An experiment to investigate the effect of temperature on the rate of reaction of the Enzyme Trypsin. Aim: This investigation was on the effect temperature has on the rate that the enzyme trypsin hydrolyses its substrate‚ a protein found in milk (casein). This investigation was conducted under controlled conditions‚ the temperature being the changeable variable. Trypsin and its substrate (powdered milk which is a source of the protein casein) were heated in a water bath. The contents of the two
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An Investigation on the rate of reaction of the enzyme Catalase on the substrate Hydrogen peroxide. Plan Aim: To investigate the rate of the effect of Catalase on hydrogen peroxide. Introduction This investigation will be carried out to investigate the rate of reaction of the enzyme catalase on the substrate hydrogen peroxide. Enzymes are biological catalysts‚ which speed up the rate of reaction without being used up during the reaction‚ which take place in living organisms. They do this by
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Enzyme Specificity Using Beta-galactosidase and Alpha-galactosidase Introduction: In order to survive‚ organisms need reactions to occur at a certain time and place. Enzymes make these reactions happen. Enzymes are highly specific causing only one enzyme to catalyze one reaction. “Induced fit” explains why enzymes act this certain way. An enzyme will surround a certain substrate and form to it for the reaction to occur in the active site. If it doesn’t form to it‚ the reaction will not occur
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VARYING EFFECTS OF ENZYME CONCENTRATION ON REACTION RATES OF MALATE DEHYDROGENASE CELL BIOLOGY 13 NOVEMBER 2007 Enzymes are biological catalysts. They are proteins that speed up reactions with low concentrations. These enzyme proteins are made up of linkages of amino acids. The links coil‚ and coil again forming a tertiary structure. This structure has a groove in it called an active site. The active site is
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lab tests how temperature and pH affect how enzymes will function. The lab showed that temperature will denature an enzyme when past its optimal working temperature and won’t denature in cold temperatures‚ but have slowed molecular activity. pH will also have an affect on an enzymes efficiency‚ when out of optimal pH the enzyme will not function as it is supposed to and if to far out of the optimal pH the enzyme will change shape and no longer work. Enzymes also showed to be reusable after the experiment
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Enzymes are globular proteins that are synthesized by the ribosomes in a cell. They act as catalysts during biological reactions; therefore‚ enzymes are able to speed up these reactions without undergoing a permanent change themselves. These proteins are able to do this by lowering the activation energy of a reaction. To add on‚ enzymes require specific conditions under which they can work best. Reactions occur at faster rates when the temperature is higher. However‚ the rate of an enzyme-catalyzed
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I. Title. Restriction Enzyme Mapping of pBR322 Using Agarose Gel Electrophoresis. II. Authors. Author: Partner: Section: Thursday‚ 1:10 pm Date of Experiment: October 25‚ 2012 III. Introduction. Restriction enzymes (or restriction endonucleases)‚ originally isolated from Haemophilus influenzae in 1970‚ are enzymes within a cell that cleave foreign DNA within a specific and predictable nucleotide sequence (known as a restriction site) regardless of the source of such DNA. Such restriction
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Effects of Temperature‚ pH‚ Enzyme Concentration‚ and Substrate Concentration on Enzymatic Activity INTRODUCTION Enzymes‚ proteins that act as catalysts‚ are the most important type of protein[1]. Catalysts speed up chemical reactions and can go without being used up or changed [3] Without enzymes‚ the biochemical reactions that take place will react too slowly to keep up with the metabolic needs and the life functions of organisms. Catecholase is a reaction between oxygen and catechol
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on enzyme activity show that as the independent variable‚ pH‚ increases the dependent variable‚ percentage transmission‚ decreases. This is shown in the results as at the lowest pH‚ pH 4.0 the average percentage transmission is at its highest at 97%. At the highest pH‚ pH 8.0 the average transmission is 78.5%. This is also supported from the graph as it produces a negative gradient showing that as the percentage transmission will decrease with an increasing pH. This happens because the enzyme trypsin
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protease enzyme on exposed developed film Plan Aim: The aim of the experiment is to find out what effect temperature has on the action of a protease enzyme on exposed developed film. Enzymes are biological catalysts. They are made in livings things built up by amino acids to make protein. Enzymes are able to speed up reactions and can repeat reactions. There are various factors that affect the activity of enzymes they are: "Y Temperature "Y pH "Y Specificity "Y Concentration of enzyme or substrate
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