It was hypothesized that if the animal was larger‚ then it would have larger hemoglobin. It was thought that larger mammals would need more oxygen-rich hemoglobin to support healthy function. This lab did not support this hypothesis. The results in table 1 displayed that the hemoglobin of all mammalian samples traveled about the same distance. This trend can be seen in graph 1 as the mammalian hemoglobin samples migrated the same distance. Because the proteins traveled around the same distance it
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media The filtrates obtained from above experiment were used for extraction and estimation of citrinin. Briefly‚ the citrinin was extracted thrice with chloroform (1:1 v/v). All the three extractions were pooled and concentrated in vacuum at 40°C using a rotary evaporator. The crude extract was then diluted in minimum amount of chloroform (2ml) and citrinin was estimated by thin layer chromatography (TLC) according to Razak et al.[16] Subsets of citrinin obtained were estimated by High performance
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In this lab we employed various assays utilizing a biuret reagent‚ coomassie brilliant blue reagent‚ and ultraviolet light in order to determine the identity of six unknown solutions and the concentration of a bovine serum albumin sample. We were given three samples that lacked protein‚ and three samples containing proteins‚ and using a spectrophotometer we assessed the amount of light absorbed versus the light transmitted‚ based on the principles of the Beer-Lambert Law. The three proteins used
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Abstract: Bacteria consists of a large domain of prokaryotic microorganism in which can fight of antibiotics allowing the bacteria to become antibiotic resistant. In this lab report‚ we discover the most effective disinfectant that would work best in killing the harmful bacterial strain‚ Bacillus subtilis. KB testing or disc diffusion antibiotic sensitivity testing is measured through the diameter in millimeters to find how resistant the antibiotic to the bacteria. The hypothesis of Windex fell correctly
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synthesis‚ and in fireflies‚ also known as lightening bugs‚ bioluminescence (Biology Book). Bioluminescence is the process in which living organisms convert chemical energy into light (Branchini‚ 2008). In the fireflies case the reaction involves the enzyme luciferase which lights up a lantern-like mechanism under the tip the wings and attached to the end of the body of the firefly. It is presumed that the firefly uses the lantern to attract a mate. The goal in this experiment was to test the effect
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during the lab session Procedure Part A This lab was performed by the group members as they closed both low and high flow rotameters (Fig-2) and filled the tank with water. Then‚ the large rotameter was opened when the water in the tank had reached the internal baffle‚ so the flow from the tank was reduced by doing so. One member was in charge of adjusting the level of the water so it was always maintained over the baffle’s level during the lab (Fig-1). The first part of the lab was done
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CO2 Lab Report Intro Alka-Seltzer tablets are used to treat cold/flu symptoms as it produces CO2 when dissolved in water. The goal of this lab is to calculate the amount of NaHCO3 is needed to produce enough CO2 gas to fill the room. Experimental One Alka-Seltzer tablet was weighed on the analytical balance. Then a piece was measured out to be between 0.21 and 0.28 grams. 500mL of distilled water was added to a 600mL beaker. 80mL of water was mixed with 20mL of 6M HCl in a 150mL beaker. A
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Lab Report: To investigate the effect of temperature of the permeability of cell membranes DCP (Data Collection and Processing Section) Raw Data Absorbance of the colored beetroot solution at 565 nm of different temperatures of 30℃‚ 40℃‚ 50℃‚ 60℃‚ and 70℃ Temperature (℃) (± 0.1℃) Absorbance of the colored beetroot solution at 565 nm (± 0.001 AU) Trial 1 Trial 2 Trial 3 Trial 4 Trial 5 30 0.160 0.129 0.136 0.135 0.128 40 0.481 0.343 0.376 0.491 0.410 50 0.386 0.597 0.378 0.743 0
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A company has specified that the acceptable size of a knob that is to be produced is (0.140 ± 0.003) inches. In this lab‚ the dimensions of the knobs produced in one day are tested for discrepancy in order to pass control testing. This lab also reports the probability that knobs with the acceptable dimension will be produced. MATERIALS AND METHODS Using Microsoft Excel‚ a histogram was created displaying the three documented dimensions of 15 batches of knobs. This histogram was obtained in order
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structure of the enzyme is mainly dependent on the active site and variable groups. Extreme temperatures or extreme pHs can alter the structure of an enzyme. Enzymes function to lower the activation energy to break the bonds. They achieve this by putting stress and pressure on the bonds or creating a microenvironment for the substrate. Enzymes are regulated by inhibitors or activators and can be inhibited by the products of the reaction‚ called feedback inhibition. Enzymes are catalytic proteins;
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