Practical: The effect of pH on enzyme activity Rationale Amylase is an enzyme involved in the digestion system which catalyses the breakdown of starch into sugars. It is not only present in human saliva but also in the pancreas‚ where it hydrolyses dietary starch into disaccharides and trisaccharides which are converted by other enzymes to glucose to supply the body with energy. pH has an effect on the activity of all enzymes‚ including amylase. As the pH level increases‚ the enzyme activity increases
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CHAPTER 4: ENZYMES Enzymes are biological catalysts. There are about 40‚000 different enzymes in human cells‚ each controlling a different chemical reaction. They increase the rate of reactions by a factor of between 106 to 1012 times‚ allowing the chemical reactions that make life possible to take place at normal temperatures. They were discovered in fermenting yeast in 1900 by Buchner‚ and the name enzyme means "in yeast". As well as catalysing all the metabolic reactions of cells (such as respiration
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Ye Tao BISC220-13155 The Effect of Temperature on the Digestion of Starch by Activity of Enzyme α-Amylase: Observation of Rate of Starch Disappearance through Iodine Test Introduction An enzyme is a type of protein that‚ through its own structure including hydrogen bonds‚ acts like a biological catalyst and is able to accelerate the biochemical reaction rate by lowering the activation energy of the whole process‚ without which cells could hardly practice any physiological functions within human
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Investigating the effect of pH on amylase activity Aim The aim of the experiment is to determine the effects of different pH and the rate of reaction on fungal amylase and starch. Introduction The enzyme amylase is found in the human body‚ it catalyses the hydrolosis of internal glycosidic bonds in polysaccharides‚ the breakdown of starch into sugars. Amylase is present in human saliva‚ where it initiates the chemical process of digestion. Enzymes work best at an optimum pH of 7 which is the bodies
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Joshua McMahon IB Chemistry Matt Chase 3A 2/13/15 Finding the PH of different concentrations of orange juice. Research Question By diluting concentrated orange juice with water‚ can you significantly change the PH value? Introduction Across the world‚ it is thought and widely accepted to believe that Orange juice is the cure to many terrible diseases. However‚ this may not be true anymore. It has been said that Oranges are very high in vitamin C‚ therefore one should drink it every morning
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2014 Biology 1 Cellular Processes Lab Section 903 Tianna Clarke Materials and Methods Part I – Restriction Enzyme Digestion To begin this experiment‚ the DNA molecules must be cut into smaller fragments with distinct enzymes called Restriction Enzymes through a process called Restriction Enzyme Digestion. Four microtest tubes were labeled 1 through 4 and added 10 µl of Enzyme Reaction Buffer to each of the four reaction tubes using a micropipette. DNA‚ and Enzyme 1 and 2‚ were then added to the
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This lab is adapted from the canned denatured pineapple experiment. Instead of investigating the effect of canning on enzyme activity‚ we look for the presence of the substrate. This is a useful teaching lab for all the criteria and requires very few resources. Background Information: Gelatin is made from a protein called collagen which comes from the joints of animals. Gelatin may be dissolved in hot water. As the dissolved gelatin mixture cools‚ the collagen forms into a matrix that
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Part I - Introduction Enzymes are proteins that act as catalysts to regulate metabolism by selectively speeding up chemical reactions in the cell without being consumed during the process. During the catalytic action‚ the enzyme binds to the substrate – the reactant enzyme acts on – and forms an enzyme-substrate complex to convert the substrate into the product. Each type of enzyme combines with its specific substrate‚ which is recognized by the shape. In the enzymatic reaction‚ the initial rate
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Introduction • Lactose intolerance is actually a very common condition. People will lactose will experience digestive problems when they eat the main sugar found in milk and dairy products. People can acquire lactose intolerance at any point in their life. This can have a negative effect on the quality of life. Causes • Lactose intolerance takes place when your small intestine doesn’t produce enough of an enzyme called lactase to digest milk sugar called lactose (Lactose intolerance Causes‚ 2016)
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Jennifer Anatomy and Physiology Enzyme Lab Report Introduction The chemical reaction that is being studied is the hydrolysis of starch. The enzyme that is being studied is amylase. This experiment is looking at the effect of temperature on the rate of the enzymatic hydrolysis of starch. My hypothesis is that the higher the temperature the faster the hydrolysis of the starch would occur. The rationale behind this is that heat
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