell its build up would be highly toxic. However‚ liver cells contain an enzyme‚ catalase‚ which immediately breaks down hydrogen peroxide. It is a peroxidase and breaks up the toxic hydrogen peroxide to water and oxygen which are both nontoxic. The reaction is exothermic‚ meaning that energy is released in the form of heat. It is the fastest known enzyme. Hydrogen peroxide Water + Oxygen 2H2O2 2H2O O2 List of materials: Safety glasses Iron powder in a watch
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The mean data observed show a number of trends. The average weight and length tend to decrease as the amount of Ammonium Sulfate approaches the full recommended dose. On the other hand‚ the average amount of protein per gram of tissue tends to increase as the amount of Ammonium Sulfate approaches the full recommended dose. Our null hypothesis was that there is no difference between the mean weights or the mean lengths of the seedlings two different treatments. (A+B‚ A+C‚ and B+C) Each of these
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6 November 2012 SUBJECT: Lab Report Analysis This memo proposes the observations and claims I collected from reviewing three different lab reports. Three fields of study are composed within this memo that includes Electrical Engineering‚ Environmental Engineering‚ and Petroleum Engineering. “Electrical Filters‚” (Electrical)‚ written by Joe Schmoe‚ is a lab report made by a student at a university. The College Board produced an environmental lab report named‚ “Monitoring Air Quality‚”
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The temperature of water was determined by a thermometer. 1 cm stripped ends of the wires was hooked into the input posts on the back of the impendence converter. Lab tutor module was started after. B. Baseline heart rate The top of the container was covered with foil and the animal was allowed to sit quietly for 10 min. The trace was examined by pressing start. After that the baseline or the controlled
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the acyl-enzyme is then hydrolysed by nucleophilic attack of serine on the peptide carbonyl group‚ collapse of the tetrahedral intermediate and the release of the amine component. Histidine 57 then acts as a general acid catalyst and draws a proton away from the water molecule. The OH- ion then attacks the carbonyl carbon atom of the acyl group which forms another tetrahedral intermediate which breaks down to form the carboxylic acid product. The release of this acid then frees up the enzyme to continue
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Gbengon‚Sennie Microbiology Lab Case study # 5 November 1‚2012 1. What evidence suggests that the young graduate student has contracted what disease? Ans: The signs and symptoms the young graduate student presented‚ show evidence of Lyme disease. 2. What is the environmental pathway for the vector of this disease? Ans: The environmental pathway for the vector of this disease would be a forested habitat. The black-legged tick or the deer tick is the principle vector of this disease (Lyme
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Biology 160 Lab Manual Tacoma Community College Last update 06/03/2013 This page left intentionally blank Table of Contents Table of Contents i Laboratory 1: Principles of the Scientific Method 4 In-Lab Report Sheet 12 This page has been left intentionally blank 27 Laboratory 2: Scientific Methodology & Enzyme Activity 28 In-Lab Report Sheet 28 Objectives 33 Introduction 33 Parts of the Swift M5 Microscope 33
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SCIE211 Phase 2 Lab Report Title: Speciation Instructions: You will need to write a 1-page lab report using the scientific method to answer the following question: • What would happen if a species within a population were suddenly split into 2 groups by an earthquake that creates a physical barrier like a canyon? When your lab report is complete‚ post it in Submitted Assignment files. Part I: Use the animated time progression of speciation to help you write up your lab report. Part II: Write
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The Catalase Lab Stephen Human Anatomy & Physiology 9/30/12 Problem- How do different environments affect the reactivity of catalase? Hypothesis- If more catalase is added then more oxygen (kPa) will be produced in a faster rate because there is more catalase to react upon. If less catalase is added then less oxygen (kPa) will be produced in a slower rate because there is less catalase to react upon. Variable- Independent- Amount of Catalase (Filter Paper) Dependent- Amount of
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