Measurement of Capacitance using Arduino – An Interrupt-based Approach: Part I SAIKAT PATRA‚ SHIBENDU MAHATA Introduction Capacitive sensors are widely employed to measure various physical and chemical process parameters such as displacement‚ acceleration‚ thickness‚ force‚ pressure‚ stress‚ level‚ and humidity. The measured value of capacitance is then calibrated in terms of the process parameter for indication and/or control applications. This project presents an interrupt-based approach by
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Protocol for Lab 5 – Aerobic Respiration Part 1 Isolation of Mitochondria from Cauliflower - Weigh 50g of rosettes cut from fresh cauliflower head. - Cut rosettes and place it on ice - Prepare juice extractor by placing ice and an empty 150 ml beaker into the right compartment. - Collect pulp from left compartment and record total volume of the extract. Approx. 20ml - Filter the pulp using six layered cheese cloth and collect it in a beaker sitting on ice. - Place two 50 ml test tubes
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the acyl-enzyme is then hydrolysed by nucleophilic attack of serine on the peptide carbonyl group‚ collapse of the tetrahedral intermediate and the release of the amine component. Histidine 57 then acts as a general acid catalyst and draws a proton away from the water molecule. The OH- ion then attacks the carbonyl carbon atom of the acyl group which forms another tetrahedral intermediate which breaks down to form the carboxylic acid product. The release of this acid then frees up the enzyme to continue
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--------------------------------------------------------------------------------------------------------------------------------------- Abstract The interactions of Mg2+ and Zn2+ ions in the activation of non-specific tissue alkaline phosphatase were investigated using crude extracts of rat kidney. Activation of alkaline phosphatase by the metal ions was accompanied by changes in the kinetic parameters of pnitrophenylphosphate hydrolysis. The results suggest some synergistic interactions between Mg2+ and Zn2+ ions
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Effect of enzyme concentration on rate of reaction Research question: is there a relationship between catalase concentration and the rate of reaction? Hypotheses: if the enzyme concentration is increased then the rate of reaction will increase. If the enzyme concentration is decreased then the rate of reaction will decrease. Controlled: temperature Independent: % catalyze concentration ( Dependent: rate of reaction (mm/s) Materials: Mortar and pestle Graduated cylinder Distilled
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Processes Lab Section 903 Tianna Clarke Materials and Methods Part I – Restriction Enzyme Digestion To begin this experiment‚ the DNA molecules must be cut into smaller fragments with distinct enzymes called Restriction Enzymes through a process called Restriction Enzyme Digestion. Four microtest tubes were labeled 1 through 4 and added 10 µl of Enzyme Reaction Buffer to each of the four reaction tubes using a micropipette. DNA‚ and Enzyme 1 and 2‚ were then added to the reaction tubes using a new
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Introduction The aims of the lab session was to familiarize with the principles‚ operation and application of a light spectrophotometer. This report discusses an experiment to study the relationship of absorbance and concentration‚ the interaction of electromagnetic radiant energy(ERE) and matter which is an important aspect of the Beer-Lambert ’s Law. This law states that the absorption of a compound is directly proportional to the concentration of that particular compound. This is because (a &
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wavelengths and intensities of light. This lab will evaluate the optimal wavelengths and degrees of intensity during photosynthesis when chloroplast is exposed to light. The mixtures of DCPIP with water‚ PO4 buffer‚ and chloroplast will be prepared in a number of cuvettes. The cuvettes were tested individually at different wavelengths and intensities to find the optimal rate of photosynthesis by using a spectrophotometer‚ measuring the greatest change in absorbance. From this experiment‚ two data charts
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MEC 325/580 Lab Report CNC Machining Lab Report Date: March 18‚ 2013 Group Leader: Jian Wu Team Members: Group Number: G4 Regment No.: D Insturctor: CONTENTS 1.Objective and introduction. 2.Principles and practice. 3.Design and result. 4.Conclusion and recommendations. Appendices 1. Objective and introduction * Objectives Operate the CNC milling machine using G-codes‚ M-codes in a CNC program. * Equipment: Minitech CNC machine (Series 2) * Machine
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Abstract: In this Lab we used the chemical DPIP to detect the rate of succinate broken down by the mitochondrial solution. We detected the amount of DPIP in the solution with a spectrophotometer and measuring the absorbance of light at the 600nm range. DPIP is a useful chemical to use in this experiment because it goes from a blue color when oxidized to a colorless liquid (Ogura‚ 281)‚ this is due to the hydrogen ions and electrons released during the transitional step between succinate and fumarate
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