Investigating the Effect of Enzyme Concentration on Pectinase in the Production of Fruit Juice In this practical‚ I shall demonstrate the relationship between the concentration of pectinase and the amount of fruit juice produced accordingly in proportion. Apparatus: Apples Pectinase solution 10 x filter paper Knife or kitchen mincer Glass rod Water bath X 1 250 ml beakers X 2 Wash bottle of distilled water 100 cm^3 measuring cylinders In the case
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Question Give an account of how enzymes work with particular reference to factors that affect their activities. Answer All living things require catalysts to catalyse the reaction which they carry out to survive. Enzymes are the catalysts of biological systems without enzymes reactions in cells would occur all much to slow a rate and the organism would not survive. Most enzymes‚ with the exception of some RNA catalysts‚ are proteins. They often require a co factor such as zinc or iron‚ or a complete organic
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of Reaction of Enzyme Amylase Research Question: How will changing the percentage of sodium chloride concentration affect the rate of reaction of enzyme amylase‚ measured using the absorbance of starch and iodine with a spectrophotometer. Introduction: Amylase is an enzyme that is involved in the human digestive process. Found in both the human pancreas and the human saliva‚ amylase breaks down starch into sugar so that large molecules can be easily digested1. Like all enzymes‚ amylase must
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Hydrolysis‚ Solubilization and Enzyme Inactivation D. Krlrç Apar and B. Özbek* Yrldrz Technical University‚ Department of Chemical Engineering‚ Davutpaºa Campus‚ 34210‚ Esenler/Istanbul‚ Turkey Original scientific paper Received: April 11‚ 2007 Accepted: May 31‚ 2007 The aim of this study was to investigate the influences of substrate concentration‚ enzyme concentration‚ temperature and pH on hydrolysis and solubilization of corn gluten as well as enzyme stability. The corn gluten was
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Abstract Enzymes are proteins that lower the activation energy needed for chemical reactions. The two main environmental factors that can affect the enzyme’s activity are temperature and pH‚ and each enzyme works best at a particular temperature and pH. The purpose of this enzyme kinetic experiment was to observe the effect of temperature and pH on the reaction of barley alpha-amylase enzyme with starch substrate and establish the optimum temperature and pH for this reaction. The optimum temperature
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INTRODUCTION Enzymes are biological catalysts that speed up chemical reactions‚ without being used up or changed. Catalase is a globular protein molecule that is found in all living cells. A globular protein is a protein with its molecules curled up into a ’ball’ shape. All enzymes have an active site. This is where another molecule(s) can bind with the enzyme. This molecule is known as the substrate. When the substrate binds with the enzyme‚ a product is produced. Enzymes are specific to their
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Chapters 6‚7 &31 Question 1 1 out of 1 points Some enzymes contain molecules in the active site that help facilitate chemical transformations. These molecules are called: Selected Answer: coenzymes. Correct Answer: coenzymes. Question 2 1 out of 1 points People with high cholesterol levels often take drugs in an attempt to lower their cholesterol levels. One such drug is Lipitor. How does this drug work? Selected Answer: It acts as a competitive
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Starch can be hydrolyzed into simpler carbohydrates by acids‚ various enzymes‚ or a combination of the two. The resulting fragments are known as dextrins. The extent of conversion is typically quantified by dextrose equivalent (DE)‚ which is roughly the fraction of the glycosidic bonds in starch that have been broken. These starch sugars are by far the most common starch based food ingredient and are used as sweetener in many drinks and foods. They include: Maltodextrin‚ a lightly hydrolyzed (DE
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__________________________________ AP Biology LAB 2. ENZYME CATALYSIS ESSAY 2000 The effects of pH and temperature were studied for an enzyme-catalyzed reaction. The following results were obtained. a. How do (1) temperature and (2) pH affect the activity of this enzyme? In your answer‚ include a discussion of the relationship between the structure and the function of this enzyme‚ as well as a discussion of how structure and function of enzymes are affected by temperature and pH. b. Describe
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Procedures for Part A: For Activity A‚ we first tested enzyme activity. First‚ we used an H2O2 syringe to transfer 10 mL of H2O2 into an unlabeled 60-mL cup. Then‚ we used a transfer pipet to add one mL of catalase solution into the unlabeled 60-mL cup that we put H2O2 in. After that‚ we observed the solution for one minute. Then we tested the effect of boiling on enzyme activity. First we used a transfer pipet to transfer 4 mL of catalase into a test tube. After that‚ we placed the test tube filled
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