“Investigate the factors affecting the rate of yeast respiration” Lab Report Introduction The aim of this experiment was to investigate the effect of different amounts of a substrate on the respiration rate of yeast and to compare this to the effect of different amounts of glucose on the rate of yeast respiration. The substrate which I chose to further investigate was fructose. Fructose is a fruit sugar which is one of the three‚ along with glucose and galactose‚ dietary monosaccharides that
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* A reactant in an enzyme-catalyzed reaction is called a substrate. The area of the enzyme that binds to the substrate is called the active site - where the action takes place. * Cofactors and coenzymes are small molecules or ions that help enzymes to act. HSC Biology Notes – Hugh Phillips Maintaining a Balance 1.Most organisms are active in a limited temperature range: Identify the role of enzymes in metabolism‚ describe their chemical composition and use a simple model to describe their
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Glucose 6-phosphate is transferred to NADP which soon becomes NADPH. NADPH is accumulated and later used for biosynthesis. *Fate of Ribulose 5-phosphate Ribulose 5-phosphate can now proceed to different enzymatic reactions. It is acted by an enzyme (Not specified) and converting it into ribose 5-phosphate. Oxidizing it and making it into ribose 5-phosphate. Ribulose 5-phosphate and xylulose 5-phosphate can later be converted into carbohydrate intermediates (fructose 6-phosphate) which are present
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Biochemical Tests- presence or absence of specific enzymes helps us to identify bacteria -used to evaluate metabolic capabilities of specific bacteria. 1. Catalase Test- Enzyme: Catalase Substrate: Hydrogen Peroxide Reagent: Hydrogen Peroxide Product: Oxygen Medium: TSA Bubbles (+)- Catalase… No Bubbles (-)- No Catalase 2. Denitrification Test- Enzyme: Nitrate Reductase
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Yeast which is also known as Saccharomyces Cerevisiae is a unicellular eukaryotic Fungi means that is made up of one cell with a nucleus(“What is Yeast”).Yeast is a very practical product that is used in mainy way like when baking Yeast helps raise the dough and also yeast is used in the process of making wine.The Reason why yeast was chosen to was to see how yeast can metabolize different sugars and how much Co2 they release when when metabolizing.To test the yeast at the lab we used 4 different
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the enzyme? Denaturing the enzyme is important because leaving it active will increase the activity of the enzyme. 1. State the optimum pH for sucrase activity and how sucrase activity changes at more acidic and alkaline pH values. 2. State the optimum temperature for sucrase activity and how sucrase activity changes at lower and higher temperatures. 3. State how sucrase activity changes with increasing sucrose concentration. Discussion 1. Explain how pH and temperature affect enzyme activity
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Within the mitochondria CHOL is converted to pregnenolone (PREG) by the side-chain cleavage enzyme known as CYP11A1. The conversion to PREG is the rate-limiting step and the following intermediate steps are taken place in the smooth endoplasmatic reticulum . PREG is either converted to 17α-hydroxypregnenolone (OH PREG) by the 17α-hydroxylase activity of CYP17A1 or converted to progesterone (PROG) by the enzyme 3β-hydroxysteroid dehydrogenase (3β-HSD). PROG is further converted to 17α-hydroxyprogesterone
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ABSTRACT Denim and its various items like pants‚ shirts‚ jackets‚ belts‚ caps‚ etc‚ are the most preferred clothing for today’s youth. This study discusses compositions and methods of reducing or preventing back staining of indigo dyes on the weft yarn and pocket of denim garments. The dye‚ as it is removed from the denim material‚ post treatment with cellulase or by a conventional washing process may cause ‘back staining’ or ‘redeposition’ on the denim material; e.g : re-coloration of blue
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Def: An enzyme is a protein formed by the body that acts as a catalyst to cause a certain desired reaction. Enzymes are very specific. Each enzyme is designed to initiate a specific response with a specific result. There are many enzymes in the human body. In cystic fibrosis‚ the term enzyme generally refers to the enzymes created by the pancreas that are intended to initate a reaction that digests food. Structure :The basic mechanism by which enzymes catalyze chemical reactions begins with
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What affect does boiling have on enzyme activity? a. Boiling inactivates‚ or denatures‚ enzymes. Activity 2 Which tubes showed that starch or cellulose was present? a. 4‚ 5 and 6 Which tubes tested positive for the presence of reducing sugars? a. 1‚ 2‚3 and 7 What was the affect of freezing tube 1? a. Freezing had no affect How does the effect of freezing differ from the affect of boiling? a. Freezing does not restrict enzyme activity Does amylase use
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