Introductory Biology 1 Biology 1003 Fall Term 2011 Lab Number: 3 Title: Cell Energetics: Enzyme Role in Biological Reactions Name: Brandon Moore Student Number: 100819124 Lab day and time: Wednesday pm Date: Wednesday November 23‚ 2011 Introduction Enzymes are a key aspect in our everyday life and are a key to sustaining life. They are biological catalysts that help speed up the rate of reactions. They do this by lowering the activation energy of chemical reactions (Biology
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Lab 4 Quiz 1. Enzymes and what is their function? a. Enzyme: biological catalysts that regulate chemical reactions that occur in cells. Responsible for things such as converting food to energy‚ replace old damaged tissues‚ disposal of cellular waste products. Are responsible for lowering the activation energy. 2. Most enzymes are proteins with three-dimensional shapes determined by their amino acid sequence. 3. Substrate – is a reactant molecule that binds to the highly specific active site
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Enzymes are proteins or nucleic acids that catalyze reactions. They are able to speed up reactions by reducing the activation energy of a reaction. Each kind of enzyme has a specific shape that matches its substrate so it can bind to its active site. Enzymes convert their substrates into a product. Enzyme activity are affected by factors such as temperature‚ pH‚ and time. If an enzyme is exposed to extreme heat‚ it will become denatured‚ that is‚ to become deformed and lose its original shape which
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Investigating the effect of changing substrate concentration on the activity of the enzyme catalase The aim of this experiment is to examine how the concentration of a substrate (hydrogen peroxide) affects the rate of reaction of an enzyme catalyse (found in liver cells) Research Question: how does changing the concentration of the substrate affect the rate of reaction of the enzyme catalyse? Hypothesis: As the concentration of the substrate increases‚ so does the rate of reaction until the reaction
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Use of Enzymes in Food Industry - Food industry utilizes a variety of enzymes for processing of various foods‚ e.g.‚ production of various types of syrups from starch or sucrose (a- and β- amylases‚ glucamylase‚ pullulanase‚ invertase‚ and glucose isomerase)‚ meat/protein processing using proteases‚ removal of glucose and or molecular oxygen (O2) using glucose oxidase and catalase‚ use of lactase in dairy industry and use of enzymes in fruit juice and brewing industries. Glucose oxides are obtained
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Purpose: To find how pH and temperature levels affect the enzyme Background: Enzymes are a type of protein vital to sustaining life‚ it works with vitamins and minerals as a biological catalyst‚ which lowers the activation energy for a reaction to occur. Each individual type of enzyme does a specific job‚ and they do not die‚ they are reused. For example‚ catalase is an enzyme found in almost all living cells that will break down hydrogen peroxide and turn it into water and oxygen. The
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Title: Catalase enzyme detection Objective: To understand the function of catalase in cells that produce the enzyme‚ interpret the results of a catalase test and know their value in differentiating bacteria. Materials: 1 clean microscopic slide‚ 3% H2O2 solution‚ swabs. Micrococcus luteus‚ Enterococcus faecalis‚ patient G Procedure: 1) Scrape some cells off from each bateria to the slant and place them on glass slide. 2) Place one or two drops of H2O2. Watch for bubbling as an indication of
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EFFECT OF TEMPERATURE‚PH AND SUBSTRATE CONCENTRATION ON ENZYME ACTIVITY | Aim To investigate the effect of Temperature‚Ph and substrate concentration on the rate of enzyme activity. Hypothesis
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Enzymes are catalysts that are used to speed up chemical reactions. Adding inorganic catalysts to reactants increase the rate of most chemical reactions. However‚ all enzymes are different and there are numerous amounts. Many enzymes are essential for life and reactions would not happen rapidly to maintain life with the help of enzymes. Specific enzymes lower the activation energy for specific reactions and shapes. Activation energy is required to start a chemical reaction. This occurs when energy
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on enzyme activity Objective To study the effect of two heavy metal ions‚ lead (II) ions and silver ions on the enzyme activity of invertase. Introduction Hypothesis: The rate of enzyme reaction of invertase decreases and the reaction eventually stops as the heavy metal ions inhibit the enzyme reactions. Biological principle: Heavy metal ions are non-competitive inhibitors. They do not compete with the substrates for the active sites of enzymes. They attach to other parts of the enzyme molecules
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