Experiment 4: Enzyme Kinetics. Results/Discussion Week 1 Part A: Table 1. Enzyme activity for each assay of 4-nitroaniline formation. Rate of 4-nitroaniline formation Name of trial Abs/sec Abs/min M/min mol/min µmol/min #1 0.00003 0.0018 2.05x10-7 2.15 x10-10 2.15 x10-4 # 2 0.00010 0.0060 6.81x10-7 7.15x10-10 7.15x10-4 # 3 0.00020 0.0120 1.36x10-6 1.43x10-9 1.43x10-3 # 4 0.00030 0.0180 2.00x10-6 2.10x10-9 2.10x10-3
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Enzyme Kinetics Marcos‚ Nelissa S. Institute of Chemistry‚ University of the Philippines‚ Diliman‚ Quezon City 1101 Philippines ABSTRACT The rationale of the experiment is basically founded in the concept of reaction rates as affected by enzyme‚ and how the enzyme works is competed by a competitive inhibitor‚ thereby impeding the forward reaction. In this experiment‚ o-diphenol oxidase‚ an enzyme that causes the browning in fruits‚ was extracted from banana and reaction rate of this was established
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Enzymes are biological catalysts‚ large protein proteins that have a very specific three-dimensional shape which makes them highly specific and only work on one or a few similar chemical reactions. Enzymes themselves are not consumed in the reaction‚ but they help attract substrates into correct position to undergo chemical reaction. Enzymes greatly speed up the rate of biological reactions by lowering the energy of activation. To get a sense of the speed and efficiency of enzymes‚ substrates can
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Action of enzymes as catalysts in biochemical processes * Enzymes acts as catalyst and increase the rate of all the chemical reactions. * Enzymes are also described by two properties like all other catalysts. It composed of two main functions. * The first function is that‚ they increase the rate of chemical reactions by without consumed themselves or undergo any change or alteration in the reaction. . ( Zemitec et‚al 2008). * The second function is‚ they increase reaction rates without
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Title: Enzyme Activity Aim: To investigate the activity of the enzyme catalase in liver and potatoes‚ and to investigate the effect of temperature‚ surface area‚ pH and certain chemicals on the activity of catalase. Equipment: Dilute hydrochloric acid solution x 1ml 10 volume hydrogen peroxide x 100ml Copper sulphate solution x 1ml Aluminum nitrate solution x 1ml Zinc nitrate solution x 1ml dilute NaOH solution x 1ml Mortar and pestle x 1 25ml Beaker x 1 Hot plate x1
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BABS1201 Group Enzymes Project Group Protocol Including Equipment and Reagents List Lab Day: Wednesday Lab Time: 10am – 1pm Are you in Lab G20 (furthest from BSB Student Office) or Lab G21 (closest to BSB Student Office)?: Demonstrator Name: Daniel Winters Names of Group Members: Johnny Nguyen‚ Therese Pham‚ Linda Tang Name of Enzyme You are Investigating: Amylase Brief Background: Amylase is a digestive enzyme‚ produced mainly by the salivary glands and the pancreas‚ to break down
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relation to its secretory and absorptive functions. (a) Explain the characteristics of enzymes. (b) Explain the lock and key theory of enzyme action. (c) Locate and explain the role of important digestive enzymes. TASK 5: Enzymes are involved in anabolic and catabolic reactions: a) Define the terms anabolic and catabolic reactions b) Give 3 examples of anabolic and 3 examples of catabolic reactions. Referencing Harvard - page numbers for all references Requested Sources
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Immobilized Enzyme Immobilized Enzyme * Enzymes which are attached to small bead made of alginate are called immobilized enzyme * However‚ whatever the nature of an immobilized enzyme‚ it must comprise two essential functions‚ namely the non-catalytic functions (NCF) that are designed to aid separation and the catalytic functions (CF) that are designed to convert the target compounds within the time and space desired * Immobilized enzymes might meet the increasing demand by manufacturers
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Enzyme Catalase Activity in Reaction with the Substrate Hydrogen Peroxide Abstract We performed these experiments to observe the effects of enzymes on the rate of reactions. We tested and compared the activity of the enzyme catalase on the substrate H2O2 in various states and percentages‚ and observed the absorption values of the enzyme-substrate relationship at different concentrations. Our results show that the more substrate available‚ the quicker the reaction will happen except in one test
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Study Guide for Unit V Energy and Enzymes. What is cellular work? Why must living things utilize energy with maximum efficiency? Define energy. Why do living things need a constant input of energy? Where does all energy in the biosphere originate? Define kinetic and potential energy. (Give biological examples). What are the two laws of thermodynamics? Give examples. What is entropy and what is the law of entropy? How do living things resist the law of entropy? Give biological examples. Define
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