in which jelly cubes were immersed over a 24 hour period Interpretation Written Communication of the Data C1 Pepsin is an enzyme that works in the stomach and has an optimal pH between pH 1 and 4 or in acidic conditions. From our graph it can be seen that that the lowest mean percentage light transmission for pepsin is when the buffer has a pH of 2. Trypsin is an enzyme that works in the small intestine and has an optimum pH between pH 7 and 8 or in neutral conditions. From our graph it can be
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Common Introduction: -Beer-Lambert Law found by Beer August and Johann Heinrich Lambert is the linear relationship between the attenuation of light and concentration of the material through which the light is travelling. It states the absorbance is proportional to the concentration when a parallel beam of monochromatic radiation of equal pathlength is passing through a homogenous concentration. A=εbc‚ where A is absorbance‚ ε is molar absorptivity (L molˉ1cmˉ1)‚ b is the pathlength (1cm) and c
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Amylase is an important enzyme in the human body as it allows for the consumption of starch by breaking the polysaccharide down into maltose units. All enzymes‚ including amylase‚ function best at a certain optimal pH. Therefore‚ in this experiment‚ the effect of different pHs on the reaction rate of amylase is studied. It was hypothesized that the amylase-starch reaction would proceed fastest at a pH closest to that of the body‚ or 7.0‚ since that is where the enzyme normally functions. Lower
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the acyl-enzyme is then hydrolysed by nucleophilic attack of serine on the peptide carbonyl group‚ collapse of the tetrahedral intermediate and the release of the amine component. Histidine 57 then acts as a general acid catalyst and draws a proton away from the water molecule. The OH- ion then attacks the carbonyl carbon atom of the acyl group which forms another tetrahedral intermediate which breaks down to form the carboxylic acid product. The release of this acid then frees up the enzyme to continue
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Section______________________________ Virtual Lab: Enzyme Controlled Reactions Worksheet Go to the following website to complete the virtual lab: http://www.mhhe.com/biosci/genbio/virtual_labs_2K8/pages/EnzymeControlledReactions.html Due: Wednesday‚ March 28‚ 2012 during your respective lab time 1. Which of the following does NOT apply to an enzyme: a. Catalyst b. Inorganic c. Protein d. All of the above apply to an enzyme 2. When an enzyme catalyzes a reaction: a. Substrate(s) bind in the
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Title: Investigation of the Enzymatic Effects of Materials on Hydrogen Peroxide Solution Objective: To investigate the enzymatic effect of various materials in the hydrogen peroxide solution Apparatus and Equipment: Beaker‚ Boiling tubes‚ Water bath‚ Pen knife‚ Glass rod‚ Parafilm. Materials: Fresh liver‚ Potato cubes‚ Manganese dioxide‚ Hydrogen peroxide‚ Wood splints Procedures: 1. Six fresh empty boiling tubes 1‚ 2‚ 3‚ 4‚ 5‚ 6 were labeled. 2. The liver provided was cut into
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Enzymes are proteins which serve to reduce the activation energy required for biological reactions (Russell and others 2010). This allows biologically important chemical reactions to occur rapidly enough to allow cells to carry out their life processes (Russell and others 2010). Enzymes are made of one or more polypeptide strands‚ which individually or as an associated complex take on a three-dimensional shape. When properly associated‚ these shapes form the active site and other supporting structures
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Research Question: How will an increase of FeSO4 concentration affect the activity of the enzyme catalase? Hypothesis: By increasing the concentration of FeSO4‚ there will be a decrease in the activity of the enzyme catalase because the Iron (ii) in the metallic ion will act as an inhibitor during the catalysis of the hydrogen peroxide into O2 and H2O. Introduction: We rely on oxygen to provide us with energy (ATP) and go through all the phases of aerobic respiration. However‚ oxygen can be
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Conclusion 7 8.0 Appendix 8 9.0 Reference List 10 1.0 Abstract Enzymes are catalysts to ensure homeostasis in the body [2]. Homeostasis is the tendency to maintain the internal environment [2]. Hydrogen peroxide is an oxidizing agent that can be decomposed by catalase [11]‚ the liver is able to decompose hydrogen peroxide enzymatically because it contains catalase [12]. The reaction rate of an enzyme catalysed reaction under the effect of changes in temperature was tested in this
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This lab was performed in order to discover the activity of the enzyme catecholase in different pH levels as well as its absorbance in differently concentrated solutions. A spetrophotometer was used to measure the absorbance of the enzyme catecholase in different pH solutions as well as to measure the absorbance of catecholase in solutions with different concentrations of potato juice and phosphate buffers. Absorbance of the enzyme catecholase was at an optimum level when pH was close to neutral
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