the average amount of absorbance for each tube‚ containing different levels of pH. Tube 2 had an acidic pH level‚ Tube 3 had a neutral pH level‚ and Tube 4 had a basic pH level. It is indicated that the absorbance rates were the highest for the neutral pH level‚ with a final absorbance rate of 0.166. This was followed by a basic pH (0.106). The acidic pH level had the least amount of absorbance with a final absorbance rate of 0.069. This reinforces the idea that the ALP enzyme worked best under conditions
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BRADFORD ASSAY Calculation Formulas: Absorbance of BSA = A595 nm Raw Data = Average of A595 nm of three wells ÷ 3 Example: A1+A2+A3 ÷ 3 In this case‚ 0.365+0.374+0.453 ÷ 3 = .397 Corrected Data =( (A595 nm well) – BackGround) or (Raw Data – Background) Background = negative control = Bradford Reagent + No Protein Background of this standard curve = A595 nm of well ‘A’ = .397 We used well ‘A’ as our negative control. DATA RAW DATA
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May 1‚ 2013 Enzymes as Drug Targets Enzymes are defined as any of numerous proteins produced in living cells that accelerate or catalyze the metabolic processes of an organism. Enzymes are usually very selective in the molecules that they act upon‚ called substrates‚ often reacting with only a single substrate. The substrate binds to the enzyme at a location called the active site just before the reaction catalyzed by the enzyme takes place. Enzymes can speed up chemical reactions by up to
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Hugh Kim Lab Report: Stoichiometry Lab 1. Prelab Part1. 1) Create no waste = The principle that encourages chemists to not create waste at the first place rather than cleaning it up afterwards effectively shifts the chemistry more environmentally conscious‚ as creating no waste would make the experiment efficient; the reactants will be reduced to only the essential ones and the product will be maximized‚ a change that would make the experiment economic. Also‚ if chemists aim to
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60 seconds. The colour change was compared with the colour chart on the Uristix® bottle. The detection of glucose in urine is based on the presence of two enzymes‚ glucose oxidase and peroxidase. The first enzyme‚ glucose oxidase‚ catalyses the formation of gluconic acid and hydrogen peroxide from the oxidation of glucose. The second enzyme‚ peroxidase‚ catalyses the
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Physics Lab Report Format General Remarks: Writing a lab report is the only way your TA will know what you have done during the lab and how well you have understood the process and the results. Part of your lab experience should be learning how to organize and present your work in a scientific way. There is no framework that can be used as a “one size fits all”‚ therefore this sample lab report should only be used as an example. Any lab report should have the following features:
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Engineering Laboratory (Lab) Report Department of Engineering Faculty of Engineering and Science‚ University Tunku Abdul Rahman (UTAR) 1. Introduction Laboratory (Lab) reports are the most frequent document written by engineering student. These reports can contribute a significant amount of marks and yet little time or attention is devoted in understanding on how to write them well. The aim of this document is to provide a general guideline on writing a lab report. A lab report should not be
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later! Protein Portion 1. OK‚ so remember that back in the day‚ we had protein and nucleic acid resulting from a centrifugation…Well‚ now we’re dealing with the protein portion‚ which is solid 1. We take half the protein and add pancreatic enzyme a. This enyzme will hydrolyze the protein into its amino acid subunits a. This simulates how the hydrolytic process is carried out naturally‚ because in real life it is done with enyzmes! 1. And the other half of the protein
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1. Introduction: The goal of this lab was to demonstrate the microbiology technique of serial dilutions and how they can effectively be used in experiments. E. coli cells were exposed to UV light for various amounts of time in order to asses the effect on growth and thereby mutations since this cell was modified to not have photolyse no uvr genes for DNA repair and thus can only use the SOS response. Each group then diluted the cells accordingly based on their UV exposure time and counted the cells
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Enzyme Lab Daniyal Abdali (Rachel Lee) (Sarina Dolch) SBI 3UI Mr. Vrabec October 20‚ 2009 Test #1 * Add a small piece of cracker in test tube #1 and add Lugol’s solution. Observation #1 * The cracker turned a black colour when the Lugol’s solution was added to it. This was a positive result‚ meaning that the cracker contains starch. Test #2 * Add a bigger piece of cracker in test tube #2‚ add 5 mL of Benedict’s solution‚ place in a boiling water bath‚ and record observations
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