firstly amplified by PCR‚ then cloned in the pBluescript plasmid vector. EZH2 is an enzyme which adds methyl groups to the twenty seventh amino acid (Lysine) and is encoded by EZH2‚ the EZH2 gene encodes part of the Polycomb group which make protein complexes that help to maintain genes transcriptional repressive state over successive cell generations. http://www.ncbi.nlm.nih.gov/gene/2146 Throughout this lab report template DNA that contains the gene EZH2 was provided‚ this will be amplified by
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observe the effect of physical and chemical stress on the membrane permeability of beetroot. METHOD A large beetroot was washed with water. 14 cylinders were cut‚ all to the exact length (approx. 1cm with all the skin removed‚ including each ends) using a cork borer (approx. 10mm diameter). All of the beetroot cylinders were washed under running tap water for
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Solute Concentration of Potatoes Lab #1 Purpose: To find the molarity/concentration of potato cytoplasm. Materials: As on page 1 in the lab handout. Procedure: As on page 1 in the lab handout. Data and Observations: Test Tube # | Concentration of sucrose solution (mol/L) | Initial Mass (g) | Final Mass | Percentage change in mass | 1 | 1.0 mol/L | 3.00g | 2.25g | -25.0% | 2 | 0.9 mol/L | 2.70g | 2.07g | -23.3% | 3 | 0.8 mol/L | 2.92g | 2.25g | -22.9% | 4 | 0.7 mol/L | 2.60g
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Pablo Cuate Biochemistry Lab Report Objective: The purpose of this lab is to analyze enzyme activity at pH neutral for acid phosphatase as the colorless p-nitrophenylphosphate (PNPP) substrate concentration changes. We will empirically determine the concentration of production formation‚ Vmax and Km from absorbance of yellow PNP in the reaction. Results Figure 1 This figure indicates a positive linear trend for the concentration of PNP and absorbance. Table 1: Absorbance Value for Acid Phosphatase
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Abstract This lab’s purpose was to test the patterns of percolation in various places. This lab tested how soil would absorb water based on different places. Distance from a group of trees‚ distance from a swamp‚ and different types of soil were tested. From the results collected‚ you can not prove‚ but you can infer that the ability to absorb increases as you go farther away from a swamp‚ and a group of trees. The ability to absorb also increases based on how much silt is in soil. Introduction
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The Charge/Mass (e/m) Ratio of the Electron PHYS 0212: Introduction to Laboratory Physics Fall 2012 Abstract The experiment conducted demonstrated correlation between the charge and mass of an electron and the behavior of magnetic fields. The lab was divided into four parts. The first three parts were conducted with a compass that was used to locate the magnetic field lines around a bar magnet‚ a solenoid and then a pair of Helmholtz coils. As a result of these trials‚ our observations found
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influencing multiple (and possibly unrelated) phenotypic traits. Mutation in this type of gene will simultaneously affect more than one trait. An example is phenylketonuria‚ a human disease caused by mutation(s) in a single gene that codes for the enzyme‚ phenylalanine hydroxylase. The disease is characterized by mental retardation and reduced hair and skin pigmentation. List and explain other factors that
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volumetric flask it contained 8 mg/L benzoic acid + 10 mL HCl‚ then diluted to the mark on the flask with DI water. Standards of caffeine were also prepared that contained 4‚8‚12‚16 and 20 mg/L in 0.10M HCl. All other standards were prepared by other lab groups. These standards were used to obtained a series of calibration curves. We then prepared the monster energy drink‚ by warming 20 mL of the monster energy drink on a hot plate in order to expel CO2 and filtered the warm liquid through filter paper
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# Now You See It – Copper Cycle Lab The purpose of the lab is to discover what happens when someone executes a series of procedures‚ beginning with copper metal. What is done | What is observed | 1. Started with copper‚ Cu (s). | reddish‚ brownish‚ orange-ish‚ powder-like | 2. Added nitric acid‚ HNO3 (aq). | acid turns blue and smells like chlorine. | 3. Added water‚ H2O (l). | stayed the same | 4. Added sodium hydroxide‚ NaOH (aq). | changed consistency‚ gel-like | 5. Heated the
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[Teacher] [Course] [Date] Catalase Lab Report An enzyme is something that helps to speed up a chemical reaction. The enzyme changes from reaction to reaction‚ but it always has the same impact. However‚ certain variables may cause the enzyme to have a more or less significant impact on the speed of each reaction. One of these variables that changes the effectiveness of an enzyme is temperature. There is an optimal functioning temperature for each enzyme in each reaction‚ depending on the desired
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