PURIFICATION OF LACTATE DEHYDROGENASE FROM CHICKEN MUSCLE TISSUE Abstract The enzyme lactate dehydrogenase (LDH) catalyzes the last step of anaerobic glycolysis that is important for the normal function of the body. Purification of LDH is essential to understand its structure and function. The purpose of this experiment was to extract and purify LDH enzyme from chicken muscle tissue using a variety of various. Analytical methods such as activity and protein assay were employed to determine
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˚C for future use. Kinetics Measurements Absorbance values were measured at 400 using a Thermo Scientific Genesys 20 spectrophotometer. Measurements were taken at 23 ˚C. The kinetic samples were created in 2 mL microcentrifuge tubes using a two-fold serial dilution. The catechol concentration of the samples ranged from 8.00 mM to 0.065 mM. Eight uninhibited samples were prepared by mixing 10 μL of extracted enzyme
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Determination of Florfenicol in Catfish by UV-mediated High Performance Liquid Chromatography (HPLC) 1. Introduction 1.1 Description of the Analyte Florfenicol (C12H14Cl2FNO4S) is a derivative of thiamphenicol. Its structure is identical to that of chloramphenicol – a synthetically developed antibiotic for veterinary use‚ such as treating a wide range of bacterial infections (Hayes p. 7). It is commercially available as 50 percent of Aquaflor® premix‚ which has been approved for the control of bacteria
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PUSAT PENGAJIAN TEKNOLOGI INDUSTRI IMG 103/3 FOOD CHEMISTRY LAB REPORT Experiment 3 : Qualitative Test for Carbohydrate Date of Experiment: 27/03/2013 Date of Submission: 17/04/2013 Submitted by: Name: Te Hui Min Matric No.: 115615 Group: 4 Title Qualitative test for carbohydrate Introduction Carbohydrates are essential in foods as an energy source (starch is the main source of human calories)‚ a flavouring (simple sugars are usually sweet) and as a functional
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Biology 160 Lab Manual Tacoma Community College Last update 06/03/2013 This page left intentionally blank Table of Contents Table of Contents i Laboratory 1: Principles of the Scientific Method 4 In-Lab Report Sheet 12 This page has been left intentionally blank 27 Laboratory 2: Scientific Methodology & Enzyme Activity 28 In-Lab Report Sheet 28 Objectives 33 Introduction 33 Parts of the Swift M5 Microscope 33
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metal ions as well as the concentrations of Cu 2+ and Fe 3+ ions (Na+‚ K+‚ Ca++‚ Ba++‚ Sr++‚ Cu++‚ and Fe+++). We will be using the techniques
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Boys’ Code of Conduct In the article “How Boys Become Men”‚ written by John Katz‚ he analyzes situations he experienced while growing up. Katz believes that boys become men by not showing fear or feelings and not ratting on others. As in the novel‚ The Other Wes Moore‚ both Weses are forced to become men at an early age. They experience events that push them to make decisions that make them grow and think like men do. Katz details the maturation process for boys‚ as the book‚ The Other Wes Moore
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Transformation of E. coli by plasmid DNA 1. Table showing the results from the selective plates |Plate |Plasmid |Contents of plates |Number of colony | | | | |White |Blue | |1 |Ligation mixture |Ampiclillin + X- gal + IPTG |10 |0
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Ravindra Nandigam‚ Ph. D.‚ South Texas College Name: Course Number: Semester: Phys 1417 Physical Science II‚ Homework #5 Instructions: 1. Type your name‚ Course number (=PHYS 1417.V01)‚ and Semester (=Summer I‚ 2013). 2. Type your answers. Include questions. 3. Make sure that all parts of each question are answered. 4. Turn in. Chapter 25 Rocks and Minerals 1. List the 8 elements which make up about 98% of Earth’s entire mass. 2. The most abundant element in the Earth’s interior is __________________
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Yeast Lab Report Guidelines 1. Lab reports are to be computer-generated and double-spaced. All sections of the report must be written in paragraph form. 2. Do not use encyclopedias (Internet or otherwise)‚ dictionaries ((Internet or otherwise)‚ or personal web pages as sources for the report‚ this includes Wikipedia. You may use a textbook‚ lab manual‚ and/or article(s) in a published journal. You can find journal articles by going to the library website: http://www.lib.clemson.edu/ and selecting
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