Biology Enzymes IA Design Introduction: Enzymes are globular proteins‚ they are responsible for most of the chemical activities of a living organism. They act as catalysts‚ substances that affects the reaction of other substances without being destroyed or altered during the process. They are extremely efficient in the body system of living organisms‚ one enzyme may catalyse over a thousand chemical reactions every second. But there are certain conditions that need to be fulfilled in order for the
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Experiment 1 : Design and preparation of buffers effective at different pHs Abstract The body uses natural buffers to maintain the many different pH environments in our body. This is important for optimum activity of our enzymes. When doing experiments in vitro using these enzymes it is important to mimic intracellular conditions using artificial buffer systems in order to obtain accurate results. In this experiment the buffering properties of three artificial buffer systems containing acetic
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Lab 1: Effect of pH on sodium benzoate‚ a food preservative Sheikh M Zakaria Person no. 36295651 TA: Synthia Gratia Date of Submission: 05/29/13 Abstract Sodium Benzoate is a common food preservative used in food products such as jams and jellies‚ soft drinks‚ pickles‚ condiments etc and in tinned products in the market. This experiment aimed to determine whether benzoic acid is formed from it’s superior soluble form sodium benzoate in stomach acid‚ which is simulated by HCl (pH=2). It has
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Running head: LAB 5: INVESTIGATING AN ENZYME-CATALYZED REACTION Lab 5: Investigating an Enzyme-Catalyzed Reaction September 24‚ 2014 Principles of Biology 120.601 Mrs. Annemarie Duncan Abstract: (Burmania) This experiment was performed in order to examine ways in which a potato catalase enzyme reacts to various assays with differing variables. To do so a baseline assay (undiluted extract and room temperature H2O2) was used within the experiment with only one other variable changed in
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Glucose During Changes in Temperature and pH‚ and Manipulation of Specificity and Cofactors BIOL-1406-SL8 November 16‚ 2012 Abstract Enzymes are biological catalysts. They work by lowering the activation energy needed to initiate a chemical reaction. Enzymes work within an optimal temperature and optimal pH. Enzymes are highly specific for a single substrate. The Enzyme is usually much larger in size than the substrate it binds to. In some cases‚ an enzyme requires something called a cofactor to
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bung. 7. Take measurement of gas after 2 minutes. 8. Repeat. Investigating the effect of enzyme concentration on the rate of an enzyme-catalysed reaction. James Moore Conclusion: For the conclusion we see that in fact the hypothesis is correct. When independent variable increases so does the dependent variable. As the amount of enzyme concentration increases so will the rate of the enzyme catalysed reaction. In the human body substrate is always available therefore there is only one
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Introduction: Enzymes are a substance produced by a living organism that acts as a catalyst to activate a specific reaction. The purpose of this experiment was to figure out if the temperature of the reaction would rise‚ will the absorption rise as well. Reactions use energy‚ If there is energy than heat occurs. The Hypothesis that was figured out was‚ If the temperature rises‚ then the absorption will also go up. The Independent variable that was tested was temperature. The dependent variable that
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RUNNING HEAD: Understanding Enzymes Understanding Enzymes Amanda Chung Blanche Ely High School Abstract The researcher conducted this experiment in order to investigate how various factors effected enzymes. Those factors were the concentration of the enzyme‚ the temperature it had to work in‚ and the pH level of the solutions as well. All three were hypothesized to speed up the rate at which the enzyme acted. A series of several tests were carried out to find the answer to each problem
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I. Introduction The purpose of this experiment is to determine the pH values of acids‚ bases‚ and buffers of distilled water and 10.0 buffer using measured concentrations of Sodium hydroxide (NaOH) and/or Hydrochloric acid (HCl). Acid is a compound typically having a bitter taste and capable of nullifying alkalis and releases hydrogen ion when added to a solution‚ or containing an atom that can accept a pair of electrons from a base (McKinley‚ Dean O’Loughlin‚ & Stouter Bidle‚ 2016). Bases are water-soluble
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Core Practical: Enzyme concentrations and enzyme activity. Introduction In this experiment I shall investigate how the enzyme concentration can affect the initial rate of reaction. I will measure the effect of the enzyme in 5 different concentrations against the controlled variable of the reactant. The enzyme which will be used is different concentrations of potato and the reactant used will be Hydrogen Peroxide. Hydrogen Peroxide which will be the buffer solution is a PH of 7.2. My hypothesis
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