Effect of pH on Enzyme Activity A piece of Solanum tuberosum (potato) was removed and mixed with distilled water in a blender. The resulting solution was filtered through multiple layers of cheese cloth to filter out the liquid by eliminating any large pieces in the solution. The solution created was catechol. Five different solutions were prepared as blanks with each test tube containing 6.0mL of a different pH (pH 4‚ pH6‚ pH7‚ pH8‚ pH10) of phosphate buffer‚ 1.0mL of the enzyme and 1.0mL of water
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The objectives of this experiment were to investigate the activity of enzymes‚ components that influence the enzyme’s activity‚ identify an unknown phosphatase‚ influence of inhibitors‚ and determine if inhibition is competitive or noncompetitive. A spectrophotometer evaluated the measurement of color change over a period time due to product being formed. Determining unknown phosphatase and effects from different inhibitors were determined by varying the pH and substrate concentrations. The unknown
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Ayaan Nadeem SBI 4UO-A Tuesday‚ February 27‚ 2013 Ms. Balmer Pineapple Jelly Enzyme Lab Discussion After completing the Pineapple Jell-O Enzyme lab‚ the final results were that the canned pineapple formed the jelly while the fresh pineapple did not. Pineapple In order for this to have occurred‚ there has to be a comparison between fresh and canned pineapple in terms of their physical and chemical properties. The physical properties of fresh pineapple are that it is sweet‚ ripe and raw.
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ABDOMEN WRITE-UP Examiner : Kelley Goff‚ RN Patient Initials : KJ Age: 50 Gender: Female Reason for visit: recent severe heartburn I. Health History 50 year old female in good health. No history of chronic illness. Smoker 1ppd x 24 years. Total hysterectomy at age 45. No other surgeries. Currently on no prescribed medications. No history of heart problems‚ lung problems‚ no urinary or bowel issues. No known kidney disease or liver disease. Family history of stroke (mother)
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Jordan Alyce Norris November 7‚ 2014 Biology 111-J3 Predicting Patterns of Inheritance Using Monohybrid and Dihybrid Crosses Introduction This experiment was on Mendelian Inheritance; which is the inheritance of biological features that are based on the rules proposed my Mendel. The purpose of this experiment was to show how to predict patterns of inheritance by using Punnett Squares to perform monohybrid and dihybrid crosses. Two crosses were performed. Cross one consisted of heterozygous alleles
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Abstract: Enzymes‚ molecules that speed up chemical reactions‚ are specific to one substrate. In this experiment the substrate hydrogen peroxide and the enzyme catalase will be used. The higher the concentration of potato extract‚ or catalase‚ the faster the reaction and the more substrate present will result in a decrease in the time of the reaction. The amount of concentrations of enzymes and substrates are changed to determine if the reaction is further catalyzed by a greater concentration of
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concentration of substrate‚ on the activity of the enzymes. By conducting these three separate experiments also‚ three graphs are able to be obtained where the trend of each factor affecting on the enzyme activity is shown and described clearly. II. Hypothesis Experiment 1 (Effect of Temperature): As the temperature increases‚ the height of the bubble will increase too‚ indicating a faster rate of reaction. Experiment 2 (Effect of pH): Enzymes are affected by changes in pH. Extremely high or
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BIO 5 Lab Report: Lactase Enzymes Enzymes are biological catalysts or assistants. Enzymes consist of various types of proteins that work to drive the chemical reaction required for a specific action or nutrient. Enzymes can either launch a reaction or speed it up. The chemicals that are transformed with the help of enzymes are called substrates. In the absence of enzymes‚ these chemicals are called reactants. Enzymes are thought to have an area with a very particular shape. When a molecule of
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Factors Affecting Enzyme Activity Abstract: In the following lab factors affecting enzyme activity‚ temperature‚ pH‚ enzyme concentration‚ substrate concentration and surface area will be tested on a beef liver enzyme to see if there will be any effect of performance. By doing 2 or more trials the results will show whether there is an effect to the enzyme from the following factors or not. Some of the factors may denature the enzyme and some will do nothing. Using a table qualitative and the
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Starbucks Charles Hunt Write-Up 2 November 22‚ 2013 At Starbucks we have a different view of the coffee experience. We developed our own value proposition based on our ideals and our belief that coffee drinking did not need to be brown water in a paper cup. At Starbucks we have three core components to our value proposition‚ great coffee‚ a unique and inviting atmosphere designed for people to linger in our stores and to provide great service through customer intimacy. Understanding
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